Physical Sciences and Mathematics Commons^™

Partial Characterization Of Two Moderately Halophilic Bacteria From A Kansas Salt Marsh, Aileen M. Johnson, Lance R. Thurlow, Sam R. Zwenger, Eric T. Gillock

The Prairie Naturalist

Two bacterial species were isolated from a salt marsh located on privately owned land in Russell County, Kansas. Water samples from the saIt marsh were streaked for isolation on tryptic soy agar supplemented with 12 % NaCI. Visual scanning of the plates revealed two prominent colony types. The two colony types were subcultured repeatedly until axenic cultures were obtained. 80th of these organisms were shown to be moderately halophilic. The organisms were characterized partially by fatty acid methyl ester analysis, 16S rRNA sequencing, and scanning electron microscopy. These studies revealed that the bacteria previously were unreported members of genera Marinococcus …