Physical Sciences and Mathematics | Open Access Articles

N-Glycolisation Regulates Endothelial Lipase-Mediated Phospholipid Hydrolysis In Apoe- And Apoa-I-Containing High Density Lipoproteins, Danielle Skropeta, Daniel Rader, Weijun Jin, Monica Mcmahon, Kate Shearston, Philip Barter, Kristine Mcgrath, Kerry-Anne Rye, Chatri Settasatian, Daniela Caiazza

Danielle Skropeta

No abstract provided.

High-Yield Cell-Free Protein Synthesis For Site-Specific Incorporation Of Unnatural Amino Acids At Two Sites, Kiyoshi Ozawa, Karin V. Loscha, Kekini V. Kuppan, Choy Theng Loh, Nicholas E. Dixon, Gottfried Otting

Professor Nick E Dixon

Using aminoacyl-tRNA synthetase/suppressor tRNA pairs derived from Methanocaldococcus jannaschii, an Escherichia coli cell-free protein production system affords proteins with site-specifically incorporated unnatural amino acids (UAAs) in high yields through the use of optimized amber suppressor tRNACUA opt and optimization of reagent concentrations. The efficiency of the cell-free system allows the incorporation of trifluoromethyl-phenylalanine using a polyspecific synthetase evolved previously for p-cyanophenylalanine, and the incorporation of UAAs at two different sites of the same protein without any re-engineering of the E. coli cells used to make the cell-free extract.

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A High-Resolution Record Of Vegetation And Climate Through The Last Glacial Cycle From Caledonia Fen, Southeastern Highlands Of Australia, A Kershaw, G Mckenzie, N Porch, Richard Roberts, J Browne, H Heijnis, M Orr, Geraldine Jacobsen, P Newall

Richard G Roberts

A blocked tributary has provided a rare site of long-term sediment accumulation in montane southeastern Australia. This site has yielded a continuous, detailed pollen record through the last ca. 140 000 years and revealed marked vegetation and environmental changes at orbital to sub-millennial scales. Radiocarbon and optically stimulated luminescence (OSL, or optical) ages provide some chronological control for the last ca. 70 000 years. Most of the sediment is inorganic but with well preserved pollen that accumulated under unproductive and probably largely ice-covered lake conditions. The lake was surrounded by low-growing plants with an alpine character. Exceptions include three discrete …