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Full-Text Articles in Physical Sciences and Mathematics
Synthesis And Applications Of Covalent Protein-Dna Conjugates, Patrick M. Schaeffer, Nicholas E. Dixon
Synthesis And Applications Of Covalent Protein-Dna Conjugates, Patrick M. Schaeffer, Nicholas E. Dixon
Faculty of Science - Papers (Archive)
Synthetic protein-DNA conjugates are valuable tools with applications in fields including nanobiotechnology, bioanalytical chemistry, and molecular diagnostics, and various synthetic methods for their production have been developed during the past three decades. The present article reviews current methodologies for the synthesis of covalent protein-DNA conjugates with particular focus on the regiospecificity and stoichiometry of these reactions.
Glutarate And N-Acetyl-L-Glutamate Buffers For Cell-Free Synthesis Of Selectively 15n-Labelled Proteins, X Jia, Kiyoshi Ozawa, K Loscha, G Otting
Glutarate And N-Acetyl-L-Glutamate Buffers For Cell-Free Synthesis Of Selectively 15n-Labelled Proteins, X Jia, Kiyoshi Ozawa, K Loscha, G Otting
Faculty of Science - Papers (Archive)
Cell-free protein synthesis provides rapid and economical access to selectively 15N-labelled proteins, greatly facilitating the assignment of 15N-HSQC spectra. While the best yields are usually obtained with buffers containing high concentrations of potassium L-glutamate, preparation of selectively 15N-Glu labelled samples requires non-standard conditions. Among many compounds tested to replace the L-Glu buffer, potassium N-acetyl-L-glutamate and potassium glutarate were found to perform best, delivering high yields for all proteins tested, with preserved selectivity of 15N-Glu labelling. Assessment of amino-transferase activity by combinatorial 15N-labelling revealed that glutarate and N-acetyl-L-glutamate suppress the transfer of the 15N-alpha-amino groups between amino acids less well than …
Eoarchaean Crustal Growth In West Greenland (Itsaq Gneiss Complex) And In Northeastern China (Anshan Area): Review And Synthesis, Allen P. Nutman, Vickie C. Bennett, Clark R L Friend, Frances Jenner, Yusheng Wan, Dunyi Liu
Eoarchaean Crustal Growth In West Greenland (Itsaq Gneiss Complex) And In Northeastern China (Anshan Area): Review And Synthesis, Allen P. Nutman, Vickie C. Bennett, Clark R L Friend, Frances Jenner, Yusheng Wan, Dunyi Liu
Faculty of Science - Papers (Archive)
Eoarchaean crust in West Greenland (the Itsaq Gneiss Complex, 3870-3600 Ma) is >80% by volume orthogneisses derived from plutonic tonalite-trondhjemite-granodiorite (TTG) suites,3850, c. 3810 and c. 3710 Mahave some compositional similarities to modern island arc basalts (IAB), suggesting their origin by hydrous fluxing of a suprasubduction-zone upper mantle wedge. Most of the Eoarchaean tonalites match in composition high-silica, low-magnesian adakites, whose petrogenesis is dominated by partial melting of garnetiferous mafic rocks at high pressure. However, associated with the tonalites are volumetrically minor more magnesian quartz diorites, whose genesis probably involved melting of depleted mantle to which some slab-derived component had …
Synthesis And Ph-Responsive Properties Of Pseudo-Peptide Containing Hydrophobic Amino Acid Grafts, Rongjun Chen, Mark E. Eccleston, Zhilian Yue, Nighel K. H Slater
Synthesis And Ph-Responsive Properties Of Pseudo-Peptide Containing Hydrophobic Amino Acid Grafts, Rongjun Chen, Mark E. Eccleston, Zhilian Yue, Nighel K. H Slater
Faculty of Science - Papers (Archive)
Pseudo-peptidic polymers have been synthesised by grafting l-valine (PV), l-leucine (PL) and l-phenylalanine (PP) onto the pendant carboxylic acid moieties of a pH-responsive polyamide, poly(l-lysine isophthalamide). The pH-responsive aqueous solution properties of PV-75, PL-75 and PP-75 with a stoichiometric degree of substitution of 75 mol% have been compared with those of the parent poly(l-lysine isophthalamide) using UV-visible and fluorescence spectroscopy. At low concentrations (≤0.1 mg mL-1), the grafted polymers displayed pH-dependent conformation. The pH at the onset of hydrophobic association (pHh) and the pH range over which association occurred varied significantly between the different amino acid grafts. The pHh values …
Aryl Ethers From Arenediazonium Tetrafluoroborate Salts: From Neat Reactions To Solvent-Mediated Effects, James A. Shriver, Daniel P. Flaherty, Cameron C. Herr
Aryl Ethers From Arenediazonium Tetrafluoroborate Salts: From Neat Reactions To Solvent-Mediated Effects, James A. Shriver, Daniel P. Flaherty, Cameron C. Herr
Journal of the Iowa Academy of Science: JIAS
A general procedure for the synthesis of various aryl ethers via the thermal decomposition of benzenediazonium tetrafluoroborate salts is described. Studies performed in neat alcohol at 60°C gave aryl ethers in yields ranging from 0-73%. Upon completion of a series of reactions, the effect of solvent was explored to expand the scope and relevance of this procedure. It was found that even solvents that are traditionally non-nucleophilic gave rise to products including bi-aryls and N-aryl acetamides. The utilization of an ionic liquid, l-butyl-4-methylpyridinium tetrafluoroborate, resulted in yields comparable to reactions performed in neat alcoholic solvents.