Physical Sciences and Mathematics Commons^™

Comparison Of Individual And Pooled Sampling Methods For Detecting Bacterial Pathogens Of Fish, Sonia Mumford, Chris Patterson, Joy Evered, Ray Brunson, Jay Levine, Jim Winton

United States Geological Survey: Staff Publications

Examination of finfish populations for viral and bacterial pathogens is an important component of fish disease control programs worldwide. Two methods are commonly used for collecting tissue samples for bacteriological culture, the currently accepted standards for detection of bacterial fish pathogens. The method specified in the Office International des Epizooties Manual of Diagnostic Tests for Aquatic Animals permits combining renal and splenic tissues from as many as 5 fish into pooled samples. The American Fisheries Society (AFS) Blue Book/US Fish and Wildlife Service (USFWS) Inspection Manual specifies the use of a bacteriological loop for collecting samples from the kidney of …