Physical Sciences and Mathematics Commons^™

The Electrochemical Detection Of Interleukin-8, Cancer Biomarker, Based On A Gold Nanoparticle Platform And Its Political Implications, Jaimee Doucette

Pell Scholars and Senior Theses

Herein we report on an ultrasensitive immunosensor based on glutathione protected gold nanoparticle (GSH-AuNP) for the electrochemical detection of interleukin 8 (IL-8), cancer biomarker in calf serum and proof of concept IL-8 detection in HNSCC cells. GSH-AuNP were bioconjugated to the primary antibodies (Ab1) and used to capture human IL-8 in a sandwich electrochemical immunoassay coupled to horseradish peroxidase enzyme labels. Using the optimized concentrations of the primary (Ab1) and secondary antibodies (Ab2), two sensor approaches were used to measure ultra low (≤ 500 fg mL-1) and elevated levels of IL-8. Biotinylated Ab2 bound to streptavidin HRP with 14-16 labels …