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Full-Text Articles in Physical Sciences and Mathematics

Anti-Germinants As A New Strategy To Prevent Clostridium Difficile Infections, Amber Janece Howerton Dec 2012

Anti-Germinants As A New Strategy To Prevent Clostridium Difficile Infections, Amber Janece Howerton

UNLV Theses, Dissertations, Professional Papers, and Capstones

Clostridium difficileinfections (CDI) have emerged as a leading cause of hospital-associated complications. CDI is the major cause of antibiotic-related cases of diarrhea and nearly all cases of pseudomembranous colitis. The infective form of C. difficileis the spore, a dormant and hardy structure that forms under stress. Germination of C. difficile spores into toxin producing bacteria in the GI tract of susceptible patients is the first step in CDI establishment. Patient susceptibility occurs with a disruption of the natural gut microbiota by broad-spectrum antibiotics. Antibiotic treatments usually resolve CDI but refractory cases are on the rise. Of great concern is the …


Fecal Indicator Bacteria In Knitting Mill Creek With A Comparison Of Methods Used To Enumerate E. Coli, Manju Nagarajan Oct 2012

Fecal Indicator Bacteria In Knitting Mill Creek With A Comparison Of Methods Used To Enumerate E. Coli, Manju Nagarajan

OES Theses and Dissertations

This research seeks to evaluate the impact of an urban, multi-use neighborhood on the bacteriological quality of water in Knitting Mill Creek, a blind arm of the Lafayette River, a sub-estuary of the lower Chesapeake Bay. A principal objective was to determine the effects of rainfall, a surrogate for run-off, on water-column concentrations of fecal-indicator bacteria (FIB). I collected water samples weekly (September 2009-December 2010) and biweekly (January 2010-December 2011) at a storm-sewer outfall and a nearby marina and quantified their E. coli and enterococci concentrations using Colilert-18 and Enterolert, most-probable-number assays. In addition, I took monthly samples for these …


Effects Of Moisture Augmentation Of Municipal Solid Waste Through Addition Of Food Waste Or Wastewater Treatment Biosolids On Bio-Gas Formation For Power Generation, Jared Michael Gore Aug 2012

Effects Of Moisture Augmentation Of Municipal Solid Waste Through Addition Of Food Waste Or Wastewater Treatment Biosolids On Bio-Gas Formation For Power Generation, Jared Michael Gore

UNLV Theses, Dissertations, Professional Papers, and Capstones

An investigation into the effect of moisture augmentation by manipulation of food waste proportion or wastewater treatment plant biosolids proportion was undertaken to determine the effects on production of methane and other biogases from municipal solid waste (MSW). Laboratory microcosm experiments were performed to determine the effect of various proportions of influent waste streams on the production of biogas. Results indicated that moisture augmentation through the addition of food waste to MSW increases the overall bio-gas and hydrogen gas formed during fermentation. Moisture augmentation through addition of wastewater treatment bio-solids lead to inconclusive results. Addition of food waste to MSW …


Identification Of Persistent Long Range Interactions In GA95 And GB95 Through Thermal Unfolding Simulations, Milen Redai Tesfamariam Jul 2012

Identification Of Persistent Long Range Interactions In GA95 And GB95 Through Thermal Unfolding Simulations, Milen Redai Tesfamariam

Chemistry & Biochemistry Theses & Dissertations

For over five decades, different experiments have been performed to research how proteins attain their native three dimensional structures. However, the folding problem continues to be a puzzle in modern science. The design of two proteins that have maximal sequence identity but different folds and functions is one method that is being used to study the relationship between protein structure and amino acid sequence. In particular, mutant proteins of Streptococcus protein G, GA and GB, have 95% sequence identity and a 3a helix fold and β4/a fold, respectively. Molecular dynamics simulations of GA95 …


Synthesis Of Fluorogenic Substrates For The Enzymatic Characterization Of Rv0045c From M. Tuberculosis, Kelly Jo Mckenna May 2012

Synthesis Of Fluorogenic Substrates For The Enzymatic Characterization Of Rv0045c From M. Tuberculosis, Kelly Jo Mckenna

Undergraduate Honors Thesis Collection

Mycobacterium tuberculosis is the pathogenic bacterial agent commonly responsible for tuberculosis, or TB. Although treatment exists for the active form of tuberculosis, no method has been developed for eliminating M tuberculosis in its dormant state. One hypothesized method for the elimination of dormant TB is to develop an inhibitor specific for M tuberculosis esterases and lipases, as these esterases and lipases are essential to the survival of dormant TB infection. In this research, the substrate specificity of the Rv0045c esterase from M tuberculosis was studied due to the essential role of Rv0045c in TB metabolism and its dissimilarity to other …


The Use Of Diatoms To Infer Environmental Change Of The Lower White River, Southeastern Arkansas, Ruchi Bhattacharya May 2012

The Use Of Diatoms To Infer Environmental Change Of The Lower White River, Southeastern Arkansas, Ruchi Bhattacharya

Graduate Theses and Dissertations

This dissertation describes the limnology of the lower White River oxbow lakes in southeast Arkansas, inundated by the major 2008 spring flood. The flood water and sediment deposited in oxbow lakes were analyzed for water chemistry, sediment geochemistry and diatom assemblages and used as an analogue for past floods. The 2008 spring flood inundated and homogenized the entire floodplain; also represented by the predominantly planktonic diatom assemblage suggesting riverine input. Indicator species for eutrophication were also recorded consistent with the high nutrient content in the lakes. However, the variance in the surface diatom assemblage was best explained by the lake …


Evidence For Viral Infection In The Copepods Labidocera Aestiva And Acartia Tonsa In Tampa Bay, Florida, Darren Stephenson Dunlap Mar 2012

Evidence For Viral Infection In The Copepods Labidocera Aestiva And Acartia Tonsa In Tampa Bay, Florida, Darren Stephenson Dunlap

USF Tampa Graduate Theses and Dissertations

Mesozooplankton are of critical importance to marine food webs by transferring energy from the microbial food web to higher trophic levels and depositing energy to the deeper ocean layers through fecal deposition. While decades of research have shown that viruses have significant impacts in the oceans, and infect a wide range of organisms from bacteria to whales, there is still little known about the impacts of viruses on the mesozooplankton community. As copepods are the most abundant mesozooplankton group, this study sought to characterize the viruses present in natural populations of the calanoid copepods Acartia tonsa and Labidocera aestiva in …


Eukaryotic Microbes In The Deep Sea: Abundance, Diversity, And The Effect Of Pressure, Danielle Morgan-Smith Jan 2012

Eukaryotic Microbes In The Deep Sea: Abundance, Diversity, And The Effect Of Pressure, Danielle Morgan-Smith

OES Theses and Dissertations

The dark ocean is vast, high in pressure, cold, and scarce in resources, but has been shown to support a diverse and active microbial community wherever it is studied. Such studies, however, are scarce due to the difficulty of sampling at such depths, and are difficult to interpret due to compounding effects of pressure and temperature on physiology. Protists, functionally defined as the microbial portion of the domain Eukarya, are particularly neglected in studies of deep-sea microbiology. Here, I present three studies on microbial eukaryotes in the deep sea: first, a study of the abundance of microbial eukaryotes in the …


Grazing On Synechococcus Spp. By The Red-Tide Dinoflagellate Karenia Brevis: Implications For Bloom Dynamics In The Gulf Of Mexico, Leo A. Procise Jan 2012

Grazing On Synechococcus Spp. By The Red-Tide Dinoflagellate Karenia Brevis: Implications For Bloom Dynamics In The Gulf Of Mexico, Leo A. Procise

OES Theses and Dissertations

Karenia brevis, the toxic dinoflagellate responsible for massive red tides in the Gulf of Mexico (GOM), causes fish kills, shellfish poisoning, and acute respiratory irritation in humans. Bloom initiation and maintenance have been linked to the physical environment as well as various nutrient input mechanisms. To date, efforts to quantify nitrogen (N) sources fueling K. brevis blooms in the GOM have not included mixotrophic grazing although many dinoflagellates, including K. brevis, are known to be capable of mixotrophy. This dissertation reports field and laboratory results demonstrating that natural bloom populations and K. brevisisolates from the West Florida …