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Full-Text Articles in Physical Sciences and Mathematics
Analysis Of Phytoestrogens By High Performance Liquid Chromatography, Anne A. Grippo, Yan Xie, Benjamin L. Rougeau, William V. Wyatt
Analysis Of Phytoestrogens By High Performance Liquid Chromatography, Anne A. Grippo, Yan Xie, Benjamin L. Rougeau, William V. Wyatt
Journal of the Arkansas Academy of Science
Phytoestrogens are biochemicals synthesized in plants which mimic steroidal estrogen activity in mammals. Analysis of these compounds in the legumes which produce them and in body fluids is important to the study of their physiological effects. High pressure liquid chromatography (HPLC) has been found to be an efficient and sensitive method of identification and quantitation of isoflavonoids, one class of phytoestrogen. Here we report the separation of three isoflavonoids, biochanin A, genistein and daidzein using an HPLC system with a Cg reverse phase column and a linear gradient mobile phase containing acetonitrile and acetic acid/water (10/90, v/v) over 60 minutes. …
Effects Of Predation On Two Species Of Stream-Dwelling Crayfish (Orconectes Marchandi And Cambarus Hubbsi) In Pool And Riffle Microhabitats, Heidi Dukat, Daniel D. Magoulick
Effects Of Predation On Two Species Of Stream-Dwelling Crayfish (Orconectes Marchandi And Cambarus Hubbsi) In Pool And Riffle Microhabitats, Heidi Dukat, Daniel D. Magoulick
Journal of the Arkansas Academy of Science
Community structure may be governed by many abiotic and biotic factors. Of the biotic factors, predation is often considered to be critical in structuring freshwater stream communities. In the Warm Fork of the Spring River, the crayfish Cambarus hubbsi is found mainly in riffles, whereas the crayfish Orconectes marchandi is found in high numbers in pools. We hypothesized that predation, mainly by fish, is a factor causing this segregation. Higher predation rates for C. hubbsi than 0. marchandi in the pools and higher predation rates for 0. marchandi than C. hubbsi in the riffles were expected. A transplant tethering experiment …