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Plant Sciences

University of Kentucky

Perennial ryegrass

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Modelling The Effect Of A Variable Light Extinction Coefficient And Leaf Dispersion On Light Partitioning Between Species In A Grass-Clover Mixture, M Nassiri, A Elgersma Apr 2024

Modelling The Effect Of A Variable Light Extinction Coefficient And Leaf Dispersion On Light Partitioning Between Species In A Grass-Clover Mixture, M Nassiri, A Elgersma

IGC Proceedings (1997-2023)

Light partitioning in a grass-clover mixture was studied using a general competition model. The model assumes a fixed extinction coefficient (k) and no leaf dispersion. This was compared with modelling the effect of different k of both species over height as well as the effect of type of leaf dispersion. These new assumptions led to a better estimation of the light partitioning between both species. For grass the effect of variable k and leaf dispersion was similar and in both cases the total absorbed light was lower than under default conditions. For clover the new assumptions led to a higher …


Allocation Of Carbon-14 To Roots Of Different Ages In Perennial Ryegrass (Lolium Perenne L.), C Matthew, W D. Kemball Apr 2024

Allocation Of Carbon-14 To Roots Of Different Ages In Perennial Ryegrass (Lolium Perenne L.), C Matthew, W D. Kemball

IGC Proceedings (1997-2023)

The objective of this study was to provide information on how current photosynthate allocated to the root system in perennial ryegrass (Lolium perenne L.) is distributed among individual roots at differing nodal positions. Mature single ryegrass tillers were transplanted in September 1993 to glass house pots and three months later four of the plants were supplied with 14CO2. Individual roots from these radioactively-labeled plants were identified according to their nodal position on the tiller axis and amount of radiocarbon in each root quantified for root tip segments and for the remainder of the root axis. Similar plants were destructively …


Evaluating Cultivars Of Subterranean Clover In Monoculture Or With Perennial Ryegrass, K F.M. Reed, S G. Clark, W M. Feely Mar 2024

Evaluating Cultivars Of Subterranean Clover In Monoculture Or With Perennial Ryegrass, K F.M. Reed, S G. Clark, W M. Feely

IGC Proceedings (1997-2023)

Over four years, sowing perennial ryegrass (Lolium perenne) with a range of subterranean clover (“sub clover”, Trifolium subterraneum) cultivars was found to reduce the yield of clover per se by 31% and increase pasture yield by 11%. For one high-producing experimental variety, the presence of grass did not reduce clover yield (P<0.001). Clover yield in monoculture plots was poorly correlated with that measured in mixed swards (r = 0.44), but clover yield (± grass) was correlated with total herbage yield (r = 0.94). Clover seed yield was not reduced by the presence of grass. However, in year 2 - but not in the establishment year - grass reduced seed weight. Irrespective of grass treatment, the new late maturing cultivar, Leura outyielded the other commercial cultivars (P<0.05). Enfield and Enfield x Daliak crosses set the most seed and regenerated with a greater density than other cultivars (P<0.05) - apart from Leura. It was concluded that the winter-active, late maturing cultivar, Leura, will compete most successfully with perennial ryegrass.


Breeding Morphogenetic Traits To Match Genotypes To Their Utilization, L Hazard, M Betin, M Ghesquiere Mar 2024

Breeding Morphogenetic Traits To Match Genotypes To Their Utilization, L Hazard, M Betin, M Ghesquiere

IGC Proceedings (1997-2023)

A divergent selection for lamina length was done from natural populations of perennial ryegrass. Tested in a multi-site experiment in France, the short-leaved perennial ryegrass was more productive under simulated grazing and less productive under infrequent cutting than the long-leaved perennial ryegrass. Matching cultivars to their management is possible by selecting for morphogenetic traits. However, our results suggest the range of adaptation to different managements of perennial ryegrass cultivars could be extended by increasing their phenotypic plasticity.


Mitochondrial Genome Polymorphism In Lolium Perenne, Y Shimamoto, Y Tominaga, M Sato, T Mikami Mar 2024

Mitochondrial Genome Polymorphism In Lolium Perenne, Y Shimamoto, Y Tominaga, M Sato, T Mikami

IGC Proceedings (1997-2023)

The restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) of perennial ryegrass (Lolium perenne L.) were investigated to elucidate the genetic relatedness among the 128 cultivars including diploid and tetraploid. Many patterns of RFLPs were observed and allowed assigning of the cultivars into the main eight haplotypes of mitochondrial genome relatedness. The American cultivars were classified into haplotype I and VIII which were remote at the mitochondrial genome from each other, the European ones were distributed to all haplotypes and the tetraploid ones were mostly assigned into the haplotype V. The assessment of mtDNA RFLPs may be a …


Compared Productivity Of Local Ecotypes And Selected Cultivars Of Perennial Ryegrass (Lolium Perenne) In High Belgium, P Limbourg, Ph Lecomte Feb 2024

Compared Productivity Of Local Ecotypes And Selected Cultivars Of Perennial Ryegrass (Lolium Perenne) In High Belgium, P Limbourg, Ph Lecomte

IGC Proceedings (1997-2023)

The objective of the trials was to compare the productivity of local ecotypes of perennial ryegrass (Lolium perenne) issued from old permanent pastures of High Belgium to that of adapted cultivars. During 3 consecutive years 2 local ecotypes were compared, in pure seedling at 2 different levels of nitrogenous manure, to 2 cultivars frequently used in seed mixtures for pastures. Results obtained using a cutting cycle wich simulated grazing did not reveal significant differences in terms of forage production or feeding value. These results explain the weak increase in forage production recorded in pastures renovated by reseeding as …


Agricultural Performance Of Diverse Pastures Of Complementary Species And Monoculture Pastures Defoliated According To The Leaf Regrowth Stage Window Of Opportunity Criterion, B. A. Oliviera, I. F. López, L. M. Cranston, D. J. Donaghy, P. D. Kemp, J. Dörner Feb 2024

Agricultural Performance Of Diverse Pastures Of Complementary Species And Monoculture Pastures Defoliated According To The Leaf Regrowth Stage Window Of Opportunity Criterion, B. A. Oliviera, I. F. López, L. M. Cranston, D. J. Donaghy, P. D. Kemp, J. Dörner

IGC Proceedings (1997-2023)

In a diverse pasture of complementary species (DPCS), individual species fulfil agro-ecological functions that confer growth asynchrony and complementarity of ecosystem functions. These attributes provide yield consistency with a more even forage supply pattern across the year compared to monocultures. A common leaf regrowth stage window opportunity (LSWO) for the diverse species enables pasture defoliation that stimulates growth and persistence. The study assessed seasonal and annual growth traits of Lolium perenne (Lp), Bromus valdivianus (Bv) and Dactylis glomerata (Dg) as single grass species (Mono) sown with Trifolium repens (Tr) and as DPCS with the four species (Lp+Bv+Dg+Tr=Mix). The defoliation criteria …


Phyllochron And Growth Dynamic Of Lolium Perenne L. And Bromus Valdivianus Phil. As Affected By Soil Ph, Phosphorus And Nitrogen Restriction, Oscar A. Balocchi, M. F. Alonso, I. Calvache Jan 2024

Phyllochron And Growth Dynamic Of Lolium Perenne L. And Bromus Valdivianus Phil. As Affected By Soil Ph, Phosphorus And Nitrogen Restriction, Oscar A. Balocchi, M. F. Alonso, I. Calvache

IGC Proceedings (1997-2023)

Permanent grasslands are the main food for livestock production systems in Southern Chile. Therefore, it is essential to develop management tools that optimize the use of pastures. Among these tools, the phyllochron could be a better method to control grazing frequency. The aim of this study was to determine the effect of three levels of soil pH, phosphorus (P) and nitrogen (N) on the phyllochron and growth dynamic of Lolium perenne and Bromus valdivianus. Nine monophytic mini-swards of each species were established in 125 L containers for three different pH, P and N levels. For one year we measured: …


Perennial Ryegrass ‘Virazón’, A New Cultivar For Warm Temperate Areas In Uruguay And South America., Felix Gutiérrez, Javier Do Canto, R. Reyno, C. Rossi, A. Stewart, M. Carrere, F. Nolla Jan 2024

Perennial Ryegrass ‘Virazón’, A New Cultivar For Warm Temperate Areas In Uruguay And South America., Felix Gutiérrez, Javier Do Canto, R. Reyno, C. Rossi, A. Stewart, M. Carrere, F. Nolla

IGC Proceedings (1997-2023)

‘Virazón’ perennial ryegrass (Lolium perenne L.), a grazing-type tetraploid cultivar, was developed by the National Institute of Agricultural Research (INIA) in Uruguay as a part of the Joint Venture with PGG Wrightson Seeds and Grasslands Innovation Limited and commercially available since 2021. ‘Virazón’ was developed after 6 cycles of selection from crosses with cultivar Horizon from New Zealand based on climate adaptation, dry matter production, vigor, rust resistance, seed yield, and persistence under Uruguayan conditions. The aim was to obtain a perennial ryegrass with 3 years of consistent production. ‘Virazón’ was evaluated in forage yield trials in comparison with …


Plantain (Plantago Lanceolata L.) Growth Islimited Under Waterlogging, S. S. Wilson, Daniel J. Donaghy, D. J. Horne, Soledad Navarrete, P. D. Kemp Nov 2023

Plantain (Plantago Lanceolata L.) Growth Islimited Under Waterlogging, S. S. Wilson, Daniel J. Donaghy, D. J. Horne, Soledad Navarrete, P. D. Kemp

IGC Proceedings (1997-2023)

Plantain (Plantago lanceolata L.; PL) is becoming an increasingly important component of New Zealand dairy pastures because of its ability to reduce nitrogen losses to the environment. However, widespread suggestions of its poor persistence, have led to industry concern over its suitability for dairy systems. Anecdotal evidence suggests that PL does not cope well in waterlogged soil, yet there is little scientific literature relevant to this issue. Thus, the aim of this study was to investigate the impact of waterlogging stress on PL growth and survival. In a glasshouse, the performance of PL under waterlogging was evaluated against perennial …


Enhancing Water Use Efficiency On Irrigated Dairy Pastures With Nitrogen Fertiliser, Frank R. Mckenzie, J. L. Jacobs, G. N. Ward Aug 2023

Enhancing Water Use Efficiency On Irrigated Dairy Pastures With Nitrogen Fertiliser, Frank R. Mckenzie, J. L. Jacobs, G. N. Ward

IGC Proceedings (1997-2023)

Low summer rainfall in southwest Victoria, Australia, restricts pasture growth and limits milk production. One fifth of dairy farmers in the region have some capacity to irrigate during summer. Irrigated dairy pastures are relatively poor utilisers of water with water use efficiencies (WUE) of about 1 t DM/ML water (Ward et al. 1998). Using nitrogen (N) fertiliser may increase dry matter (DM) yields for a given amount of water. Data on N response efficiencies from irrigated pasture in southwest Victoria are lacking. Two experiments determined the potential of N fertiliser to maximise the conversion of irrigated water to pasture …


Responses Of Irrigated Pasture Nutritive Characteristics To Summer Nitrogen Fertiliser, Frank R. Mckenzie, J. L. Jacobs, G. N. Ward Aug 2023

Responses Of Irrigated Pasture Nutritive Characteristics To Summer Nitrogen Fertiliser, Frank R. Mckenzie, J. L. Jacobs, G. N. Ward

IGC Proceedings (1997-2023)

Low summer rainfall in southwest Victoria, Australia, restricts pasture growth and reduces pasture nutritive value thereby limiting potential milk production. One fifth of dairy farmers in the region have some capacity to irrigate during summer and nitrogen (N) fertiliser is used to enhance pasture dry matter (DM) yield. Data on the effects of N fertiliser on irrigated pasture nutritive characteristics during summer in southwest Victoria are lacking. Two experiments determined the potential of N fertiliser to improve pasture nutritive (crude protein, CP and metabolisable energy, ME) value during summer.


Yield Progress Of Perennial Ryegrass And Silage Maize - Genetic Gain Or Climate Change?, Antje Herrmann, A. Kornher, Friedhelm Taube Jun 2023

Yield Progress Of Perennial Ryegrass And Silage Maize - Genetic Gain Or Climate Change?, Antje Herrmann, A. Kornher, Friedhelm Taube

IGC Proceedings (1997-2023)

Gains in annual dry matter yield (DMY) from breeding achieved during the last decades are reported to range between 2.5 and 6% per decade for perennial ryegrass (Wilkins & Humphreys, 2003). In contrast, accelerated progress in improving DMY has been achieved for silage maize, varying between 8 and 13% per decade (Lauer et al., 2001). These gains are mainly attributed to (i) genetic yield potential increase, (ii) improved crop management and (iii) increased stress tolerance. The potential impact of climate change on yield progress, however, is disregarded in most studies. The objective of this study therefore was to quantify …


The Effect Of Extremes In Soil Moisture Content On Perennial Ryegrass Growth, A. Scott Laidlaw Jun 2023

The Effect Of Extremes In Soil Moisture Content On Perennial Ryegrass Growth, A. Scott Laidlaw

IGC Proceedings (1997-2023)

Seasonal distribution of rainfall in the UK and Ireland is predicted to become more variable (Sweeney, 2003). The problems of excessive soil moisture on grass utilisation and the effect of deficit in soil moisture on grass growth are well known. However the effect of excess rainfall on the growth of sown grass is less clear and is usually not taken into account in grass growth models. This study was carried out to investigate the potential impact of excess moisture on perennial ryegrass growth in the field and to investigate the relative effect of soil moisture varying from deficit to excess …


Water Soluble Carbohydrate Content Of Two Cultivars Of Perennial Ryegrass (Lolium Perenne) At Eight European Sites, Magnus A. Halling, A. C. Longland, S. D. Martens, L. Nesheim, P. O'Kiely Jun 2023

Water Soluble Carbohydrate Content Of Two Cultivars Of Perennial Ryegrass (Lolium Perenne) At Eight European Sites, Magnus A. Halling, A. C. Longland, S. D. Martens, L. Nesheim, P. O'Kiely

IGC Proceedings (1997-2023)

Grasses with high levels of WSC have been shown to enhance livestock production (Miller et al., 1999). This has led to the development of perennial ryegrass (PRG) cultivars that can accumulate high levels of WSC. The aim of this experiment was to determine if the genetic potential to accumulate high levels of WSC was expressed under varying conditions.


Agronomic Value Of Mixture Of Perennial Rye-Grass Cultivars: Preliminary Results, W. G. Do Nascimento, F. Surault, J. C. Emile, Christian Huyghe Jun 2023

Agronomic Value Of Mixture Of Perennial Rye-Grass Cultivars: Preliminary Results, W. G. Do Nascimento, F. Surault, J. C. Emile, Christian Huyghe

IGC Proceedings (1997-2023)

Mixtures of grass and legume species are commonly used in sown grasslands. Mixtures have been shown to be favourable for stable production over cycles and years due to a succession of species over time (Mosimann & Charles, 1996 ; Nie et al, 2004). However, little is known whether the genetic variation in pure stands has an influence on the agronomic value and its variation over seasons.


Black Medick - A Beneficial Companion Crop For Use In Organic Grass Production, R. Macháč, B. Cagaš Jun 2023

Black Medick - A Beneficial Companion Crop For Use In Organic Grass Production, R. Macháč, B. Cagaš

IGC Proceedings (1997-2023)

Organic farmers must use only organically produced seed for establishing new meadows and for, renovation and undersowing of old pastures, in accordance with EC regulations. Therefore an important and difficult goal is to obtain enough seed of grasses without the use of pesticides and inorganic fertilisers. The seed yield is closely related to the number of fertile tillers, which depends on adequate nitrogen in the soil. Growing grasses for seed with a legume, as a companion crop, is one possibility for providing a source of organic nitrogen. Aamlid (1999) claimed that growing timothy together with white clover or alsike clover …


The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan May 2023

The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan

IGC Proceedings (1997-2023)

In light of increasing environmental and economic pressure on agriculture to utilise resources more efficiently, protein feeding and its effects are fundamentally important. As grazed grass is the predominant feed in Irish dairy and beef cattle production systems, it is necessary to establish protein values for different grass varieties and cultivars fed. It is also important to investigate the extent of ruminal nitrogen (N) degradability for these grasses since this characteristic greatly influences environmentally damaging urinary N excretion.


Fibre Degradation Rate Of Perennial Ryegrass Varieties Measured Using Three Techniques: In Situ Nylon Bag, In Vivo Rumen Evacuation And In Vitro Gas Production, H. Z. Taweel, B. M. Tas, B. A. Williams, A. Elgersma, J. Dijkstra, S. Tamminga May 2023

Fibre Degradation Rate Of Perennial Ryegrass Varieties Measured Using Three Techniques: In Situ Nylon Bag, In Vivo Rumen Evacuation And In Vitro Gas Production, H. Z. Taweel, B. M. Tas, B. A. Williams, A. Elgersma, J. Dijkstra, S. Tamminga

IGC Proceedings (1997-2023)

In Western Europe, perennial ryegrass is the most widely used grass species for grazing cattle, because of its high productivity, palatability and nutritive value. However, the low dry matter intake (DMI) of perennial ryegrass pasture has been identified as a major factor limiting milk production of high producing dairy cows. Altering the chemical, physical and mechanical characteristics that contribute to its low DMI through grass breeding and the choice of variety may be a way forward in trying to maximise its DMI. This study aimed to examine whether perennial ryegrass varieties differ in their NDF degradation rates (kdNDF).


Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearon, F. E. A. Wilson May 2023

Perennial Ryegrass Variety Differences In Nutritive Value Characteristics, T. J. Gilliland, R. E. Agnew, A. M. Fearon, F. E. A. Wilson

IGC Proceedings (1997-2023)

Animal grazing performance at grass is predominately determined by herbage intake rates, with high yielding dairy cows requiring up to 20 kg/d DM within a limited grazing time (Gibb, 1998). Grass nutritional factors such as seasonal patterns in digestibility and water-soluble carbohydrate levels have been linked to animal productivity (Davies et al., 1991), while sward surface height, herbage mass, bulk density and green leaf mass have been shown to promote high grazing intake (Barrett et al., 2001). Furthermore, fatty acid profiles have been shown to improve the unsaturated fatty acid composition of milk, with potential human health benefits …


The Nutritional Value Of Cocksfoot (Dactylis Glomerata L.) And Perennial Ryegrass (Lolium Perenne L.) Under Leaf-Stage Based Defoliation Management, L. R. Turner, D. J. Donaghy, P. A. Lane May 2023

The Nutritional Value Of Cocksfoot (Dactylis Glomerata L.) And Perennial Ryegrass (Lolium Perenne L.) Under Leaf-Stage Based Defoliation Management, L. R. Turner, D. J. Donaghy, P. A. Lane

IGC Proceedings (1997-2023)

The perception that cocksfoot is of lower nutritional value and less productive than perennial ryegrass is largely the result of studies in which management was based on perennial ryegrass (to the detriment of cocksfoot) or involved defoliation of both species at the same time (Greenhalgh & Reid, 1969; Johnson & Thomson, 1996). Maintaining plants in a vegetative state through species-specific management is essential to retain the high quality and productivity of cocksfoot. Defoliation at or before 4-5 fully expanded live leaves per tiller is recommended as the ideal physiological regrowth stage for defoliation of cocksfoot, resulting in metabolisable energy (ME) …


Population Genetics Of Perennial Ryegrass (Lolium Perenne L.): Differentiation Of Pasture And Turf Cultivars, M. P. Dobrowolski, N. R. Bannan, R. C. Ponting, J. W. Forster, K. F. Smith Apr 2023

Population Genetics Of Perennial Ryegrass (Lolium Perenne L.): Differentiation Of Pasture And Turf Cultivars, M. P. Dobrowolski, N. R. Bannan, R. C. Ponting, J. W. Forster, K. F. Smith

IGC Proceedings (1997-2023)

Cultivar differentiation using molecular markers to assess genetic variation may be of value in obtaining or protecting plant breeders rights. A knowledge of genetic variation and how it is structured within perennial ryegrass (Lolium perenne L.) populations will also help us understand the consequences to fitness and adaptation when implementing molecular breeding strategies. In a study of the population genetic structure of a number of perennial ryegrass varieties we examined the cultivar differentiation potential of marker technology.


Mapping Water-Soluble Carbohydrate Content In Perennial Ryegrass, L. Turner, J. Gallagher, I. P. Armstead, A. Cairns, M. O. Humphreys Apr 2023

Mapping Water-Soluble Carbohydrate Content In Perennial Ryegrass, L. Turner, J. Gallagher, I. P. Armstead, A. Cairns, M. O. Humphreys

IGC Proceedings (1997-2023)

Perennial ryegrass (Lolium perenne L.) is the main species used in UK agriculture and shows considerable genetic variation for water-soluble carbohydrate (WSC) content (Humphreys, 1989, Turner et al., 2001, 2002). High-sugar grasses have already proved useful in UK livestock production (Miller et al., 2001), but can be unpredictable in the field. Increased understanding of carbon partitioning in ryegrass would benefit future breeding programmes.


Discovery, Isolation And Characterisation Of Promoters From Perennial Ryegrass (Lolium Perenne), A. Lidgett, N. Petrovska, J. Chalmers, N. Cummings, G. C. Spangenberg Apr 2023

Discovery, Isolation And Characterisation Of Promoters From Perennial Ryegrass (Lolium Perenne), A. Lidgett, N. Petrovska, J. Chalmers, N. Cummings, G. C. Spangenberg

IGC Proceedings (1997-2023)

The availability of a suite of promoters with a range of spatial, temporal and inducible expression patterns is of significant importance to enable targeted expression of genes of interest for molecular breeding of forage species. A range of resources and tools have been developed for promoter isolation and characterisation in perennial ryegrass (Lolium perenne L.) including genomic lambda and BAC libraries and a 15 K unigene microarray.


Agrobacterium Tumefaciens-Mediated Transformation Of Perennial Ryegrass (Lolium Perenne L.), H. Sato, M. Fujimori, Y. Mano, T. Kiyoshi, T. Takamizo Apr 2023

Agrobacterium Tumefaciens-Mediated Transformation Of Perennial Ryegrass (Lolium Perenne L.), H. Sato, M. Fujimori, Y. Mano, T. Kiyoshi, T. Takamizo

IGC Proceedings (1997-2023)

An Agrobacterium tumefaciens-mediated transformation method has several advantages. However, this method has no example of success in perennial ryegrass (Lolium perenne L.). Since Lolium species are outcrossing, one cultivar consists of many genotypes. Each genotype can show a different ability for callus formation and plant regeneration (Takahashi et al., 2004). Thus, it is important to select a good genotype for efficient and stable transformation. If the plant is maintained in vitro, we can perform transformation using calli induced from shoot tips of the same genotype at any time. Our objective is to confirm an A. tumefaciens …


Isolation And Characterisation Of Genes Encoding Ice Recrystallisation Inhibition Proteins (Irips) In The Cryophilic Antarctic Hair-Grass (Deschampsia Antarctica) And The Temperate Perennial Ryegrass (Lolium Perenne), U. P. John, R. M. Polotnianka, K. A. Sivakumaran, L. Mackin, M. J. Kuiper, J. P. Talbot, O. Chew, G. D. Nugent, N. O. I. Cogan, M. C. Drayton, J. W. Forster, G. E. Schrauf, G. C. Spangenberg Apr 2023

Isolation And Characterisation Of Genes Encoding Ice Recrystallisation Inhibition Proteins (Irips) In The Cryophilic Antarctic Hair-Grass (Deschampsia Antarctica) And The Temperate Perennial Ryegrass (Lolium Perenne), U. P. John, R. M. Polotnianka, K. A. Sivakumaran, L. Mackin, M. J. Kuiper, J. P. Talbot, O. Chew, G. D. Nugent, N. O. I. Cogan, M. C. Drayton, J. W. Forster, G. E. Schrauf, G. C. Spangenberg

IGC Proceedings (1997-2023)

Antarctic hairgrass (D. antarctica Desv.), the only grass species indigenous to Antarctica, has a well developed tolerance of freezing, strongly induced by cold-acclimation. In response to low temperatures D. antarctica exhibits recrystallisation inhibition (RI) activity, localised to the apoplasm, that prevents further growth of ice crystals following freezing.


Gene Discovery And Molecular Dissection Of Lignin Biosynthesis In Perennial Ryegrass (Lolium Perenne), A. Lidgett, M. Emmerling, R. Heath, R. Mcinnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E. K. Ong, K. F. Smith, A. Mouradov, G. C. Spangenberg Apr 2023

Gene Discovery And Molecular Dissection Of Lignin Biosynthesis In Perennial Ryegrass (Lolium Perenne), A. Lidgett, M. Emmerling, R. Heath, R. Mcinnes, D. Lynch, A. Bartkowski, K. Fulgueras, T. Sawbridge, E. K. Ong, K. F. Smith, A. Mouradov, G. C. Spangenberg

IGC Proceedings (1997-2023)

Lignification of plant cell walls has been identified as a major factor limiting forage digestibility. It limits the amount of digestible energy available to livestock, resulting in an incomplete utilisation of cellulose and hemicellulose by ruminant animals. Modification of the lignin profile of ryegrasses (Lolium spp.) and fescues (Festuca spp.) is undertaken through modulating the expression of genes encoding enzymes involved in the biosynthesis of monolignols.


A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg Mar 2023

A Proposal For An International Transcriptome Initiative For Forage And Turf: Microarray Tools For Expression Profiling In Ryegrass, Clover And Grass Endophytes, T. Webster, N. Nguyen, C. Rhodes, S. A. Felitti, R. Chapman, D. Edwards, G. C. Spangenberg

IGC Proceedings (1997-2023)

Knowledge of the expression pattern of genes provides a valuable insight into gene function and role in determining the observed heritable phenotype. High–density cDNA and oligonucleotide microarrays represent powerful tools for transcriptome analysis to gain an understanding of gene expression patterns for thousands of genes. Internationally coordinated efforts in transcriptome analyses and sharing of microarray resources will benefit the advancement of our understanding of gene function in forage and turf species.


Ryegrass Astra: A Web-Based Resource For Lolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards Mar 2023

Ryegrass Astra: A Web-Based Resource For Lolium Est Analysis, G. C. Spangenberg, T. Sawbridge, E. K. Ong, C. G. Love, T. A. Erwin, E. G. Logan, D. Edwards

IGC Proceedings (1997-2023)

Perennial ryegrass (Lolium perenne L.) is a major grass species of temperate pastoral agriculture.


Development And Testing Of Novel Chloroplast Markers For Perennial Ryegrass From De Novo Sequencing And In Silico Sequences, S. Mcgrath, T. R. Hodkinson, S. Barth Mar 2023

Development And Testing Of Novel Chloroplast Markers For Perennial Ryegrass From De Novo Sequencing And In Silico Sequences, S. Mcgrath, T. R. Hodkinson, S. Barth

IGC Proceedings (1997-2023)

Chloroplast DNA is uniparentally inherited and non-recombinant in Lolium perenne. These properties make the chloroplast genome a useful tool for studying inter- and intra- specific relationships. Previous genetic studies on L. perenne have used chloroplast sequence data. However, the relative lack of variation in the chloroplast genome limits its usefulness for analysis at the single individual level within a species. However, chloroplast SSR markers have recently been shown to have high levels of polymorphism (Provan et al., 2004). This is the first study to design and employ such markers for L. perenne. The objectives of this study …