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Full-Text Articles in Physical Sciences and Mathematics
New Methods For Measuring Transient Interactions Between Proteins And Curved Membranes, Philip P. Cheney
New Methods For Measuring Transient Interactions Between Proteins And Curved Membranes, Philip P. Cheney
Electronic Theses and Dissertations
Variations in the physical deformation of the plasma membrane play a significant role in the sorting and behavior of the proteins that occupy it. Determining the interplay between membrane curvature and protein behavior required the development and thorough characterization of a model plasma membrane with well defined and localized regions of curvature. This model system consists of a fluid lipid bilayer that is supported by a dye-loaded polystyrene nanoparticle patterned glass substrate. As the physical deformation of the supported lipid bilayer is essential to our understanding of the behavior of the protein occupying the bilayer, extensive characterization of the structure …
Rapid Scan Electron Paramagnetic Resonance (Epr) And Digital Epr Development, Zhelin Yu
Rapid Scan Electron Paramagnetic Resonance (Epr) And Digital Epr Development, Zhelin Yu
Electronic Theses and Dissertations
Rapid scan electron paramagnetic resonance (EPR) was developed in the Eaton laboratory at the University of Denver. Applications of rapid scan to wider spectra, such as for immobilized nitroxides, spin-labeled proteins, irradiated tooth and fingernail samples were demonstrated in this dissertation. The scan width has been increased from 55 G to 160 G. The signal to noise (S/N) improvement for slowly tumbling spin-labeled protein samples that is provided by rapid scan EPR will be highly advantageous for biophysical studies. With substantial improvement in S/N by rapid scan, the dose estimation for irradiated tooth enamels became more reliable than …