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Microbiology

William & Mary

Biological Sciences Peer-Reviewed Articles

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Full-Text Articles in Physical Sciences and Mathematics

Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin Sep 2020

Trace Metal Availability Affects Greenhouse Gas Emissions And Microbial Functional Group Abundance In Freshwater Wetland Sediments, George Giannopoulos, Katherine R. Hartop, Bonnie L. Brown, Bongkeun Song, Lars Elsgaard, Rima B. Franklin

VIMS Articles

We investigated the effects of trace metal additions on microbial nitrogen (N) and carbon (C) cycling using freshwater wetland sediment microcosms amended with micromolar concentrations of copper (Cu), molybdenum (Mo), iron (Fe), and all combinations thereof. In addition to monitoring inorganic N transformations (NO3, NO2, N2O, NH4+) and carbon mineralization (CO2, CH4), we tracked changes in functional gene abundance associated with denitrification (nirS, nirK, nosZ), dissimilatory nitrate reduction to ammonium (DNRA; nrfA), and methanogenesis (mcrA). With regards to …


Changes In Bacterioplankton Metabolic Capabilities Along A Salinity Gradient In The York River Estuary, Virginia, Usa, G. E. Schultz, H. W. Ducklow Sep 2000

Changes In Bacterioplankton Metabolic Capabilities Along A Salinity Gradient In The York River Estuary, Virginia, Usa, G. E. Schultz, H. W. Ducklow

VIMS Articles

Changes in metabolic capabilities of bacterial communities along the estuarine salinity gradient may affect the extent of organic matter processing and bacterial growth and accumulation during transit through the system; As part of a larger study of estuarine microbial processes, we attempted to quantify differences in bacterial community structure using Biolog plates. Biolog GN plates (Biolog, Inc., Hayward, CA, USA) were used to determine differences in bacterioplankton community metabolic potential. Biolog GN microplates are 96-well microtiter plates in which each well contains an individual carbon source as well the redox dye tetrazolium violet. As bacteria grow and oxidize each substrate, …


Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell Jan 1999

Estimation Of Bacterial Respiration And Growth Efficiency In The Ross Sea, Antarctica, C. A. Carlson, N. R. Bates, H. W. Ducklow, D. A. Hansell

VIMS Articles

Seawater cultures were conducted in large volume (36 l) gas impermeable tri-laminate bags for the purpose of empirically deriving bacterial growth efficiency (BGE) and carbon conversion factors (CCF) in the south central Ross Sea. This experimental design allowed for concomitant measurements of metabolic reactants (loss of total and dissolved organic carbon [TOC and DOG]) and products (gain of total carbon dioxide [TCO2] and bacterial biomass) to be made from a single incubation vessel. Some previous studies have relied on proxy measurements (e.g. O-2, H-3-thymidine incorporation and cell abundance) to determine BGE and CCF rather than direct carbon measurements. Our experimental …


Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr. Jan 1999

Bacterial Growth In Experimental Plankton Assemblages And Seawater Cultures From The Phaeocystis Antarctica Bloom In The Ross Sea, Antarctica, H. W. Ducklow, C. Carlson, Walker O. Smith Jr.

VIMS Articles

A series of seawater culture experiments was carried out during the Phaeocystis antarctica bloom in the Ross Sea polynya (76.5 degrees S, 180 degrees W; November to December 1994 and December 1995 to January 1996) to examine bacterioplankton growth and derive empirical factors for estimating bacterial production rates. Bacterial growth was exponential over 3 to 10 d in all experiments, at rates of ca 0.1 to 0.7 d(-1), even in persistently cold waters (-2 to + 1 degrees C). Growth rates were lower in the early part of the bloom (early to mid-November) and highest during the period of peak …