Physical Sciences and Mathematics Commons^™

Sequencing Of 15 622 Gene-Bearing Bacs Clarifies The Gene-Dense Regions Of The Barley Genome, María Muñoz-Amatriaín, Stefano Lonardi, Mingcheng Luo, Kavitha Madishetty, Jan T. Svensson, Matthew J. Moscou, Steve Wanamaker, Tao Jiang, Andris Kleinhofs, Gary J. Muehlbauer, Roger P. Wise, Nils Stein, Shane Ma, Edmundo Rodriguez, Dave Kudrna, Prasanna R. Bhat, Shiaoman Chao, Pascal Condamine, Shane Heinen, Josh Resnik, Rod Wing, Heather N. Witt, Matthew Alpert, Marco Beccuti, Serdar Bozdag, Francesca Cordero, Hamid Mirebrahim, Rachid Ounit, Yonghui Wu, Frank You, Jie Zheng, Hana Simková, Jaroslav Dolezel, Jane Grimwood, Jeremy Schmutz, Denisa Duma, Lothar Altschmied, Tom Blake, Phil Bregitzer, Laurel Cooper, Muharrem Dilbirligi, Anders Falk, Leila Feiz, Andreas Graner, Perry Gustafson, Patrick M. Hayes, Peggy Lemaux, Jafar Mammadov, Timothy J. Close

Mathematics, Statistics and Computer Science Faculty Research and Publications

Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic …