Physical Sciences and Mathematics Commons^™

Isotopic (Δ13c, Δ14c) Analysis Of Organic Acids In Marine Samples Using Wet Chemical Oxidation, Susan Q. Lang, G. L. Früh-Green, S. M. Bernasconi, L. Wacker

Faculty Publications

We present a method for the isolation and off-line isotope analysis of formate and acetate in marine samples. Organic acids are separated by high performance liquid chromatography and collected in glass Exetainer® screw-capped vials that have been prespiked with an oxidant and flushed with helium. The vials are subsequently heated to convert the organic compounds to CO₂ for radiocarbon and δ¹³C analysis. Small aliquots are sampled for measurement of δ¹³C by isotope ratio mass spectrometry, whereas the majority of the CO₂ is saved for radiocarbon analysis by accelerator mass spectrometry using a gas ion …