Physical Sciences and Mathematics Commons^™

Wilderness And The Geotag: Exploring The Claim That "Geotagging Ruins Nature" In The Alpine Lakes Wilderness, Wa, Mara Gans

All Master's Theses

This research explores the claim that “geotagging ruins nature” by quantifying and qualifying patterns in geotag use and visitors’ experiences in the Alpine Lakes Wilderness, in Washington, United States. Many have raised concerns that geotags increase recreational visitation to public lands, which subsequently contributes to negative resource impacts. Others, however, claim that geotagging has made the outdoors more accessible to less privileged communities and raise concerns that condemning geotags will perpetuate the exclusion of certain groups from outdoor recreation. This debate is studied within federally designated Wilderness, which is legally defined as “untrammeled by man,” a definition rooted in problematic …

An Ecological Comparison Between Resource Subsidies: Pacific Lamprey (Entosphenus Tridentatus) And Pacific Salmon (Oncorhynchus Spp.), Jocelyn Wensloff

All Master's Theses

Historically, oligotrophic Pacific Northwest (PNW) streams received annual returns of spawning anadromous fish that provided resource subsidies in the form of marine-derived nutrients (MDN), thus driving stream food web productivity. To date, many studies in the PNW have focused on Pacific salmon (Oncorhynchus spp.) as a resource subsidy, overlooking other anadromous fish species such as Pacific lamprey (Entosphenus tridentatus). Both Pacific salmon and Pacific lamprey are culturally important to PNW tribes for ceremonial, medicinal, and subsistence purposes, and have been since time immemorial. Unfortunately, both salmon and lamprey populations are in decline. Historically, lamprey have been disregarded …

Development Of Environmental Metabarcoding Analysis For Use In Ecological Studies Of Aquatic Fungal And Oomycete Communities Using Nanopore Sequencing, Douglas Bennett

All Master's Theses

One of several factors contributing to amphibian decline are organisms called water molds (oomycetes), which parasitize and kill amphibian and fish eggs, larvae, and occasionally adults. Amphibian decline is one of the most difficult issues facing ecologists and conservationists to date; half of amphibian species are in severe decline and one third of amphibian species are facing extinction, threatening the stability of ecosystems globally. Multiple oomycete species are known to cause the disease saprolegniasis and contribute to amphibian decline; however, the full range of species involved is not presently known. This study evaluated the Oxford Nanopore minION DNA sequencer (R9.2 …

Using Cuda To Enhance Data Processing Of Variant Call Format Files For Statistical Genetic Analysis, Heather Mckinnon

All Graduate Projects

Utilizing the power of GPU parallel processing with CUDA can speed up the processing of Variant Call Format (VCF) files and statistical analysis of genomic data. A software package designed toward this purpose would be beneficial to genetic researchers by saving them time which they could spend on other aspects of their research. A data set containing genetics from a study of trichome production in Mimulus guttatus, or yellow monkey flower, was used to develop a package to test the effectiveness of GPU parallel processing versus serial executions. After a serial version of the code was generated and benchmarked, OpenACC …

Evaluation Of The Relationship Between Land Use And Water Quality In Kittitas County, Wa, Lindsay Schulz

All Master's Theses

Water in Kittitas County is extremely valuable since it supports farming, recreation, and cultural activities, as well as environmental processes and a diversity of biological life while providing many ecosystem services. However, land conversions required by agricultural and urban land uses can negatively impact water quality and the biological function of the stream. I studied how forested, agricultural, and urban land use affect six streams. Fourteen sites were sampled, once each in July, August, and September 2019. Land use was calculated as a percentage of forested, agricultural, and urban land use within a 100-m buffer of the stream, upstream of …

An Analytical Examination On The Effects Of Vegetarian And Omnivorous Diets On C-Reactive Protein, Aletha Kleis

Undergraduate Honors Theses

There is a lack of research regarding how following a vegetarian or omnivores diet effects C-Reactive Protein (CRP) levels of people as seen through results from an analysis of data gathered from the National Health and Nutrition Examination Survey (NHANES). The level of CRP is a reflection of how much inflammation there is in one’s body and is a popular indicator of risk for heart disease. Thus, in this research, I use the NHANES data to look at the relationship of CRP levels of people who identified themselves as vegetarian or not, while also considering the general healthiness of each …

Sampling The Local Fare: Fishes At The Sam Israel House Pit (45gr76), Soap Lake, Washington, Adam Fruge

All Master's Theses

The Sam Israel site is a precontact archaeological complex with numerous fish bones at the north end of Soap Lake, Washington. Excavated in 1976, the fish remains recovered from there were never fully analyzed prior to this research. Since this inland Columbia Plateau site had thousands of fish bones, it contained untapped potential for our understanding of ancient local fish procurement. As such, I conducted a detailed analysis of 2,862 fish bone specimens from the Sam Israel House Pit locus to: study a larger sample of fish bones in greater detail than was done before; compare the distribution of fishes …

Distribution Of Invasive Plant Species On Debris Cones At Mount Rainier National Park, Shaun Morrison

All Master's Theses

Invasive plant populations within Mount Rainier National Park are a biological and ecological threat to the unique mountain landscape. A better understanding of their distribution and transport within the landscape is needed to improve invasive species monitoring for National Park Service management. This study investigates how invasive plant populations in Stevens Canyon are utilizing the debris cone disturbances and associated geomorphic processes to facilitate movement within the park. Vegetation transects were performed along Stevens Canyon Road (to observe the roadside community composition) and on the debris cone features (to observe species movement from the roadside). These vegetation observations are presented …

Holocene Fire History Reconstruction Of A Mid-Evaluation Mixed-Conifer Forest In The Eastern Cascades, Washington, Zoe Rushton

All Master's Theses

Fire histories of mid-elevation mixed-conifer forests (MEMC) are uncommon, particularly in the eastern Cascades of Washington. As a result, fire regimes and the effects of 20th century fire suppression in these forests are not well understood. In the summer of 2014 a 7.80 meter-long sediment core was extracted from Long Lake, located approximately 45 km west of Yakima, WA, which exists in a grand fir-dominated mixed-conifer forest. Fire activity for the Long Lake watershed was reconstructed using macroscopic charcoal analysis and pollen analysis was used to reconstruct vegetation change through time. Charcoal results show low fire activity in the early …

Measuring Trace Element Concentrations In Artiodactyl Cannonbones Using Portable X-Ray Fluorescence, Joshua L. Henderson

All Master's Theses

Artiodactyl bones are the most common faunal remains found in Washington prehistoric archaeology sites, but they are often too fragmented to accurately identify a family, genus, or species. Traditional faunal analysis can only organize unidentifiable bone fragments into size class, and chemical methods often require the destruction of bone samples. In this thesis research, I tested a new, nondestructive faunal analysis technique using portable X-ray fluorescence (pXRF) to measure trace element concentrations in comparative collection and archaeological bone samples. Using cannonbones from five different artiodactyl species, I collected trace element data from 50 comparative collection specimens and 18 archaeological specimens …

A Novel Snp Genotyping Technique To Determine Orangutan Relatedness And Genetic Diversity At Camp Leakey In Tanjung Puting, Central Kalimantan, Ruth Ella Linsky

All Master's Theses

Genetic studies of dispersal patterns in wild populations of orangutans (Pongo spp.) have sought to confirm behavioral observations that female orangutans tend to stay near their natal range while males disperse. In order to genotype a previously unsampled wild population of endangered orangutans at Tanjung Puting National Park I developed novel application of a methyl based magnetic capture for enrichment of fecal DNA and commercial human targeted single nucleotide polymorphism (SNP) microarray technology. I confirmed results of this new genotyping technique through standard microsatellite short tandem repeat (STR) micro-capillary genotyping. I estimate genetic diversity and relatedness (r) …

Intertidal Habitat Utilization By Endangered Green Sturgeon (Acipenser Medirostris), Willapa Bay, Washington, Luke Stilwater

All Master's Theses

This study looks at a portion of the designated critical habitat for the threatened southern distinct population segment of green sturgeon (Acipenser medirostris) in Willapa Bay, Washington. Willapa Bay is an intermediate size (258.7mi2) estuary on the southwest coast of Washington State, approximately 30 miles north from the mouth of the Columbia River. Recent studies completed by the National Marine Fisheries Service have shown that significant aggregations of green sturgeon in Willapa Bay occur during the late summer months, and foraging activity for burrowing ghost shrimp (Neotrypaea californiensis) is evidenced by small round feeding pits (30-60 cm diameter) in the …

Two Post-Glacial Sagebrush Steppe Fire Records At The Wildland-Urban Interface, Eastern Cascades, Washington, Dusty Pilkington

All Master's Theses

Recent increases in large fires in the rapidly developing wildland-urban interface (WUI) areas of central Washington, where development intermixes with wildland fuels, contribute to federal firefighting costs exceeding of $1 billion annually. In addition, cheatgrass (Bromus tectorum) invasion and anthropogenic-caused warming shorten fire return intervals while lengthening fire seasons. These climatic, ecological, economic, and social factors combine with fuel accumulation resulting from historic fire suppression to threaten lives and property in the WUI. To plan for safe growth in WUI areas, long-term fire histories are needed to expand understanding of past fire regimes in an understudied ecosystem, sagebrush …

Modeling Sex-Specific Seasonal Habitat Selection For Elk (Cervus Elaphus) In Central Washington, Lewis Meyers

All Master's Theses

Elk (Cervus elaphus) are generalist herbivores, exploiting a variety of environments. I studied habitat selection and sexual segregation of the Colockum elk herd in central Washington. I used a resource selection probability function (RSPF) to evaluate habitat use by males and females during summer and winter seasons. I assisted Washington State Department of Fish and Wildlife (WDFW) in capturing and placing GPS collars on males, and used an existing GPS dataset from females to investigate the extent to which sexual segregation was occurring in the Colockum herd. During summer, males selected steep slopes on north, west and south …

Do Elevated Levels Of Glutathione Decrease The Toxicity Of Plasticizers In Mouse Liver Cells?, Khatoon Albahrani

All Master's Theses

Phthalates are esters of phthalic acid that play a crucial role in the manufacturing industries for enhancing the properties of plastic materials. Di-(2-ethylhexyl) phthalate (DEHP) is one of the most common phthalates used in a variety of products including food packaging and medical equipment. Their use has caused public health concerns because of their toxic effects on reproductive and developmental processes. This study aims to investigate the response of two mouse hepatoma cell lines to the effects of DEHP and its bioactive metabolite mono (2-ethylhexyl) phthalate (MEHP). Cells were exposed to four concentrations (10, 50, 100, and 300 µM) of …

Defining Biodiversity: A Local Assessment Of The Tahuayo River, Peru Using Self-Directed Photography, Rozsika D. Steele

All Master's Theses

The Área de Conservación Regional Comunal Tamshiyacu Tahuayo (ACRCTT), located in Loreto, Peru, protects 420,000 hectares of the Amazon basin. In 2009, the ACRCTT received formal government recognition after three decades of advocacy and conservation work by resident communities. Local resource users who live a subsistence lifestyle possess sophisticated Traditional Ecological Knowledge (TEK) that can be used to identify which constituents of biodiversity are culturally relevant. This information can help resource managers develop an operational definition of biodiversity. Self-directed photography is a research method that allows participants the opportunity to direct data collection and empowers them to visually communicate their …

Resolving Gnetum Evolutionary History, Angela Mcfadden

All Master's Theses

Gnetum are non-flowering seed plants of the tropics, indigenous to South America, Africa, and Asia. This group of about 40 species is fascinating to botanists because it shares distinctive morphological characteristics with flowering plants, such as broad leaves, woody stems, and flower-like strobili. There are still questions surrounding the relationships within the genus of Gnetum. With that in mind, I focused my work on generating phylogenetic hypotheses, using two molecular data sets: a concatenation of over 60 different chloroplast genes (66,815 base pairs), and the whole chloroplast genome (128,772 base pairs). This allowed me to compare the two phylogenies …

Provisioning And Its Effects On The Social Interactions Of Tibetan Macaques (Macaca Thibetana) At Mt. Huangshan, China, Brianna I. Schnepel

All Master's Theses

The dispersal patterns of food resources has a significant effect on the composition of primate groups and social interactions within those groups. Humans often alter the dispersal of food. Non-humans often use affiliative behaviors to elicit tolerance or support from other group members. I investigated whether provisioned food resources alter the social interactions and group dynamics of Macaca thibetana. All-occurrence sampling and scan sampling were used for data recorded by camera traps. Trail-cameras were placed at six locations that contain natural and human food resources and recorded 60-second videos. Social behavior and proximity of the monkeys were recorded. I …

Quantifying Channel Responses To The Removal Of The Glines Canyon Dam In The Middle Reach Of The Elwha River, Washington, Bryon J. Free

All Master's Theses

Four different study sites throughout the middle reach of the Elwha River were monitored before, during, and after the dam removal process over a period of two years from 2012-2014. The complexity of the river geometry was a major factor in the ability of the river to trap and accumulate the new influx of woody debris and sediment from the dam removal, which influenced the response of the river channel. The change that occurred was quantified by using repeat Terrestrial LiDAR (TLS), sediment distribution surveys, and large woody debris mapping techniques. The morphologic changes that occurred during this time were …

Applying Wetland Rating Systems To Assess Functions Of Depressional Wetlands Created By A Mass Wasting Feature, Table Mountain, Washington, Thomas S. Wachholder

All Master's Theses

The formation of wetlands in the Swauk Watershed has been primarily controlled by mass wasting events, which includes landslide activity. Landslide activity has been the primary influential process in shaping the landscape where wetland systems have formed on the surface of landslide deposits. The wetland sites used in this study, near the base of Table Mountain, were chosen because they inhabit the same ancient landslide, have the same underlying geology, and vary in aspect and elevation. The elevational gradient of the sites ranges from 1300 – 1600 m and the individual wetlands differ in terms of north- and south-facing aspects. …

The Elwha River Restoration: Landscape Change, Salmon, And Sense Of Place, Kelseyanne Johnson

All Master's Theses

Removal of the Elwha River dams to restore the ecosystem and salmon fisheries is the largest project of its kind in the history of the United States. Spanning decades and affecting generations, this project has changed and will continue to change the landscape. This research examined the influence of the anticipated return of salmon on 18 key stakeholders' sense of place of the Elwha River. For almost all stakeholders, changes to the Elwha landscape are welcomed as they expect that salmon will restore the ecosystem to its natural state. Narrative data revealed dominant themes in participants' sense of place expressed …

Evaluating The Utility Of Beaver Reintroduction Programs For Enhancing Habitat For Rainbow Trout And Steelhead, Jonathan Rodger Hegna

All Master's Theses

Beaver reintroduction programs are increasingly being viewed as a way to enhance salmonid habitat and production. However, the actual effectiveness of using beavers as a habitat enhancement tool for ESA listed steelhead Oncorhynchus mykiss populations is unknown. We examined the type of habitat, at both the microhabitat and mesohabitat levels, preferred by steelhead in three small streams in the upper Yakima Basin, WA through standard snorkel surveys and habitat measurements. Our results suggest that steelhead in small streams strongly prefer (relative to availability) microhabitats that have deeper water(> 30 cm), slow stream velocities(< 0 .05 mis), and complex cover types. Habitat partitioning among the size-classes (small< 50 mm, medium 50-90 mm, large> 90 mm total length, TL) principally operated …

Experiments To Measure The Effects Of Timber Harvesting Equipment On Surface Lithic Scatters, Douglas J. Baughman

All Master's Theses

The importance of cultural resource preservation cannot be overstated; however local economies are at least as important. Due to conservative archaeological site protection practices in Region 5 of the United States Forest Service, the economy of Northeastern California is being adversely affected. In an attempt to help the Forest Service make more informed management decisions and improve the Northeastern California economy, I undertook experiments on the effects of timber harvesting on lithic scatters on Modoc National Forest. The experiments involved placement of 225 glass tiles (proxy lithics) in each of three plots subject to vehicle traffic and log dragging by …

An Assessment Of Potential Habitat Corridors And Landscape Ecology For Long-Tailed Macaques (Macaca Fascicularis) On Bali, Indonesia, Mark Warren Southern

All Master's Theses

The relationship between Balinese long-tailed macaques, Bali's cultural settings, and Bali's physical settings was studied. A Geographical Information System (GIS) database was developed and analyzed to determine if forest corridors exist on Bali that may provide habitat connectivity between 42 Balinese long-tailed macaque troops. The GIS database was also analyzed to determine if the landscape type that has the highest percentage of overlap with the 42 Balinese long-tailed macaque home range sites is forest. The results indicate that connectivity between troops located within Bali's western region is significantly higher than that for troops located in Bali's eastern region. The results …

A Spatial And Temporal Analysis Of Riparian Vegetation Along Satus Creek On The Yakama Indian Reservation, Kathryn Gellenbeck

All Master's Theses

Satus Creek provides critical habitat for the Yakima River Basin steelhead. A diverse community of riparian vegetation is important for healthy fish habitat; vegetation changes can affect shade, cover, channel structure, water quality, and food availability. The purpose of this thesis is to analyze and illustrate riparian vegetation change, both temporally and spatially, along three separate reaches of Satus Creek. A Geographic Information Systems approach was applied to assess the vegetation change by comparing plant species composition and density on 1949 and 1995 aerial photographs. The GIS approach allowed patterns and trends in the vegetation to be identified. In less …

Impacts Of Irrigation Development On Anadromous Fish In The Yakima River Basin, Washington, Robert L. Tuck

All Master's Theses

Prior to Euroamerican development, the Yakima River Basin was a major producer of salmon. Total runs of approximately 800,000 have declined to 3,000-5,000, or less than 1% of original run size. Three species are extinct in the basin, including summer chinook, coho, and sockeye.

Irrigation development, including the construction of unscreened diversions, the blockage of spawning and rearing habitat by reservoir dams, and the dewatering of spawning and rearing habitat, began in the mid-1800's and today totals approximately 500,000 acres. Historical records provide a wealth of information documenting irrigation development and its consequences on anadromous fish populations.

A Study Of The Summer Steelhead, Oncorhynchus Mykiss In Several Intermittent Tributaries Of The Satus Creek Basin, Washington, Joel Denney Hubble

All Master's Theses

The summer steelhead, Oncorhynchus mykiss population was studied in three intermittent tributaries in the Satus Creek Basin. There was a strong tendency for post emergent fry, resulting from redds located in intermittent reaches to move downstream. However, survival to perennial flow conditions was very low. Steelhead production in Dry Creek appears to be lower than in Logy Creek, because of elevated water temperatures and minimal spring and summer stream flow.

The Enzymatic Oxidation Of Aldehydes By Xanthine Oxidase, Duane Beal Gregory

All Master's Theses

As early as sixty years ago, the existence of an enzyme from mammalian tissues which catalyzed the oxidation of xanthine and hypoxanthine was recognized. The name given the enzyme was derived from its earliest recognized action, hence xanthine oxidase. Although little is known about the biological functions of the enzyme, the high affinity shown for xanthine and hypoxanthine may indicate that these are the most significant substrates biologically.

The Effects Of Herbicides On Three Soil Inhabiting Blue Green Algal Species, Lee C. Darlington

All Master's Theses

The object of this study was to examine the effects of varying concentrations of 2, 4-D, Dalapon, and Tordon on the growth of several nitrogen-fixing blue-green algal species obtained from soil samples and grown under culture conditions in the laboratory.

Shellfish Resources Of Washington, Robert Grant Levens

Graduate Student Research Papers

This paper will explore the salt water shellfish resources of Washington State. Particular emphasis will be placed upon the Pacific and Olympia Oysters, the Dungeness Crab, the research being done at Quilcene, and the lesser varieties of shellfish, including shrimp and the hardshell species of clams. The latter includes the littleneck, butter, Manila, and geoduc clams. A major emphasis will be the commercial importance of the shellfish, but the sportsman and the expanding role of Washington as a summer recreation area will not be overlooked.