Physical Sciences and Mathematics Commons^™

Seasonal Forage Availability And Diet Of Reintroduced Elk In The Cumberland Mountains, Tennessee, Jason Lee Lupardus

Masters Theses

The Tennessee Wildlife Resources Agency (TWRA) reintroduced elk (Cervus elaphus manitobensis) into the Cumberland Mountains, Tennessee over a 3-year period beginning in December 2000. We radio-collared 160 elk and monitored them by aerial telemetry from February 2001 to June 2003. Locations (n = 1450) were used in a geographic information system (GIS) to develop a core herd home range (789-ha sampling area) to assess elk seasonal forage use and availability. We monitored diet and resource availability from November 2003 to October 2004 by vegetation sampling and microhistological analysis of feces. Tall fescue (Festuca arundinacea; 35.1%) dominated …

The Role Of Phosducin-Like Protein And The Cytosolic Chaperonin Cct In G Beta Gamma Dimer Assembly, Ting Hu

Theses and Dissertations

Phosducin-like protein (PhLP), a G protein beta gamma subunit dimer binder and G protein signaling regulator, was suggested to regulate the activity of cytosolic chaperonin CCT by their high affinity interaction. In the present study, the three-dimensional structure of PhLP:CCT complex has been solved by cryoelectron microscopy. PhLP was found to bind only one of the chaperonin rings with both N- and C-terminal domains. It spans the central folding cavity of CCT and interacts with two opposite sides of the top apical region, inducing the constraining of the entry of the folding cavity. These findings support a putative role of …

Palladium-Imidazolium Carbene Catalyzed Heck Coupling Reactions And Synthesis Of A Novel Class Of Fluoroanthracenylmethyl Ptc Catalysts, Jiuqing Zhang

Theses and Dissertations

Palladium catalyzed Heck coupling with aryl and alkenyl halides has become a powerful means of carbon-carbon bond formation. This standard synthetic method has been developed to a high level of utility using various catalysts, conditions and substrates. Yet significant drawbacks remain, including poor reactivity, the need for high temperatures and base, limited substrate generality, and selectivity. Mixed products often suffer from olefin migration following insertion. N-Heterocyclic carbenes (NHC) have proven to be electron-rich donors which provide higher stability and reactivity than phosphines. In a previous paper reported by our research group the imidazolium-palladium carbene has proven to be highly efficient …

Total Syntheses Of (+)-Geldanamycin, (-)-Ragaglitazar, And (+)-Kurasoin A And Phase-Transfer-Catalyzed Asymmetric Alkylation, Erik J. Hicken

Theses and Dissertations

Geldanamycin possesses various biological activities as seen in the NCI 60 cell line panel (13 nM avg., 70 nM SKBr-3 cells). The predominant mode of action providing these unique results arises from the ability of geldanamycin (GA) to bind to the chaperone heat shock protein 90 (Hsp90). Despite its complicated functionality, the first total synthesis of GA was accomplished, which included two new reactions developed specifically to address the stereochemical features. The final step in the synthesis of GA was a demethylation-oxidation sequence to generate the desired para-quinone. This step could only be accomplished with HNO3/AcOH, producing GA in …

Specific Heat And Thermodynamic Properties Of Metallic Systems: Instrumentation And Analysis, Brian E. Lang

Theses and Dissertations

A small-scale adiabatic calorimeter has been constructed as part of a larger project to study nano-particles and to facilitate specific heat measurements on samples where it is difficult to obtain enough material to run on the current large-scale adiabatic apparatus. This calorimeter is designed to measure sample sizes of less than 0.8 cc over a temperature range from 13 K to 350 K. Specific heat results on copper, sapphire, and benzoic acid show the accuracy of the measurements to be better than ±0.4% for temperatures higher than 50 K. The reproducibility of these measurements is generally better than ±0.25%. Experimental …

Microcapsule Biosensors Based On Competitive Binding And Fluorescence Resonance Energy Transfer Assays, Swetha Chinnayelka

Doctoral Dissertations

Fluorescent sensing systems offer the potential for minimally invasive monitoring with implantable devices, but they require carrier technologies that provide suitable immobilization, accessibility, and biocompatibility while maintaining adequate response characteristics. Towards the development of this goal, a general design of a biosensor with the capability of detecting different metabolites was investigated. The approach is based on the encapsulation of a competitive binding assay in microcapsules and monitoring the changes in fluorescence resonance energy transfer (FRET) in the presence of analyte. To experimentally demonstrate this type of sensing system, glucose was chosen as the model target analyte. The design, fabrication, and …

Natural And Synthetic Viniferins Associated With The Grapevine Disease Young Vine Decline, David Michael Mcginnis

Chemistry & Biochemistry Theses & Dissertations

Grapevine disease has been thc subject of intense research amongst viticulturists over the last few decades, especially during the 1990's. There has been discoveries that suggest grapevine disease is commonly caused by fungal pathogens. One of the most common fungi that the vine may become infected by is known as Botrytis cinerea. B. cinerea is capable of attacking the grapevine which in turn will lead to bunch rot in the grape clusters. This disease has been researched in great detail during the past several years and is one of only few microorganisms that have actually been identified. Phaeoacremrmium chnlmydospnrum …

Polymer Microchips For Capillary Electrophoresis And Electric Field Gradient Focusing Of Biomolecules, Ryan Thomas Kelly

Theses and Dissertations

Polymeric materials have seen increasing use as microfluidic device substrates due to their low cost and the simplicity of templated fabrication procedures. I showed that poly(methyl methacrylate) (PMMA) microdevices could be enclosed in a boiling water bath, which allowed the seal to form more quickly than in conventional approaches, and enabled microchannels to remain hydrated throughout the bonding process. Microchip capillary electrophoresis (µ-CE) devices were fabricated using water-based enclosure, and a mixture of fluorescently labeled amino acids was separated in 30 s in these microchips. To create more robust capillary electrophoresis (CE) microdevices with improved separation performance, phase-changing sacrificial materials …

An Acyl Radical Cascade Model For The Total Synthesis Of Lyconadin A, Seth W. Grant

Theses and Dissertations

Lyconadin A (1) is a structurally unique Lycopodium alkaloid with antitumor properties, isolated from the club moss Lycopodium complanatum. We are developing a synthetic route to 1 based on a novel 7-exo-trig/6-exo-trig acyl radical cascade cyclization. The synthesis of model acyl radical cascade precursor 23 will be presented. Key features of this synthesis include the suppression of competing elimination during the alkylation of a hindered phenethyl bromide and the use of a lactone as a precursor to a compound bearing two differentially protected primary alcohols. An account of our studies on the model acyl radical cascade cyclization (23 to 24 …

Structure Elucidation Of Bioactive Compounds Isolated From Endophytes Of Alstonia Scholaris And Acmena Graveolens, Nicholas James Hundley

Theses and Dissertations

Alstonia scholaris is an evergreen tree native to Southeast Asia and Australia. It is commonly used as a medicinal plant throughout these regions. In the present study, an endophyte of the genus Xylaria was isolated from a stem of Alstonia scholaris, its mycelia and exudate extracted, and the extract assayed for growth inhibition of HeLa cancer cells in vitro. Several known compounds were isolated and identified based on NMR, infrared, and mass spectral data. The compounds identified are 19,20-epoxycytochalasin C; 19,20epoxycytochalasin D; and xylobovide. Two other compounds, fusaric acid and dehydrofusaric acid, were discovered in an endophyte of the Hypocreales …

Effect Of Melatonin And Dopamine In Site Specific Phosphorylation Of Phosducin In Intact Retina, Arinzechukwu Okere Nkemdirim

Theses and Dissertations

Phosducin (Pdc) is a 28 kDa binding partner for the G protein beta gamma subunit dimer (G-beta-gamma) found abundantly in the photoreceptor cells of the retina and pineal gland. In the retina, light-dependent changes in cAMP and Ca2+ control the phosphorylation of Pdc at serine 73 and 54, respectively, which in turn controls the binding of Pdc to G protein beta gamma subunit dimer . G protein beta gamma subunit dimer binding has been proposed to facilitate light-driven transport of G protein beta gamma subunit dimer from the site of phototransduction in the outer segment of the photoreceptor cell to …

Implications Of Spatial Autocorrelation And Dispersal For The Modeling Of Species Distributions, Volker Bahn

Electronic Theses and Dissertations

Modeling the geographical distributions of wildlife species is important for ecology and conservation biology. Spatial autocorrelation in species distributions poses a problem for distribution modeling because it invalidates the assumption of independence among sample locations. I explored the prevalence and causes of spatial autocorrelation in data from the Breeding Bird Survey, covering the conterminous United States, using Regression Trees, Conditional Autoregressive Regressions (CAR), and the partitioning of variance. I also constructed a simulation model to investigate dispersal as a process contributing to spatial autocorrelation, and attempted to verify the connection between dispersal and spatial autocorrelation in species' distributions in empirical …

Fluorescent Photoinduced Electron Transfer (Pet) Cation Sensors Derived From Natural Products, Kenny Loo

Theses, Dissertations and Culminating Projects

Two new second generation fluorescent switches that are based on the chromophore-spacer-receptor architecture are reported in this thesis. The fluorescence modulation of these switches is a result of quenching or initiating photoinduced electron transfer (PET) between the chromophore and the receptors due to cation binding. These two molecules utilize an alkaloid, anabasine, as the proton receptor, and function as fluorescent off-on-off switches with decreasing pH. One of these molecules also utilizes a coumarin as the chromophore, making it the first rationally designed fluorescent PET system based on natural products. Further studies of two well-known first generation fluorescent switches to achieve …

Angiotensin Ii-Mediated Regulation Of The Human Angiotensin Ii Type 1 Receptor Gene, Xylophone Vijai Aasee Victor

Theses and Dissertations

The physiological responses of angiotensin II (Ang II) are mediated across the cell membrane through the angiotensin II type 1 receptor (AT1R), a heptahelical membrane protein coupled to trimeric G-proteins on the cytosolic side. AT1R on binding its ligand, Ang II, leads to downregulation of cell-surface receptor and also its mRNA. We have investigated whether the 3'- and 5'-untranslated regions of the human AT1R mRNA mediate the degradation of hAT1R mRNA by post-transcriptional mechanisms in human adrenocortical carcinoma cell line (H295R cells). Protein kinase C (PKC) activator, phorbol-12-myristate-13-acetate (PMA), showed that the downregulation of hAT1R mRNA is mediated by the …

Development Of A Model For The Kinetics And Mechanism Of Nitrogenase, Phillip E. Wilson

Theses and Dissertations

Nitrogenase has a central role in the global nitrogen cycle as the enzyme that catalyzes the reduction of atmospheric N2 to NH3. Fixed nitrogen is generally limiting in the environment and in agriculture, so nitrogenase has received much attention as an alternative to nitrogen fertilizers. Characterizing the mechanism of nitrogenase is the goal of this work. The molybdenum nitrogenase enzyme system is comprised of the MoFe protein and the Fe protein. Interactions between these proteins and nucleotides are crucial to catalysis. An important approach to characterize these interactions is to correlate the kinetics of nitrogenase catalysis to a mechanism based …

Interannual Variation Of Stratification In Lower Chesapeake Bay, Christopher S. Katzenmiller

OES Theses and Dissertations

Stratification in the water column can prove to be an important indicator to the state of the water column and ecosystem. The focus of this research is to evaluate trends in stratification in the Lower Chesapeake Bay. Detailed analysis was performed on a 14 year data set to study interannual variation in the region of study. Potential energy anomaly was used to quantify stratification. Potential energy anomaly is the amount of energy required to mix a water column. It is determined from the vertical density structure of density. Potential energy anomaly is the departure of potential energy from climate conditions. …

Analyzing The Effects Of Various Sign Types Used By The Bureau Of Land Management For Desert Cleanup And Restoration Projects, Christopher Linehan

UNLV Theses, Dissertations, Professional Papers, and Capstones

What is the effectiveness of attempts to curb illegal dumping with prompts on signs posted in the area? Do positive friendly language prompts such as “Restoration in Progress Please Do Not Disturb” generate more positive attitudes and intentions towards illegal dumping problems, than less friendly prompts such as “No Dumping Allowed”?

Results, although inconclusive, did however show a slight trend towards the negative language signs.

Total Suspended Solids In Las Vegas Wash, Mika Koponen

UNLV Theses, Dissertations, Professional Papers, and Capstones

Erosion along the Las Vegas Wash (Wash) has increased steadily along with the rapidly increasing population. The focus of this study is to find out if there is a relationship between lower total suspended solids rate (TSS) and the construction of erosion control structures along the Wash. The study was conducted between 7/15/2003 and 11/29/2004.

The method was water sampling on 7 sites and the samples were analyzed at Southern Nevada Water Systems (SNWS) laboratory at Saddle Island in Boulder City. Additional water quality data was also collected using Quanta, a device measuring different parameters in water. In this study …

Using Domination To Analyze Rna Structures., Travis Reves Coake

Electronic Theses and Dissertations

Understanding RNA molecules is important to genomics research. Recently researchers at the Courant Institute of Mathematical Sciences used graph theory to model RNA molecules and provided a database of trees representing possible secondary RNA structures. In this thesis we use domination parameters to predict which trees are more likely to exist in nature as RNA structures. This approach appears to have promise in graph theory applications in genomics research.

A Comparison Of Population Sizes And Number Of Captures For The Desert Woodrat (Neotoma Lepida) In Two Microhabitats In The Las Vegas Wash, Nevada, Jayson Barangan

UNLV Theses, Dissertations, Professional Papers, and Capstones

Planned removal and eradication of saltcedar in the Las Vegas Wash, Nevada could potentially generate adverse impacts on present desert woodrat (Neotoma lepida) populations inhabiting the area. Consequently, research was conducted investigating population sizes of N. lepida in two distinct microhabitat types, saltcedar (Tamarix sp.) and mesquite/quailbush (Prosopis sp./Atriplex lentiformis). The results of this study will aid in gauging the effects of the changes in vegetation once restoration work is completed and assist with logistical scheduling for implementation of control measures. Mark-recapture field techniques were utilized for data collection from July 2002 to June 2003. Population estimates were calculated using …

Observed Omnivory In The Herbivorous Blue Tilapia (Oreochromis Aureus), Stephen M. Oliveira Jr.

UNLV Theses, Dissertations, Professional Papers, and Capstones

Gila seminuda, a native endangered species, and Oreochromis aureus, a nonnative invasive species, share the same habitat in the form of the Reid Gardner Power Plant intake ponds. The purpose of this study is to characterize the feeding habits of Oreochromis aureus within the Reid Gardner Power Plant intake ponds and address the potential predation habits of Oreochromis aureus. Stomach contents of O. aureus were identified and analyzed using chi-square and log-likelihood ratio techniques, with respect to mass, length, girth, gender, or spatial distribution of samples. Results showed omnivorous behavior with a 7.00% predatory/prey relationship, and no difference of feeding …

Alterations In Uterine And Placental Sodium Pump Abundance May Contribute To The Onset Of Mouse Labor, Carlos Jacob Vance

Theses and Dissertations

Objective: Reductions in sodium pump (SP) abundance can give rise to increases in contractile force in uterine and vascular smooth muscle as well as an increased secretion in secretory cells, including potentially those of the placenta. To determine whether the mouse might serve as a model for human pregnancy in terms of the SP and to determine whether changes in SP abundance anticipate or follow labor, we studied pregnant mice over the final trimester of their pregnancy.

Study Design: C57Bl6 dams (n=46) were bred and studied during their pregnancy. Animals (n=4) were sacrificed at specific gestational time points. Other mice …

Ion Transmission In The First Vacuum Stage Of An Inductively Coupled Plasma Mass Spectrometer, Jeffrey Harris Macedone

Theses and Dissertations

The inductively coupled plasma mass spectrometer (ICP-MS) is the instrument of choice for trace and ultra-trace elemental analysis. However, the ICP-MS suffers from matrix effects. Matrix effects occur when instrument response varies as the composition of the sample matrix is changed. Matrix effects, or non-spectroscopic interferences, limit the accuracy of routine analysis. Identification of the sources of matrix effects provide a basis for reducing or eliminating them. As inaccuracies in the ICP-MS are more severe than those in the ICP atomic emission spectrometer, the problem may be due, at least in part, to the vacuum interface used to couple the …

Grassland And Woodland Bird Occurrence And Habitat Selection In The Prairie-Forest Transition Zone Of Minnesota, Thomas Robert Cooper

Electronic Theses and Dissertations

Numerous species of woodland and grassland nesting birds have experienced population declines in the midwestern United States. Habitat loss and habitat fragmentation are suspected to be two of the underlying causes for the population declines of woodland and grassland birds. The Minnesota Valley Wetland Management District (WMD) covers 14 counties in southeastern Minnesota in the transition zone between forested and prairie ecoregions. Little information is available relative to which birds are using grassland or woodland habitat within the WMD and how their presence or absence is related to the available habitat conditions. Therefore, my study objectives were to: 1) determine …

Effects Of Human Disturbance On The Breeding Success Of Eastern Bluebirds (Sialia Sialis), Caitlin Rebecca Kight

Dissertations, Theses, and Masters Projects

No abstract provided.

Eye Pathologies Found In Several Decapod Crustaceans, Andrea M. Maniscalco

Dissertations, Theses, and Masters Projects

No abstract provided.

Application Of Survival Analysis Methods To Pulsed Exposures: Exposure Duration, Latent Mortality, Recovery Time, And The Underlying Theory Of Survival Distribution Models, Yuan Zhao

Dissertations, Theses, and Masters Projects

Ecotoxicologists adopted median lethal concentration (LC50) methods from mammalian toxicology. This conventional LC50 approach has shortcomings. Fixing the exposure duration and selecting the 50% mortality level result in loss of ecologically relevant information generated at all other times. It also ignores latent mortality that can manifest after exposure ends. as a result, it cannot adequately predict pulsed exposure effects in which concentration, duration, and frequency of pulses change through time. The underlying theory of the dose-response models used to calculate LC50 values, stochastic versus individual effective dose (IED) theory, has not been tested rigorously either. In this study, the effects …

Synthesis Of Novel Porphyrazines And Binding Studies To Dna Quadruplexes, Carlos Ramirez

Seton Hall University Dissertations and Theses (ETDs)

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Reproductive Attributes Of Polynoid Polychaetes From Hydrothermal Vents On The East Pacific Rise, Jessica Lynn Wallace

Dissertations, Theses, and Masters Projects

No abstract provided.

Digital Vertical Aerial Camera System For High-Resolution Site Inspections In Conservation Easment Monitoring, Kenton Williams

Electronic Theses and Dissertations

A three-level satellite to ground monitoring scheme for conservation easement monitoring has been implemented in which high-resolution imagery serves as an intermediate step for inspecting high priority sites. A digital vertical aerial camera system was developed to fulfill the need for an economical source of imagery for this intermediate step. A method for attaching the camera system to small aircraft was designed, and the camera system was calibrated and tested. To ensure that the images obtained were of suitable quality for use in Level 2 inspections, rectified imagery was required to provide positional accuracy of 5 meters or less to …