Physical Sciences and Mathematics Commons^™

Developing A Microdialysis Sampling-Based Biofilm/Macrophage Co-Culture Model, Alda Diaz Perez

Graduate Theses and Dissertations

The host immune system and bacterial cells are known to interact during the human lifetime. Bacteria secrete a wide variety of signaling molecules, known as quorum sensing (QSC) molecules, that modulate the host immune system. While immune-biofilm interactions involve this chemical signaling network, the mechanisms through which this occurs are not well understood. This work aimed to develop a new method that can be used not only in vitro settings but also in vivo. The microdialysis sampling technique has widely been used in in vitro and in vivo settings in humans, mice, and rats for the collection of neuropeptides, cytokines, …

Using Soil Geospatial Properties And Environments To Explore Microbial Diversity, Sharon Faye Smith

Graduate Theses and Dissertations

Soil microorganisms help maintain nutrient cycling, control carbon sequestration, impact plant productivity, and influence several soil chemical and physical properties; yet, the processes that control the microbial composition of soil and how environmental changes may affect the composition and activity of these organisms at different scales remains a difficult and intriguing puzzle for soil scientists, ecologists, and modelers. Wetlands are endangered and important ecosystems that provide several services, which are directly linked to soil function. However, few wetland assessments consider the soil environment and microbial ecology. Linking soil microbial community composition and distribution patterns to soil physio-chemical properties would provide …

Phenotypic Switching Of Bacterial Cells In Extreme Environments, Sudip Nepal

Graduate Theses and Dissertations

A large number of terrestrial microbial lives thrive in extremes of environmental conditions, including extremes of pressure, temperature, salinity, pH, and a combination of them. For example, all the marine biomass thrive at high hydrostatic pressure depending on depth. The temperature in the ocean can be very high near the hydrothermal vents and salinity and pH depends on the composition of salt in the surrounding areas. On the surface, hot springs, lakes and geysers provide high temperature conditions, while many places are permafrost regions with subzero temperatures. There is an emerging body of work on the viability, genomics, and metagenomics …

Effects Of Light, Nutrients, And Salts On Microbial Biofilm Productivity And Detrital Processing In Aquatic Mesocosms, Bethanie Brooke Howard-Parker

Graduate Theses and Dissertations

Anthropogenic activities associated with urbanization, agriculture, and resource extraction continue to increase to support increasing needs of the growing population. These activities increase the amounts of pollutants entering freshwater streams and put aquatic ecosystems at structural and functional risk. Aquatic microbes play an important role in detrital processing in streams as a key linkage in moving carbon from detrital stocks into aquatic food webs. My research investigates the effects of light, nutrients, and salts on detrital microbes and decomposition in freshwaters using a mesocosm approach. In chapter one, I modified a current priming effect (PE) hypothesis model to include light …

The Release, Transport, And Utilization Of Phosphorus From Bed-Sediments: A Study Of A Eutrophic Littoral Cove On Beaver Lake In Northwest Arkansas, James A. Mccarty

Graduate Theses and Dissertations

Eutrophication of surface waters not only impacts the environment but also water treatment processes, the most significant of which is from the effects of algae. During peak algal growth in many southern U.S. reservoirs, inflows that bring nutrients are at an annual minimum, and phosphorus released from bed-sediments is trapped in the hypolimnion. Littoral areas, described as the most productive zone of the lake, may be a possible source of phosphorus that fuels algal growth in the reservoir. I studied an isolated shallow cove in the War Eagle Creek arm of Beaver Lake in Northwest Arkansas to measure, quantify, and …

Soil Microbial Diversity And Litter Decomposition Increase With Time Since Land Use Disturbance In Tropical Montane Forests Of Malaysian Borneo, Renee Sniegocki

Graduate Theses and Dissertations

Logging and forest conversion are occurring at alarming rates in the tropical forests of Southeast Asia. These disturbances alter soil chemistry, microbial diversity, and disrupt carbon cycling through shifts in litter decomposition. Direct links between microbial diversity and soil properties such as pH are well established; however, the indirect impacts of logging and forest conversion on microbial diversity and litter decomposition are poorly understood. We used surface (5 cm) soil to assess soil physicochemical properties, next-generation DNA sequencing to assess soil microbial diversity, and standardized litterbags to assess litter decomposition stabilization at five sites along a land use disturbance gradient …

Comparative Microbial Community Dynamics In A Karst Aquifer System And Proximal Surface Stream In Northwest Arkansas, Josue Rodriguez

Graduate Theses and Dissertations

Northwest Arkansas has well-developed karst systems, with numerous sinking streams and springs. Karst conduits make it easy for contaminants to enter groundwater systems, degrading the water quality and destroying fragile karst ecosystems. With the increase of urbanization, potential threats in the form of fecal contamination may prove to be more of a problem. The purpose of this research is to compare the difference between microbial communities within two different settings, a karst aquifer and a surface stream. The microbial communities within Blowing Spring Cave and Little Sugar Creek were detected and identified in water and sediment samples. Samples were also …

Fungi Of Forests: Examining The Diversity Of Root-Associated Fungi And Their Responses To Acid Deposition, Donald Jay Nelsen

Graduate Theses and Dissertations

Global importance of forests is difficult to overestimate, given their role in oxygen production, ecological roles in nutrient cycling and supporting numerous living species, and economic value for industry and as recreational zones. Fitness of the forest-forming trees strongly depends on microbial communities associated with tree roots. In particular, fungi impact tree fitness: mycorrhizal species provide water and nutrients for the trees in exchange for C, endophytic fungi play key roles in host defense against pathogenic organisms, and saprotrophic fungi decompose dead organic matter and facilitate nutrient cycling. In addition, pathogenic fungal species strongly affect forest fitness. Despite their importance, …

Growth And Survivability Of Microorganisms At Martian Temperatures And Pressures, Rebecca Lynne Mickol

Graduate Theses and Dissertations

The discovery of methane in the martian atmosphere via numerous ground- and space-based sources has prompted the study of methanogens as models for life on Mars. Methanogens are microorganisms within the domain Archaea, many of which utilize carbon dioxide (CO2) and hydrogen to produce methane. The non-photosynthetic nature of methanogens indicates that they could exist in sub-surface environments, protected from harmful UV and ionizing radiation on the surface of Mars. These organisms also do not require organics, which are sparse on the planet.

Additionally, the wide variety of environments we find life in on Earth, as well as evidence for …

Dissemination And Persistence Of Plasmid Located, Integron Associated Antibiotic Resistant Genes In Wastewater Treatment Plant Effluent And Stream Water Bacteria, _ Suhartono

Graduate Theses and Dissertations

Transmissible plasmid-mediated integrons play important role in the persistence and dissemination of antibiotic resistance throughout the environment. Plasmids from 139 multi-antibiotic resistant Escherichia coli recovered from wastewater treatment plant effluent and upstream and downstream receiving stream water in Northwest Arkansas were extracted and profiled. Genes of class 1 and class 2 integrase (intI), mobilization (mob), sulfamethoxazole resistance (sul), and trimethoprim resistance (dfr) were detected using PCR and confirmed through DNA sequencing. Plasmids from almost half of the isolates (47%) were transmissible with mobF12 gene as the most frequently detected mobilization gene. Plasmid-borne class 1 with and without class 2 integrons …

The Functional And Distributional Ecology Of Mycetozoans Under Changing Edaphic And Climatic Dynamics, Geoffrey Lloyd Zahn

Graduate Theses and Dissertations

Investigations into the distribution and ecosystem functions of fruiting amoebae revealed that local-scale environmental conditions can largely explain broad biogeographical patterns in species assemblage, the way in which amoeboid predators shape bacterial communities and how this top-down influence may affect global biogeochemical processes in a changing climate. The distribution and assemblage of protosteloid amoebae on the islands of New Zealand and Hawaii did not yield any expected patterns of island biogeography, and conformed to other global regions studied. The strongest predictor of species richness in a given region was sampling effort and these species do not appear to have any …

Methane Emissions From Direct-Seeded, Delayed-Flood Rice Grown On A Clay Soil, Alden Daniel Smartt

Graduate Theses and Dissertations

Due to the production of methane (CH4) under flooded-soil conditions, rice (Oryza sativa L.) cultivation is a major contributor to agricultural CH4 emissions. Studies examining CH4 emissions from rice have only recently been initiated in Arkansas and no data have been collected from rice produced on clay soils in Arkansas. Therefore, research was conducted in 2012 and 2013 at the Northeast Research and Extension Center in Keiser, Arkansas to examine the factors affecting CH4 emissions from rice produced on a Sharkey clay (very-fine, smectitic, thermic Chromic Epiaquerts). The objectives of this study were to determine: 1) the effect of vegetation …

Bacterially-Mediated Formation Of Rock Coatings In Kärkevagge, Swedish Lapland: A Mineralogical And Micro-Environmental Analog For Mars, Cassandra L. Marnocha

Graduate Theses and Dissertations

The search for past or present life on Mars is, for now, limited to surface environments. An often neglected surface environment that could have served as an abode for life and could presently preserve evidence of that life is that of rock coatings. Rock coatings are mineral accretions on rock surfaces. On Earth, they are widespread and occur with considerable chemical diversity. There is growing evidence for a biotic role in their formation on Earth, particularly with respect to rock varnish. As a result, rock varnish has become a target of astrobiological interest on Mars, where varnish-like coatings have been …

Litter Conditioning Is Differentially Affected By Leaf Species, Phosphorus Enrichment, And Light Availability, Erin E. Scott

Graduate Theses and Dissertations

Anthropogenic enrichment of nitrogen and phosphorus is one of the most pervasive and detrimental threats to aquatic ecosystems worldwide. In streams that rely on allochthonous basal food resources, such as leaves, nutrient pollution can result in altered food quality and decreased carbon (C) standing stocks. However, the magnitude and mechanisms of this change in quality are poorly understood. Laboratory microcosm studies were conducted to 1) quantify the response of litter C:P to a gradient of phosphorus (P) enrichment (0, 0.05, and 0.5 mg SRP/L) across leaf species with variable levels of degradability (sugar maple and oak), and 2) quantify the …

The Use Of Diatoms To Infer Environmental Change Of The Lower White River, Southeastern Arkansas, Ruchi Bhattacharya

Graduate Theses and Dissertations

This dissertation describes the limnology of the lower White River oxbow lakes in southeast Arkansas, inundated by the major 2008 spring flood. The flood water and sediment deposited in oxbow lakes were analyzed for water chemistry, sediment geochemistry and diatom assemblages and used as an analogue for past floods. The 2008 spring flood inundated and homogenized the entire floodplain; also represented by the predominantly planktonic diatom assemblage suggesting riverine input. Indicator species for eutrophication were also recorded consistent with the high nutrient content in the lakes. However, the variance in the surface diatom assemblage was best explained by the lake …