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Full-Text Articles in Physical Sciences and Mathematics
Rev1 Protein Purification, Martha Stein, Karissa Carlson, Emily Grace
Rev1 Protein Purification, Martha Stein, Karissa Carlson, Emily Grace
Celebration of Research
1 in every 15-30 million nucleotides can be considered a mutation in healthy individuals. Replication of damaged DNA leads to genetic mutations. Genetic mutations lead to disorders and cancer. Rev1, a Y family polymerase, prevents minor mutations, such as abasic sites and exocyclic guanines, in the template strand from shutting down the process of replication. Our mutant Rev1, R324G/L325G, has replaced both the arginine located on nucleotide base number 324 and the leucine on nucleotide base number 325 with glycine. The arginine and leucine have more complex side chains groups with specific hydrophobic/hydrophilic properties. By replacing them with glycine, the …
Slide Preparation For Dna Attachment For Use In Optical Tweezers, Dylan J. Kirkeby, Karissa Carlson, Emily Grace, Eric Jones
Slide Preparation For Dna Attachment For Use In Optical Tweezers, Dylan J. Kirkeby, Karissa Carlson, Emily Grace, Eric Jones
Celebration of Research
Our research team, ISLAND CURE, is a multidisciplinary team of professors and undergraduate students with the goal to design and build instruments to make biological measurements on a limited budget. One of the apparatuses we are designing, is optical tweezers, which are a Nobel Prize-winning technology capable of trapping microscopic and sub-microscopic particles using a laser beam. Using a 1064 nm beam, we will trap a single strand of DNA using beads and this will enable us to exert minute forces upon the DNA. This experiment will give us a better understanding of the forces on damaged DNA; specifically, the …
Utilization Of Escherichia Coli For The Growth Of Y Family Dna Polymerase Rev1 And Gstrap Column For Purification, Alexa Olguin, Alaena Trevino, Martha Stein, Karissa Carlson, Emily Grace
Utilization Of Escherichia Coli For The Growth Of Y Family Dna Polymerase Rev1 And Gstrap Column For Purification, Alexa Olguin, Alaena Trevino, Martha Stein, Karissa Carlson, Emily Grace
Celebration of Research
Rev1 is a Y family DNA polymerase that specializes in translesion DNA synthesis. Rev1 is unique in that it preferentially incorporates dCTP in the growing DNA strand, regardless of the templating base. This is because the template base is evicted from the active site and a template amino acid, arginine 324 (R324) acts as the template for the incoming dCTP. We hypothesize that arginine 324 and the neighboring leucine (L325) facilitate the eviction of the DNA template from the active site. To test this hypothesis, we worked to purify R324G/L325G Rev1 double mutant for the purpose of X-ray crystallographic examination …