Physical Sciences and Mathematics Commons^™

Growth Factor And Ultrasound-Assisted Bioreactor Synergism For Human Mesenchymal Stem Cell Chondrogenesis, Sanjukta Guha Thakurta, Gaurav Budhiraja, Anuradha Subramanian

Department of Chemical and Biomolecular Engineering: Faculty Publications

Ultrasound at 5.0 MHz was noted to be chondro-inductive, with improved SOX-9 gene and COL2A1 protein expression in constructs that allowed for cell-to-cell contact. To achieve tissue-engineered cartilage using macroporous scaffolds, it is hypothesized that a combination of ultrasound at 5.0 MHz and transforming growth factor-β3 induces human mesenchymal stem cell differentiation to chondrocytes. Expression of miR-145 was used as a metric to qualitatively assess the efficacy of human mesenchymal stem cell conversion. Our results suggest that in group 1 (no transforming growth factor-β3, no ultrasound), as anticipated, human mesenchymal stem cells were not efficiently differentiated into chondrocytes, judging by …