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Investigation Of Membrane Receptors’ Oligomers Using Fluorescence Resonance Energy Transfer And Multiphoton Microscopy In Living Cells, Ashish K. Mishra
Investigation Of Membrane Receptors’ Oligomers Using Fluorescence Resonance Energy Transfer And Multiphoton Microscopy In Living Cells, Ashish K. Mishra
Theses and Dissertations
Investigating quaternary structure (oligomerization) of macromolecules (such as proteins and nucleic acids) in living systems (in vivo) has been a great challenge in biophysics, due to molecular diffusion, fluctuations in several biochemical parameters such as pH, quenching of fluorescence by oxygen (when fluorescence methods are used), etc.
We studied oligomerization of membrane receptors in living cells by means of Fluorescence (Förster) Resonance Energy Transfer (FRET) using fluorescent markers and two photon excitation fluorescence micro-spectroscopy. Using suitable FRET models, we determined the stoichiometry and quaternary structure of various macromolecular complexes. The proteins of interest for this work are : (1) sigma-1 …