Physical Sciences and Mathematics Commons^™

A Snapshot Of Ancient Microbial Life: Microorganisms And Organic Compounds In Primary Fluid Inclusions In Bedded Halite, Neoproterozoic Browne Formation Of Central Australia, Sara I. Schreder-Gomes

Graduate Theses, Dissertations, and Problem Reports

Modern shallow brine environments contain an abundance of microorganisms. Microorganisms, mineral crystals, and air bubbles can be trapped with those brines in primary fluid inclusions in bedded halite as it grows in shallow saline surface waters. Primary fluid inclusions and their contents can be preserved, unaltered, for millions of years.

The ~830 million-year-old Browne Formation of central Australia contains intervals of bedded halite with unaltered primary fluid inclusions. Intervals of bedded halite were sampled in the Empress 1A core from ~1480 m – 1520 m depths. Here, I used petrography to describe suspect microorganisms and organic compounds that were trapped …

Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust

Graduate Theses, Dissertations, and Problem Reports

Ergot alkaloids are fungal tryptophan derived toxins which affect mammalian circulation and neurotransmission. These compounds are biosynthesized by a conserved genetic pathway, known as the ergot alkaloid synthesis (EAS) pathway by fungi belonging to the ascomycete families Trichocomaceae and Clavicipitaceae. Several Ipomoea species and related plants in the morning glory family harbor vertically transmitted symbiotic fungi in the genus Periglandula, also members of Clavicipitaceae, that produce ergot alkaloids. Metabolomic analysis of seeds identified a previously uncharacterized glycoside form of the pharmaceutically important ergot alkaloid, ergonovine. Several species belonging to the fungal genus Metarhizium have recently been shown to have the …

Microbial Responses To Unconventional Oil And Gas Development May Alter Ecosystem Function In Headwater Streams, Rachel Michaels

Graduate Theses, Dissertations, and Problem Reports

The demand for natural gas, and the need for efficient extraction, has led to the development of unconventional oil and natural gas (UOG) techniques. Due to the novelty of UOG, the potential impacts to freshwater ecosystems are not fully understood. We used a dual pronged approach to study the effects of UOG development on microbial biodiversity and function via a laboratory microcosm experiment and a survey study of streams with and without UOG development within their watersheds. The laboratory microcosm study simulated stream contamination with produced water, a byproduct of UOG operation, using sediment collected from one high water-quality stream …