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University of Nebraska - Lincoln
Department of Chemistry: Dissertations, Theses, and Student Research
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Full-Text Articles in Physical Sciences and Mathematics
The Application And Development Of Metabolomics Methodologies For The Profiling Of Food And Cellular Toxicity, Jade Woods
Department of Chemistry: Dissertations, Theses, and Student Research
Metabolomics is a rapidly growing field of study. Its growth reflects advancements in technology and an improved understanding of the impact of the environment on metabolism. As a result, metabolomics is now commonly employed to investigate and characterize human and plant metabolism. The first chapter of this thesis provides an introduction to metabolomics and an overview of the protocols for sample preparation, data collection and statistical analysis. The second thesis chapter describes in explicit detail the step-by-step process of extracting and analyzing metabolites collected from mammalian cells, specifically brain tissue with a focus on Parkinson’s disease. The chapter highlights important …
How Oxygen-Binding Affects Structural Evolution Of Even-Sized Gold Anion Clusters. (Size Range 20 To 34), David Brunken-Deibert
How Oxygen-Binding Affects Structural Evolution Of Even-Sized Gold Anion Clusters. (Size Range 20 To 34), David Brunken-Deibert
Department of Chemistry: Dissertations, Theses, and Student Research
We report a joint anion photoelectron spectroscopy (PES) and theoretical study to investigate the effect of O2-binding on the mid-sized even-numbered gold clusters, Aun− (n = 20−34), a special size region of bare gold clusters that entail rich forms of structural evolution and transformation. Specifically, within this size range, bare Au20− is a highly-symmetric pyramidal cluster, bare Au21-25− are flat-planar or hollow-tubular clusters, bare Au26− is the smallest core-shell gold cluster, while bare Au34− is a magic-number/fluxional core-shell cluster with the high-symmetry tetrahedral Au4 core. In light of the strong …
Analysis Of Hydroxychloroquine Interaction With Serum Proteins By High Performance Affinity Chromatography, Kyungah Suh, Sadia Sharmeen, David S. Hage
Analysis Of Hydroxychloroquine Interaction With Serum Proteins By High Performance Affinity Chromatography, Kyungah Suh, Sadia Sharmeen, David S. Hage
Department of Chemistry: Dissertations, Theses, and Student Research
Personalized medicine or precision medicine has been an emerging market for better treatments of patients under varying physiological conditions.To improve the development of personalized medicine, a higher understanding of drug-protein binding between serum transport proteins and the unbound form of pharmaceuticals is needed. Human Serum Albumin (HSA) and Alpha1-acidglycoprotein (AGP) play a crucial role in pharmacokinetics as they are two most abundant transport serum proteins in body circulatory system. Inter-individual variabilities of serum proteins, such as the physiological condition of patients, have been believed to alter the drug-serum protein binding. Fig. 1 shows an example of the cause of inter-individual …
Evaluation Of The Overall Binding Of Acetohexamide And Tolbutamide With Methyl Glyoxal-Modified Hsa By High-Performance Affinity Chromatography, Ashley G. Woolfork, David S. Hage
Evaluation Of The Overall Binding Of Acetohexamide And Tolbutamide With Methyl Glyoxal-Modified Hsa By High-Performance Affinity Chromatography, Ashley G. Woolfork, David S. Hage
Department of Chemistry: Dissertations, Theses, and Student Research
High performance affinity chromatography (HPAC) was used in a zonal competition format to investigate the overall binding of two sulfonylurea drugs with modified human serum albumin (HSA). The in vitro modifications of this protein that were examined included glycation, as occurs in the presence of elevated glucose levels during diabetes, and the formation of advanced glycation end-products (AGEs) due to the reaction of HSA with methylglyoxal (MGo). The sulfonylurea drugs acetohexamide and tolbutamide, which are used to treat type II diabetes, were each injected onto 10 mm x 2.1 mm i.d. affinity microcolumns that contained normal HSA and HSA modified …