Physical Sciences and Mathematics Commons^™

Probiotic Strain Stenotrophomonas Acidaminiphila Bj1 Degrades And Reduces Chlorothalonil Toxicity To Soil Enzymes, Microbial Communities And Plant Roots, Qingming Zhang, Muhammad Saleem, Caixia Wang

Plant and Soil Sciences Faculty Publications

Chlorothalonil, a non-systemic and broad-spectrum fungicide, is widely used to control the pathogens of agricultural plants. Although microbial degradation of chlorothalonil is known, we know little about the colonization and degradation capacity of these microbes in the natural and semi-natural soil environments. Therefore, we studied the colonization and detoxification potential of a chlorothalonil degrading Stenotrophomonas acidaminiphila probiotic strain BJ1 in the soil under green conditions. The results from polymerase chain reaction-denaturing gradient gel electrophoresis demonstrated that probiotic strain BJ1 successfully colonized the soil by competing with the native biota. Moreover, the bacterial inoculation stimulated some members of indigenous soil microbial …

Integrating Herbivore Population Dynamics Into A Global Land Biosphere Model: Plugging Animals Into The Earth System, Shree R. S. Dangal, Hanqin Tian, Chaoqun Lu, Wei Ren, Shufen Pan, Jia Yang, Nicola Di Cosmo, Amy Hessl

Plant and Soil Sciences Faculty Publications

Mammalian herbivores are an essential component of grassland and savanna ecosystems, and with feedbacks to the climate system. To date, the response and feedbacks of mammalian herbivores to changes in both abiotic and biotic factors are poorly quantified and not adequately represented in the current global land surface modeling framework. In this study, we coupled herbivore population dynamics in a global land model (the Dynamic Land Ecosystem Model, DLEM 3.0) to simulate populations of horses, cattle, sheep, and goats, and their responses to changes in multiple environmental factors at the site level across different continents during 1980–2010. Simulated results show …

Insights Into Land Plant Evolution Garnered From The Marchantia Polymorpha Genome, John L. Bowman, Takayuki Kohchi, Katsuyuki T. Yamato, Jerry Jenkins, Shengqiang Shu, Kimitsune Ishizaki, Shohei Yamaoka, Ryuichi Nishihama, Yasukazu Nakamura, Frédéric Berger, Catherine Adam, Shiori Sugamata Aki, Felix Althoff, Takashi Araki, Mario A. Arteaga-Vazquez, Sureshkumar Balasubrmanian, Kerrie Barry, Diane Bauer, Christian R. Boehm, Liam Briginshaw, Juan Caballero-Perez, Bruno Catarino, Feng Chen, Shota Chiyoda, Mansi Chovatia, Kevin M. Davies, Mihails Delmans, Taku Demura, Tom Dierschke, Liam Dolan, Tomokazu Kawashima

Plant and Soil Sciences Faculty Publications

The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant. We analyzed the genome of the liverwort Marchantia polymorpha, a member of a basal land plant lineage. Relative to charophycean algae, land plant genomes are characterized by genes encoding novel biochemical pathways, new phytohormone signaling pathways (notably auxin), …

Silver Engineered Nanomaterials And Ions Elicit Species-Specific O2 Consumption Responses In Plant Growth Promoting Rhizobacteria, Ricky W. Lewis, Jason M. Unrine, Paul M. Bertsch, David H. Mcnear Jr.

Plant and Soil Sciences Faculty Publications

Metal containing engineered nanomaterials (ENMs) are now commonly used in various industrial and commercial applications. Many of these materials can be transformed during waste water treatment and ultimately enter terrestrial ecosystems via agriculturally applied biosolids. It is unclear how agriculturally important soil microbes will be affected by exposure to environmentally relevant, sublethal concentrations of ENMs and their transformation products (i.e., ions, aggregates, etc.). A method was developed, which puts O₂ consumption responses in terms of viability, and tested by examining the toxic effects of Ag⁺, Zn²⁺, and Ni²⁺ ions on the plant growth promoting …

The Soybean Rfg1 Gene Restricts Nodulation By Sinorhizobium Fredii Usda193, Yinglun Fan, Jinge Liu, Shanhua Lyu, Qi Wang, Shengming Yang, Hongyan Zhu

Plant and Soil Sciences Faculty Publications

Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogen-fixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene. The Rfg1 allele encodes a member of the Toll-interleukin receptor/nucleotide-binding site/leucine-rich repeat class of …

Managing Nitrous Oxide Emissions In Agricultural Fields, Mark S. Coyne, Wei Ren

Plant and Soil Sciences Research Report

Agriculture is a major contributor to atmospheric nitrous oxide (N₂O) (Smith et al., 2014; Tian et al., 2015). Unfortunately, nitrous oxide destroys stratospheric ozone (O₃) which protects us from ultraviolet radiation (Cicerone, 1989) and it increases ground level O_3, whichis an air pollutant threatening human health and food production. Nitrous oxide is also 298 times more potent than an equivalent amount of carbon dioxide (CO₂) in terms of trapping and absorbing reflected solar radiation (Forster et al., 2007). Basic chemistry and physics assure us that increased levels of N₂O in the …

Fruit Weight Is Controlled By Cell Size Regulator Encoding A Novel Protein That Is Expressed In Maturing Tomato Fruits, Qi Mu, Zejun Huang, Manohar Chakrabarti, Eudald Illa-Berenguer, Xiaoxi Liu, Yanping Wang, Alexis Ramos, Esther Van Der Knaap

Plant and Soil Sciences Faculty Publications

Increases in fruit weight of cultivated vegetables and fruits accompanied the domestication of these crops. Here we report on the positional cloning of a quantitative trait locus (QTL) controlling fruit weight in tomato. The derived allele of Cell Size Regulator (CSR-D) increases fruit weight predominantly through enlargement of the pericarp areas. The expanded pericarp tissues result from increased mesocarp cell size and not from increased number of cell layers. The effect of CSR on fruit weight and cell size is found across different genetic backgrounds implying a consistent impact of the locus on the trait. In fruits, CSR …

Effects Of Cultivar And Maternal Environment On Seed Quality In Vicia Sativa, Rong Li, Lijun Chen, Yanpei Wu, Rui Zhang, Carol C. Baskin, Jerry M. Baskin, Xiaowen Hu

Biology Faculty Publications

Production of high quality seeds is of fundamental importance for successful crop production. However, knowledge of the effects of increased temperature resulting from global warming on seed quality of alpine species is limited. We investigated the effect of maternal environment on seed quality of three cultivars of the leguminous forage species Vicia sativa, giving particular attention to temperature. Plants of each cultivar were grown at 1700 and 3000 m a.s.l., and mass, germination, electrical conductivity (EC) of leakage and longevity were determined for mature seeds. Seeds of all three cultivars produced at the low elevation had a significantly lower …

Effects Of Climate Warming On Net Primary Productivity In China During 1961–2010, Fengxue Gu, Yuandong Zhang, Mei Huang, Bo Tao, Rui Guo, Changrong Yan

Plant and Soil Sciences Faculty Publications

The response of ecosystems to different magnitudes of climate warming and corresponding precipitation changes during the last few decades may provide an important reference for predicting the magnitude and trajectory of net primary productivity (NPP) in the future. In this study, a process-based ecosystem model, Carbon Exchange between Vegetation, Soil and Atmosphere (CEVSA), was used to investigate the response of NPP to warming at both national and subregional scales during 1961–2010. The results suggest that a 1.3°C increase in temperature stimulated the positive changing trend in NPP at national scale during the past 50 years. Regardless of the magnitude of …

Assessing Coastal Plain Risk Indices For Subsurface Phosphorus Loss, Amy L. Shober, Anthony R. Buda, Kathryn C. Turner, Nicole M. Fiorellino, A. Scott Andres, Joshua M. Mcgrath, J. Thomas Sims

Plant and Soil Sciences Faculty Publications

Phosphorus (P) Index evaluations are critical to advancing nutrient management planning in the United States. However, most assessments until now have focused on the risks of P losses in surface runoff. In artificially drained agroecosystems of the Atlantic Coastal Plain, subsurface flow is the predominant mode of P transport, but its representation in most P Indices is often inadequate. We explored methods to evaluate the subsurface P risk routines of five P Indices from Delaware, Maryland (two), Virginia, and North Carolina using available water quality and soils datasets. Relationships between subsurface P risk scores and published dissolved P loads in …

Conservation Farming And Changing Climate: More Beneficial Than Conventional Methods For Degraded Ugandan Soils, Drake N. Mubiru, Jalia Namakula, James Lwasa, Godfrey A. Otim, Joselyn Kashagama, Milly Nakafeero, William Nanyeenya, Mark S. Coyne

Plant and Soil Sciences Faculty Publications

The extent of land affected by degradation in Uganda ranges from 20% in relatively flat and vegetation-covered areas to 90% in the eastern and southwestern highlands. Land degradation has adversely affected smallholder agro-ecosystems including direct damage and loss of critical ecosystem services such as agricultural land/soil and biodiversity. This study evaluated the extent of bare grounds in Nakasongola, one of the districts in the Cattle Corridor of Uganda and the yield responses of maize (Zea mays) and common bean (Phaseolus vulgaris L.) to different tillage methods in the district. Bare ground was determined by a supervised multi-band …

Seedling Tolerance To Cotyledon Removal Varies With Seed Size: A Case Of Five Legume Species, Xiao Wen Hu, Rui Zhang, Yan Pei Wu, Carol C. Baskin

Biology Faculty Publications

It is generally accepted that seedlings from large seeds are more tolerant to defoliation than those from small seeds due to the additional metabolic reserves present in the large seeds. However, information on the effects of amount of seed reserves (cotyledon removal) from seedlings resulting from large vs. small seeds on seedling growth and long-term survival in the field is limited. Five legume species with different sizes of seeds were sown in the field and none, one, or both cotyledons removed 7 days after seedling emergence. Seedling biomass, relative growth rate (RGR) and survival were determined at different time. Cotyledon …

Divergence In Life History Traits Between Two Populations Of A Seed-Dimorphic Halophyte In Response To Soil Salinity, Fan Yang, Jerry M. Baskin, Carol C. Baskin, Xuejun Yang, Dechang Cao, Zhenying Huang

Biology Faculty Publications

Production of heteromorphic seeds is common in halophytes growing in arid environments with strong spatial and temporal heterogeneity. However, evidence for geographic variation (reflecting local adaptation) is almost nonexistent. Our primary aims were to compare the life history traits of two desert populations of this halophytic summer annual Suaeda corniculata subsp. mongolica and to investigate the phenotypic response of its plant and heteromorphic seeds to different levels of salt stress. Dimorphic seeds (F₁) of the halophyte S. corniculata collected from two distant populations (F₀) that differ in soil salinity were grown in a common environment under …

Global Land Carbon Sink Response To Temperature And Precipitation Varies With Enso Phase, Yuanyuan Fang, Anna M. Michalak, Christopher R. Schwalm, Deborah N. Huntzinger, Joseph A. Berry, Philippe Ciais, Shilong Piao, Benjamin Poulter, Joshua B. Fisher, Robert B. Cook, Daniel Hayes, Maoyi Huang, Akihiko Ito, Atul Jain, Huimin Lei, Chaoqun Lu, Jiafu Mao, Nicholas C. Parazoo, Shushi Peng, Daniel M. Ricciuto, Xiaoying Shi, Bo Tao, Hanqin Tian, Weile Wang, Yaxing Wei, Jia Yang

Plant and Soil Sciences Faculty Publications

Climate variability associated with the El Niño-Southern Oscillation (ENSO) and its consequent impacts on land carbon sink interannual variability have been used as a basis for investigating carbon cycle responses to climate variability more broadly, and to inform the sensitivity of the tropical carbon budget to climate change. Past studies have presented opposing views about whether temperature or precipitation is the primary factor driving the response of the land carbon sink to ENSO. Here, we show that the dominant driver varies with ENSO phase. Whereas tropical temperature explains sink dynamics following El Niño conditions (r TG,P = 0.59, p …

Noncanonical Alternative Polyadenylation Contributes To Gene Regulation In Response To Hypoxia, Laura De Lorenzo, Reed Sorenson, Julia Bailey-Serres, Arthur G. Hunt

Plant and Soil Sciences Faculty Publications

Stresses from various environmental challenges continually confront plants, and their responses are important for growth and survival. One molecular response to such challenges involves the alternative polyadenylation of mRNA. In plants, it is unclear how stress affects the production and fate of alternative mRNA isoforms. Using a genome-scale approach, we show that in Arabidopsis thaliana, hypoxia leads to increases in the number of mRNA isoforms with polyadenylated 3′ ends that map to 5′-untranslated regions (UTRs), introns, and protein-coding regions. RNAs with 3′ ends within protein-coding regions and introns were less stable than mRNAs that end at 3′-UTR poly(A) sites. …

Nutrient Addition Shifts Plant Community Composition Towards Earlier Flowering Species In Some Prairie Ecoregions In The U.S. Central Plains, Lori Biederman, Brent Mortensen, Philip Fay, Nicole Hagenah, Johannes Knops, Kimberly La Pierre, Ramesh Laungani, Eric Lind, Rebecca L. Mcculley, Sally Power, Eric Seabloom, Pedro Tognetti

Plant and Soil Sciences Faculty Publications

The distribution of flowering across the growing season is governed by each species’ evolutionary history and climatic variability. However, global change factors, such as eutrophication and invasion, can alter plant community composition and thus change the distribution of flowering across the growing season. We examined three ecoregions (tall-, mixed, and short-grass prairie) across the U.S. Central Plains to determine how nutrient (nitrogen (N), phosphorus, and potassium (+micronutrient)) addition alters the temporal patterns of plant flowering traits. We calculated total community flowering potential (FP) by distributing peak-season plant cover values across the growing season, allocating each species’ cover to only those …

Draft Nuclear Genome Sequence Of The Liquid Hydrocarbon–Accumulating Green Microalga Botryococcus Braunii Race B (Showa), Daniel R. Browne, Jerry Jenkins, Jeremy Schmutz, Shengqiang Shu, Kerrie Barry, Jane Grimwood, Jennifer Chiniquy, Aditi Sharma, Thomas Daniel Niehaus, Taylor L. Weiss, Andrew T. Koppisch, David T. Fox, Suraj Dhungana, Shigeru Okada, Joe Chappell, Timothy P. Devarenne

Plant and Soil Sciences Faculty Publications

Botryococcus braunii has long been known as a prodigious producer of liquid hydrocarbon oils that can be converted into combustion engine fuels. This draft genome for the B race of B. braunii will allow researchers to unravel important hydrocarbon biosynthetic pathways and identify possible regulatory networks controlling this unusual metabolism.

Oxidation Of Substituted Catechols At The Air-Water Interface: Production Of Carboxylic Acids, Quinones, And Polyphenols, Elizabeth A. Pillar, Marcelo I. Guzman

Chemistry Faculty Publications

Anthropogenic activities contribute benzene, toluene, and anisole to the environment, which in the atmosphere are converted into the respective phenols, cresols, and methoxyphenols by fast gas-phase reaction with hydroxyl radicals (HO(•)). Further processing of the latter species by HO(•) decreases their vapor pressure as a second hydroxyl group is incorporated to accelerate their oxidative aging at interfaces and in aqueous particles. This work shows how catechol, pyrogallol, 3-methylcatechol, 4-methylcatechol, and 3-methoxycatechol (all proxies for oxygenated aromatics derived from benzene, toluene, and anisole) react at the air-water interface with increasing O3(g) during τc ≈ 1 μs contact time and contrasts their …

Kentucky Alfalfa Awards History, Kentucky Alfalfa And Stored Forages Conference

Kentucky Alfalfa and Stored Forage Conference

The Kentucky Alfalfa Awards Program was initiated in 2000 at the 20th Anniversary of the Kentucky Alfalfa Conference. The Awards Program is funded annually from revenues generated each year for the Silent Auction during the Annual Conference.

Photo Highlights Of The 36th Kentucky Alfalfa And Stored Forages Conference, Kentucky Alfalfa And Stored Forages Conference

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

Alfalfa Conference Speakers From The Beginning, Kentucky Alfalfa And Stored Forages Conference

Kentucky Alfalfa and Stored Forage Conference

No abstract provided.

Save The Date! [Announcement], S. Ray Smith, Krista Lea

Kentucky Alfalfa and Stored Forage Conference

This is an announcement of upcoming events.

Selecting Summer Annual Varieties Using Yield And Digestibility, Christopher D. Teutsch

Kentucky Alfalfa and Stored Forage Conference

Although cool-season grasses can provide ample and high quality forage for grazing livestock in the spring and fall, forage growth during the summer months is often restricted by high temperatures (Figure 1). In contrast warm-season annual grasses are most productive during the summer months and do not reach peak growth until temperatures approach 90 degrees Fahrenheit. Summer annual grasses such as forage sorghum (Sorghum bicolor (L.) Moench), sudangrass (Sorghum bicolor (L.) Moench), sorghum-sudangrass hybrids, and pearl millet (Pennisetum americanum (L.) Leeke) can provide high quality summer grazing for ruminant livestock in many regions of the United States.

The Economics Of Hay Quality, Madeline L. Dant, Kenneth H. Burdine, Brandon Sears

Kentucky Alfalfa and Stored Forage Conference

Hay quality is often discussed when one considers the viability of cash hay operations. A great deal is known about how production impacts quality and how quality impacts animal performance. This knowledge certainly has economic implications, but the economics of hay quality has primarily been evaluated from the animal performance perspective. Analyzing the economics of hay quality from the cash hay producer’s prospective becomes more difficult due to data limitations.

There are approximately 2.5 million acres of hay produced in Kentucky annually. The vast majority of this hay is grass type hay that is produced and fed on beef cattle …

What Is New In Seed Coatings, Bill Talley

Kentucky Alfalfa and Stored Forage Conference

Seed Coatings started many centuries ago when the Chinese wrapped their rice seed in a mud ball when planting their rice fields. The ball would keep the seed from floating to the top when they flooded their fields. Seed treatment of Alfalfa first began with preinoculation of the seed to make it more convenient to plant. The farmer did not have to go through the process of inoculating the seed himself, and this was a great improvement at the time. In 1975 Ramsey Seed in California acquired technology from a New Zealand company to begin coating seed with a buildup …

Western Hay Production Vs. Eastern Hay Production, Tom Keene

Kentucky Alfalfa and Stored Forage Conference

Early in the history of mankind, man was nomadic and moved from location to location in order to gather and find sustenance for themselves as well as their livestock. At some point in time, man decided that rather than keep moving, they would establish a community in one location, usually near running water (or fresh water of some kind), and would begin to sustain themselves with production agricultural practices.

Planting and harvesting soon took the place of packing up and moving every time the current resources had been depleted. Each year a new crop would be planted; livestock also became …

Why Is Intake Reduced When Cattle Are Fed Tall Fescue?, James L. Klotz

Kentucky Alfalfa and Stored Forage Conference

A major problem for the cattle producer that utilizes tall fescue forage is the unrealized or reduced gains in body weight in growing animals. This result has been reproduced across numerous studies evaluating cattle performance on tall fescue (Hoveland et al., 1983; Boling, 1985; Schmidt et al., 1986; Goetsch et al., 1987). Cattle have gained from 30 to 100% less consuming toxic endophyte-infected tall fescue compared to consumption of an endophyte-free tall fescue (Paterson et al., 1995). This effect of reduced gain is a consequence of a fungal endophyte present within the grass that produces toxins called ergot alkaloids. Fungal …

Understanding And Improving Fermentation In Alfalfa And Grass Baleage, Dennis Hancock

Kentucky Alfalfa and Stored Forage Conference

The ancient Egyptian and Carthaginians are believed to be the first conserve forage by ensiling it in the absence of oxygen. Though the technique has been refined in the 3500 years since, the basic fermentation process has not changed. Populations of naturally-occurring bacteria on the plant surface can consume some of the readily available carbohydrates and produce organic acids. These organic acids lower the pH of the forage material and prevent fungal deterioration of the product. Fermentation has been used for millennia as a natural method for preserving food. Similar bacterial fermentation occurs when one makes yogurt, sour cream, or …

Hay Making Weather In Kentucky: How To Get Good Information, Matthew Dixon, Tom Priddy

Kentucky Alfalfa and Stored Forage Conference

The UK Agricultural Weather Center, housed within the Department of Biosystems and Agricultural Engineering, was developed in 1978. As part of the Cooperative Extension Service, the goal of the Ag Weather Center is to minimize weather and climate related surprise for Kentucky residents and their agricultural needs, ultimately for profitable and sustainable production. In doing so, numerous tools and models have been developed throughout the years to further help farmers and producers in management and production related decisions.

Foreword Of Kentucky Alfalfa And Stored Forages Conference [2017], S. Ray Smith, Krista Lea

Kentucky Alfalfa and Stored Forage Conference

This is the front matter of the proceedings.