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Development And Annotation Of Perennial Triticeae Ests And Ssr Markers., B.S. Bushman, S R Larson, I W Mott, P F Cliften, R R Wang, N J Chatterton, A G Hernandez, S Ali, R W Kim, Jyothi Thimmapuram, G Gong, L Liu, M A Mikel

Cyber Center Publications

Triticeae contains hundreds of species of both annual and perennial types. Although substantial genomic tools are available for annual Triticeae cereals such as wheat and barley, the perennial Triticeae lack sufficient genomic resources for genetic mapping or diversity research. To increase the amount of sequence information available in the perennial Triticeae, three expressed sequence tag (EST) libraries were developed and annotated for Pseudoroegneria spicata, a mixture of both Elymus wawawaiensis and E. lanceolatus, and a Leymus cinereus x L. triticoides interspecific hybrid. The ESTs were combined into unigene sets of 8 780 unigenes for P. spicata, 11 281 unigenes for …