Physical Sciences and Mathematics Commons^™

A Shortcut For Multiple Testing On The Directed Acyclic Graph Of Gene Ontology, Garrett Saunders, John R. Stevens, S. Clay Isom

Mathematics and Statistics Faculty Publications

Background: Gene set testing has become an important analysis technique in high throughput microarray and next generation sequencing studies for uncovering patterns of differential expression of various biological processes. Often, the large number of gene sets that are tested simultaneously require some sort of multiplicity correction to account for the multiplicity effect. This work provides a substantial computational improvement to an existing familywise error rate controlling multiplicity approach (the Focus Level method) for gene set testing in high throughput microarray and next generation sequencing studies using Gene Ontology graphs, which we call the Short Focus Level.

Results: The Short Focus …