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Full-Text Articles in Physical Sciences and Mathematics
Purification And Characterization Of Nadph-Cytochrome P450 Reductase From Lake Van Fish Liver Microsomes And Investigation Of Some Chemical And Metals' Effects On The Enzyme Activity, Müslüm Kuzu, Mehmet Çi̇ftci̇
Purification And Characterization Of Nadph-Cytochrome P450 Reductase From Lake Van Fish Liver Microsomes And Investigation Of Some Chemical And Metals' Effects On The Enzyme Activity, Müslüm Kuzu, Mehmet Çi̇ftci̇
Turkish Journal of Chemistry
NADPH-cytochrome P450 reductase was purified from Lake Van fish liver microsomes by primary and secondary DEAE-cellulose column chromatograph with 20.46 \mu M/min/mg enzyme specific activities, 54.4% purification yield, and purification of 38-fold. The purity of the enzyme was established, and its monomer molecular weight was determined by SDS-polyacrylamide gel electrophoresis. SDS-PAGE results showed a single band and the molecular weight of NADPH-cytochrome P450 reductase was 70 kDa. In addition, optimum ionic strength, optimum pH, optimum temperature, and stable pH values were determined for the enzyme in the kinetic studies performed. K_M and V_{max} were determined for NADPH and cytochrome c. …
Separation And Preconcentration Of Lead(Ii), Cobalt(Ii), And Nickel(Ii) On Edta Immobilized Activated Carbon Cloth Prior To Flame Atomic Absorption Spectrometric Determination In Environmental Samples, Zeid Abdullah Alothman, Erkan Yilmaz, Mohamed Habi̇la, Mustafa Soylak
Separation And Preconcentration Of Lead(Ii), Cobalt(Ii), And Nickel(Ii) On Edta Immobilized Activated Carbon Cloth Prior To Flame Atomic Absorption Spectrometric Determination In Environmental Samples, Zeid Abdullah Alothman, Erkan Yilmaz, Mohamed Habi̇la, Mustafa Soylak
Turkish Journal of Chemistry
The synthesis and characterization of ethylenediaminetetraacetic acid immobilized activated carbon cloth was performed in the present work. It was used for preconcentration-separation of lead(II), cobalt(II), and nickel(II) at trace levels as an adsorbent. Factors including pH, concentration and volume of eluent, sample and eluent flow rates, sample volume, and effect of coexisting ions on the solid phase extraction of analytes were examined. The preconcentration factor was 50. The detection limits for Pb(II), Co(II), and Ni(II) were 4.39, 0.99 and 0.91 $\mu $g L$^{-1}$, respectively. The adsorption capacity for Pb(II), Co(II), and Ni(II) ions was found as 11.0, 11.2, and 10.2 …