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Full-Text Articles in Physical Sciences and Mathematics
New Technologies For Glycomic Analysis: Toward A Systematic Understanding Of The Glycome, John Rakus, Lara Mahal
New Technologies For Glycomic Analysis: Toward A Systematic Understanding Of The Glycome, John Rakus, Lara Mahal
John F. Rakus
Carbohydrates are the most difficult class of biological molecules to study by high-throughput methods owing to the chemical similarities between the constituent monosaccharide building blocks, template-less biosynthesis, and the lack of clearly identifiable consensus sequences for the glycan modification of cohorts of glycoproteins. These molecules are crucial for a wide variety of cellular processes ranging from cell-cell communication to immunity, and they are altered in disease states such as cancer and inflammation. Thus, there has been a dedicated effort to develop glycan analysis into a high-throughput analytical field termed glycomics. Herein we highlight major advances in applying separation, mass spectrometry, …
Mir-30b/30d Regulation Of Galnac Transferases Enhances Invasion And Immunosuppression During Metastasis, Avital Gaziel-Sovran, Miguel Segura, Raffaella Di Micco, Mary Collins, Douglas Hanniford, Eleazar De Miera, John Rakus, John Dankert, Shulian Shang, Robert Kerbel, Nina Bhardwaj, Yongzhao Shao, Farbod Darvishian, Jiri Zavadil, Adrian Erlebacher, Lara Mahal, Iman Osman, Eva Hernando
Mir-30b/30d Regulation Of Galnac Transferases Enhances Invasion And Immunosuppression During Metastasis, Avital Gaziel-Sovran, Miguel Segura, Raffaella Di Micco, Mary Collins, Douglas Hanniford, Eleazar De Miera, John Rakus, John Dankert, Shulian Shang, Robert Kerbel, Nina Bhardwaj, Yongzhao Shao, Farbod Darvishian, Jiri Zavadil, Adrian Erlebacher, Lara Mahal, Iman Osman, Eva Hernando
John F. Rakus
To metastasize, a tumor cell must acquire abilities such as the capacity to colonize new tissue and evade immune surveillance. Recent evidence suggests that microRNAs can promote the evolution of malignant behaviors by regulating multiple targets. We performed a microRNA analysis of human melanoma, a highly invasive cancer, and found that miR-30b/30d upregulation correlates with stage, metastatic potential, shorter time to recurrence, and reduced overall survival. Ectopic expression of miR-30b/30d promoted the metastatic behavior of melanoma cells by directly targeting the GalNAc transferase GALNT7, resulted in increased synthesis of the immunosuppressive cytokine IL-10, and reduced immune cell activation and recruitment. …