Physical Sciences and Mathematics Commons^™

Meta-Analysis Of Mesenchymal Stem Cell Gene Expression Data From Obese And Non-Obese Patients, Dakota William Shields

Masters Theses

"The prevalence of gene expression microarray datasets in public repositories gives opportunity to analyze biologically interesting datasets without running the laboratory aspect in house. Such experimentation is expensive in terms of finances, time, and expertise, which often results in low numbers of replicates. Meta-analysis techniques attempt to overcome issues due to few biological or technical replicates by combining separate experiments together to increase statistical power. Proper statistical considerations help to offset issues like simultaneous testing of thousands of genes, unintended hybridization, and other noises.

Microarrays contain light intensities from tens of thousands of hybridized probes giving a measure of gene …