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Full-Text Articles in Physical Sciences and Mathematics
Synthesis Of Novel Sugar Diamino Acids, M Thillakan, A Katsifis, D Skropeta
Synthesis Of Novel Sugar Diamino Acids, M Thillakan, A Katsifis, D Skropeta
Danielle Skropeta
Sugar amino acids (SAAs) are found in nature as good construction elements for the preparation of peptide mimetics and oiigosaccharides in drug design and development. The synthesis of SAAs is readily accomplished in few steps and more than 40 SAAs have been synthesised to date.2 Sugar amino acids with an additional amino group, the sugar diamino acid (SDAs) would represent a useful expansion to the library of SAAs available because one of the amino group and carboxylic acid is available for peptide coupling and the another amino/azide group allow to do further derivatisation via peptide or click chemistry such as …
High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting
High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting
Professor Nick E Dixon
Using aminoacyl-tRNA synthetase/suppressor tRNA pairs derived from Methanocaldococcus jannaschii, an Escherichia coli cell-free protein production system affords proteins with site-specifically incorporated unnatural amino acids (UAAs) in high yields through the use of optimized amber suppressor tRNACUA opt and optimization of reagent concentrations. The efficiency of the cell-free system allows the incorporation of trifluoromethyl-phenylalanine using a polyspecific synthetase evolved previously for p-cyanophenylalanine, and the incorporation of UAAs at two different sites of the same protein without any re-engineering of the E. coli cells used to make the cell-free extract.
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
William E. Price
The effect of organic modifiers on the separation of a number of closely related isomeric benzoic acids by capillary electrophoresis is described. It is shown that while a single modifier concentration cannot help resolve the entire electropherogram, organic modifiers do significantly enhance the resolution of parts of the separation system by comparison with 40 mmol l-1 phosphate buffer. The effects on separation and retention times are discussed in terms of the effects on electroosmotic flow and the electrophoretic mobilities of the charged solutes. The effects were found to be modifier specific, although the trends were in the same direction (ie., …
High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting
High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting
Faculty of Science - Papers (Archive)
Using aminoacyl-tRNA synthetase/suppressor tRNA pairs derived from Methanocaldococcus jannaschii, an Escherichia coli cell-free protein production system affords proteins with site-specifically incorporated unnatural amino acids (UAAs) in high yields through the use of optimized amber suppressor tRNACUA opt and optimization of reagent concentrations. The efficiency of the cell-free system allows the incorporation of trifluoromethyl-phenylalanine using a polyspecific synthetase evolved previously for p-cyanophenylalanine, and the incorporation of UAAs at two different sites of the same protein without any re-engineering of the E. coli cells used to make the cell-free extract.
Selective Reduction Of Hydroperoxyeicosatetraenoic Acids To Their Hydroxy Derivatives By Apolipoprotein D: Implications For Lipid Antioxidant Activity And Alzheimer's Disease, Surabhi Bhatia, Bianca Knoch, Jenny Wong, Woojin Scott Kim, Paul Else, Aaron J. Oakley, Brett Garner
Selective Reduction Of Hydroperoxyeicosatetraenoic Acids To Their Hydroxy Derivatives By Apolipoprotein D: Implications For Lipid Antioxidant Activity And Alzheimer's Disease, Surabhi Bhatia, Bianca Knoch, Jenny Wong, Woojin Scott Kim, Paul Else, Aaron J. Oakley, Brett Garner
Faculty of Science - Papers (Archive)
ApoD (apolipoprotein D) is up-regulated in AD (Alzheimer's disease) and upon oxidative stress. ApoD inhibits brain lipid peroxidation in vivo, but the mechanism is unknown. Specific methionine residues may inhibit lipid peroxidation by reducing radical-propagating L-OOHs (lipid hydroperoxides) to non-reactive hydroxides via a reaction that generates MetSO (methionine sulfoxide). Since apoD has three conserved methionine residues (Met49, Met93 and Met157), we generated recombinant proteins with either one or all methionine residues replaced by alanine and assessed their capacity to reduce HpETEs (hydroperoxyeicosatetraenoic acids) to their HETE (hydroxyeicosatetraenoic acid) derivatives. ApoD, apoDM49-A and apoDM157-A all catalysed the reduction of HpETEs to …
Importance Of Secondary Sources In The Atmospheric Budgets Of Formic And Acetic Acids, Fabien Paulot, Debra Wunch, John D. Crounse, G C. Toon, Dylan B. Millet, Peter F. Decarlo, C Vigouroux, Nicholas M. Deutscher, G Gonzalez Abad, Justus Notholt, Thorsten Warneke, J Hannigan, Carsten Warneke, Joost A. De Gouw, Edward Dunlea, M De Maziere, David W. Griffith, P Bernath, J L. Jimenez, Paul O. Wennberg
Importance Of Secondary Sources In The Atmospheric Budgets Of Formic And Acetic Acids, Fabien Paulot, Debra Wunch, John D. Crounse, G C. Toon, Dylan B. Millet, Peter F. Decarlo, C Vigouroux, Nicholas M. Deutscher, G Gonzalez Abad, Justus Notholt, Thorsten Warneke, J Hannigan, Carsten Warneke, Joost A. De Gouw, Edward Dunlea, M De Maziere, David W. Griffith, P Bernath, J L. Jimenez, Paul O. Wennberg
Faculty of Science - Papers (Archive)
We present a detailed budget of formic and acetic acids, two of the most abundant trace gases in the atmosphere. Our bottom-up estimate of the global source of formic and acetic acids are ~1200 and ~1400 Gmol yr−1, dominated by photochemical oxidation of biogenic volatile organic compounds, in particular isoprene. Their sinks are dominated by wet and dry deposition. We use the GEOS-Chem chemical transport model to evaluate this budget against an extensive suite of measurements from ground, ship and satellite-based Fourier transform spectrometers, as well as from several aircraft campaigns over North America. The model captures the seasonality of …
Synthesis Of Novel Sugar Diamino Acids, M Thillakan, A Katsifis, D Skropeta
Synthesis Of Novel Sugar Diamino Acids, M Thillakan, A Katsifis, D Skropeta
Faculty of Science - Papers (Archive)
Sugar amino acids (SAAs) are found in nature as good construction elements for the preparation of peptide mimetics and oiigosaccharides in drug design and development. The synthesis of SAAs is readily accomplished in few steps and more than 40 SAAs have been synthesised to date.2 Sugar amino acids with an additional amino group, the sugar diamino acid (SDAs) would represent a useful expansion to the library of SAAs available because one of the amino group and carboxylic acid is available for peptide coupling and the another amino/azide group allow to do further derivatisation via peptide or click chemistry such as …
Influence Of Polyunsaturated Fatty Acids On Intestinal Barrier Function During Colitis, Bianca Knoch, Warren C. Mcnabb, Nicole Roy
Influence Of Polyunsaturated Fatty Acids On Intestinal Barrier Function During Colitis, Bianca Knoch, Warren C. Mcnabb, Nicole Roy
Faculty of Science - Papers (Archive)
Tight junction proteins are important for intestinal homeostasis. They prevent paracellular transport of large molecules and maintain cell polarity. Impaired tight junction function leads to a more permeable intestinal epithelial barrier and therefore potentially increases disease risk. Limited information is available concerning the effects of food components on the intestinal barrier, particularly paracellular permeability and tight junction proteins. In vitro studies with intestinal epithelial cells and in vivo studies using animal models have demonstrated that dietary n-3 and n-6 polyunsaturated fatty acids (PUFAs), particularly n-3, can reduce intestinal inflammation and permeability. PUFAs can induce transcriptional regulators which may act in …
Importance Of Secondary Sources In The Atmospheric Budgets Of Formic And Acetic Acids, Fabien Paulot, Debra Wunch, John D. Crounse, G C Toon, Dylan B. Millet, Peter F. Decarlo, C Vigouroux, Nicholas M. Deutscher, G Gonzalez Abad, J Notholt, Thorsten Warneke, J Hannigan, Carsten Warneke, Joost A. De Gouw, Edward Dunlea, M. De Maziere, David W. Griffith, P Bernath, J L. Jimenez, Paul O. Wennberg
Importance Of Secondary Sources In The Atmospheric Budgets Of Formic And Acetic Acids, Fabien Paulot, Debra Wunch, John D. Crounse, G C Toon, Dylan B. Millet, Peter F. Decarlo, C Vigouroux, Nicholas M. Deutscher, G Gonzalez Abad, J Notholt, Thorsten Warneke, J Hannigan, Carsten Warneke, Joost A. De Gouw, Edward Dunlea, M. De Maziere, David W. Griffith, P Bernath, J L. Jimenez, Paul O. Wennberg
Faculty of Science - Papers (Archive)
We present a detailed budget of formic and acetic acids, two of the most abundant trace gases in the atmosphere. Our bottom-up estimate of the global source of formic and acetic acids are ∼1200 and ∼1400 Gmol/yr, dominated by photochemical oxidation of biogenic volatile organic compounds, in particular isoprene. Their sinks are dominated by wet and dry deposition. We use the GEOS-Chem chemical transport model to evaluate this budget against an extensive suite of measurements from ground, ship and satellite-based Fourier transform spectrometers, as well as from several aircraft campaigns over North America. The model captures the seasonality of formic …
Antimycobacterial Activity Of Cinnamate-Based Esters Of The Triterpenes Betulinic, Oleanolic And Ursolic Acids, Tanud Tanachatchairatana, John B. Bremner, Ratchanaporn Chokchaisiri, Apichart Suksamrarn
Antimycobacterial Activity Of Cinnamate-Based Esters Of The Triterpenes Betulinic, Oleanolic And Ursolic Acids, Tanud Tanachatchairatana, John B. Bremner, Ratchanaporn Chokchaisiri, Apichart Suksamrarn
Faculty of Science - Papers (Archive)
Betulinic acid, oleanolic acid and ursolic acid have been modified at the C-3 position to cinnamate-based esters and in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Ra has been determined. The results indicated that modification of the parent structures of betulinic acid, oleanolic acid and ursolic acid to the p-coumarate and, in the case of the latter two triterpenes, the ferulate ester analogues resulted in high antimycobacterial activity. Structure–activity relationships within the lupane, oleanane and ursane analogues and between these triterpenes are discussed.
Free Fatty Acids From The Crude Hexane Extract Of The Aerial Parts Of Heliotropium Indicum Linn. Growing In Phitsanulok, Thailand, Theeraphan Machan, Jurgen Korth, Boonsom Liawruangrath, Saisunee Liawruangrath, Apiwat Baramee, Stephen G. Pyne
Free Fatty Acids From The Crude Hexane Extract Of The Aerial Parts Of Heliotropium Indicum Linn. Growing In Phitsanulok, Thailand, Theeraphan Machan, Jurgen Korth, Boonsom Liawruangrath, Saisunee Liawruangrath, Apiwat Baramee, Stephen G. Pyne
Faculty of Science - Papers (Archive)
Sixteen free fatty acids from the crude hexane extract of the aerial parts of Heliotropium indicum Linn. growing in Phitsanulok, Thailand, have been identified after conversion to their methyl esters with boron trifluoride-methanol followed by quantification by GC-FID and identification by GC-MS analysis. They accounted for 95% of the chromatographable components, with 9,12-octadecadienoic acid, (39.7%), 9-octadecenoic acid (32.4%), hexadecanoic acid (14.2%) and octadecanoic acid (5.1%), as the major constituents. A small amount of 6,10,14-trimethyl-2-pentadecanone and 3,7,11,15-tetramethyl-2-hexadecen-1-ol as well as a homologous series of n-alkanes present at trace level and ranging from C25 to C31 was also found …
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
Effect Of Organic Solvents On The Separation Of Benzoic Acids By Capillary Electrophoresis, Young J. Lee, William E. Price, Margaret Sheil
Faculty of Science - Papers (Archive)
The effect of organic modifiers on the separation of a number of closely related isomeric benzoic acids by capillary electrophoresis is described. It is shown that while a single modifier concentration cannot help resolve the entire electropherogram, organic modifiers do significantly enhance the resolution of parts of the separation system by comparison with 40 mmol l-1 phosphate buffer. The effects on separation and retention times are discussed in terms of the effects on electroosmotic flow and the electrophoretic mobilities of the charged solutes. The effects were found to be modifier specific, although the trends were in the same direction (ie., …