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Full-Text Articles in Physical Sciences and Mathematics
Molecular Detection Of Campylobacter Spp. And Fecal Indicator Bacteria During The Northern Migration Of Sandhill Cranes (Grus Canadensis) At The Central Platte River, Jingrang Lu, Hodon Ryu, Jason R. Vogel, Jorge Santo Domingo, Nicholas J. Ashbolt
Molecular Detection Of Campylobacter Spp. And Fecal Indicator Bacteria During The Northern Migration Of Sandhill Cranes (Grus Canadensis) At The Central Platte River, Jingrang Lu, Hodon Ryu, Jason R. Vogel, Jorge Santo Domingo, Nicholas J. Ashbolt
United States Environmental Protection Agency: Staff Publications
The risk to human health of the annual sandhill crane (Grus canadensis) migration through Nebraska, which is thought to be a major source of fecal pollution of the central Platte River, is unknown. To better understand potential risks, the presence of Campylobacter species and three fecal indicator bacterial groups (Enterococcus spp., Escherichia coli, and Bacteroidetes) was assayed by PCR from crane excreta and water samples collected during their stopover at the Platte River, Nebraska, in 2010. Genus-specific PCR assays and sequence analyses identified Campylobacter jejuni as the predominant Campylobacter species in sandhill crane excreta. Campylobacter …
Comparison Of Pcr And Quantitative Real-Time Pcr Methods For The Characterization Of Ruminant And Cattle Fecal Pollution Sources, Meredith R. Raith, Catherine A. Kelty, John F. Griffith, Alexander Schriewer, Stefan Wuertz, Sophie Mieszkin, Michele Gourmelon, Georg H. Reischer, Andreas H. Farnleitner, Jared S. Ervin, Patricia A. Holden, Darcy L. Ebentier, Jennifer A. Jay, Dan Wang, Alexandria B. Boehm, Tiong Gim Aw, Joan B. Rose, E. Balleste, W.G. Meijer, Mano Sivaganesan, Orin C. Shanks
Comparison Of Pcr And Quantitative Real-Time Pcr Methods For The Characterization Of Ruminant And Cattle Fecal Pollution Sources, Meredith R. Raith, Catherine A. Kelty, John F. Griffith, Alexander Schriewer, Stefan Wuertz, Sophie Mieszkin, Michele Gourmelon, Georg H. Reischer, Andreas H. Farnleitner, Jared S. Ervin, Patricia A. Holden, Darcy L. Ebentier, Jennifer A. Jay, Dan Wang, Alexandria B. Boehm, Tiong Gim Aw, Joan B. Rose, E. Balleste, W.G. Meijer, Mano Sivaganesan, Orin C. Shanks
United States Environmental Protection Agency: Staff Publications
The State of California has mandated the preparation of a guidance document on the application of fecal source identification methods for recreational water quality management. California contains the fifth highest population of cattle in the United States, making the inclusion of cow-associated methods a logical choice. Because the performance of these methods has been shown to change based on geography and/or local animal feeding practices, laboratory comparisons are needed to determine which assays are best suited for implementation. We describe the performance characterization of two end-point PCR assays (CF128 and CF193) and five real-time quantitative PCR (qPCR) assays (Rum2Bac, BacR, …