Physical Sciences and Mathematics Commons^™

The Effect Of Radiation And Repeated Sub-Culturing On Tgf-Β1 Signaling In Frtl-5 Cells, Cheryl G. Burrell

Loma Linda University Electronic Theses, Dissertations & Projects

From our ongoing in vitro studies using the Fisher Rat Thyroid cell line-5 (FRTL-5) we recorded accelerated growth, reduced follicularization and reduction in thyroxin release that occurred as the cells were repeatedly sub-cultured. We also recorded that these changes occurred earlier and more rapidly following radiation exposure. We determined that TGF-β1 production increased under both conditions. We hypothesized that alteration in the TGF-β1 signaling pathway contributed to the changes observed in the cellular properties of FRTL-5 cells. Our objective was to examine some of the players in the TGF-β1 signaling pathway to determine whether radiation and/or repeated subculturing promoted changes …

The Infection Dynamics Of Pav1 In The Caribbean Spiny Lobster Panulirus Argus, Caiwen Li

Dissertations, Theses, and Masters Projects

Panulirus argus Virus 1 (PaV1) is an emerging disease in Caribbean spiny lobster Panulirus argus. It is considered a threat to the lobster industry in the Florida Keys. In order to understand the infection dynamics of the PaV1 virus in the lobster host, a sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for diagnosis of PaV1 in lobsters. The lower limit of detection using the 110-bp DNA probe in a dot-blot hybridization for PaV1 was 10 pg of cloned DNA template and 10 ng of genomic DNA extracted from hemolymph of diseased spiny lobster. The probe specifically …

The Role Of Bacteria-Particle Interactions In Marine Snow Dynamics, Emily M. Yam

Dissertations, Theses, and Masters Projects

Marine snow aggregates are one of the primary vehicles for deep-sea carbon sequestration. Bacterial activity on marine snow affects both degradation and aggregation processes that determine the flux of carbon to depth, biogeochemical cycling, and microbial food webs. The microscale processes occurring on aggregates depend on specific interactions between bacteria and particles. In this thesis, I describe two such interactions which have larger scale implications on marine snow dynamics: (1) the effects of starvation on bacterial motility and colonization behaviors on marine aggregates and (2) interactions between bacteria and phytoplankton which could contribute to the production of TEP.

Current models …