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Physical Sciences and Mathematics Commons

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Full-Text Articles in Physical Sciences and Mathematics

Evaluation Of Electrophoretic Protein Extraction And Database-Driven Protein Identification From Marine Sediments, Eli K. Moore, Brook L. Nunn, Jessica F. Faux, David R. Goodlett, H. Rodger Harvey May 2012

Evaluation Of Electrophoretic Protein Extraction And Database-Driven Protein Identification From Marine Sediments, Eli K. Moore, Brook L. Nunn, Jessica F. Faux, David R. Goodlett, H. Rodger Harvey

OES Faculty Publications

Intact proteins comprise a major component of organic carbon and nitrogen produced globally and are likely an important fraction of organic matter in sediments and soils. Extracting the protein component from sediments and soils for mass spectral characterization and identification represents a substantial challenge given the range of products and functionalities present in the complex matrix. Multiple forms of gel electrophoresis were evaluated as a means of enhancing recovery of sedimentary protein before proteomic characterization and compared with a direct enzymatic digestion of proteins in sediments. Resulting tryptic peptides were analyzed using shotgun proteomics and tandem mass spectra were evaluated …


Guest Editors' Introduction, Jianer Chen, Ion Măndoiu, Raj Sunderraman, Jianxin Wang, Alexander Zelikovskiy Jan 2012

Guest Editors' Introduction, Jianer Chen, Ion Măndoiu, Raj Sunderraman, Jianxin Wang, Alexander Zelikovskiy

Computer Science Faculty Publications

This Supplement includes a selection of papers presented at the 7th International Symposium on Bioinformatics Research and Application (ISBRA), which was held on May 27-29, 2011 at Central South University in Changsha, China. The technical program of the symposium included 36 extended abstracts presented orally and published in volume 6674 of Springer Verlag’s Lecture Notes in Bioinformatics series. Additionally, the program included 38 short abstracts presented either orally or as posters. Authors of both extended and short abstracts presented at the symposium were invited to submit full versions of their work to this Supplement. Following a rigorous review process, 19 …