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Full-Text Articles in Physical Sciences and Mathematics
The Effect Of Macromolecular Crowding On The Structure Of The Protein Complex Superoxide Dismutase, Ajith Rathnaweera Rajapaksha Mudalige
The Effect Of Macromolecular Crowding On The Structure Of The Protein Complex Superoxide Dismutase, Ajith Rathnaweera Rajapaksha Mudalige
Open Access Dissertations
Biological environments contain between 7 - 40% macromolecules by volume. This reduces the available volume for macromolecules and elevates the osmotic pressure relative to pure water. Consequently, biological macromolecules in their native environments tend to adopt more compact and dehydrated conformations than those in vitro. This effect is referred to as macromolecular crowding and constitutes an important physical difference between native biological environments and the simple solutions in which biomolecules are usually studied.^ We used small angle scattering (SAS) to measure the effects of macromolecular crowding on the size of a protein complex, superoxide dismutase (SOD). Crowding was induced using …
Consequences Of Short-Term Water Temperature Variability To Fish: Current And Future Climate Change Impacts, David P. Coulter
Consequences Of Short-Term Water Temperature Variability To Fish: Current And Future Climate Change Impacts, David P. Coulter
Open Access Dissertations
Water temperature has a profound influence on aquatic ectotherms by affecting all aspects of biological organization; from chemical and molecular functioning to whole-organism and population-level impacts. Natural processes and anthropogenic activities can create conditions where temperatures vary greatly through space and time. While exposure to temperature changes lasting ≥ 24 hours have been examined in some species, it is unclear how more rapid (< 24 hours; sub-daily) thermal fluctuations affect aquatic ectotherms. I used a combination of field observations, laboratory experiments, and modeling simulations to understand: 1) how habitat quality of aquatic ectotherms is affected in thermally dynamic environments; 2) the role of sub-daily temperature fluctuations on growth, survival, and stress responses in juvenile and adult fish; 3) how fish early life stages are affected by sub-daily temperature fluctuations; and 4) what impact thermally dynamic environments have on a model species at a population-level. A model quantifying habitat quality around power plant thermal discharges indicated that elevated and variable discharge temperatures affected habitat quality over a relatively small spatial area for aquatic ectotherms. Models examining elevated temperatures representing climate warming showed that the effects of industrial discharges and climate warming could have an interactive effect on habitat quality by increasing the spatial area and duration over which industrial thermal effluents impact aquatic ectotherms. Laboratory experiments indicated that closely related fishes can respond differently to the same sub-daily temperature fluctuations. Yellow perch (Perca flavescens) had higher consumption and growth under sub-daily temperature fluctuations but developed skin lesions; an indication of thermal stress. In contrast, these same fluctuations reduced growth in physiologically similar walleye (Sander vitreus) …
Novel Methods For Manipulating Ion Types In The Solution And Gas Phases For The Structural Analysis Of Biomolecules Using Mass Spectrometry, Christine M Fisher
Novel Methods For Manipulating Ion Types In The Solution And Gas Phases For The Structural Analysis Of Biomolecules Using Mass Spectrometry, Christine M Fisher
Open Access Dissertations
Mass Spectrometry has become a valuable tool for the analysis of a variety of molecules, making it applicable to many fields. The advent of nanoelectrospray ionization (nESI) as a soft/low energy ionization technique has enabled the analysis of large, intact biomolecules. Most mass spectrometry experiments consist of three main steps: ionization, probe step(s), and mass analysis. The present work focuses on a variety of methods for altering ion types at various stages of the mass spectrometry experiment to affect ion fragmentation. Ion types can be manipulated in the solution/droplet phases using novel nESI emitters, generated from borosilicate theta capillaries. These …
Quantitative Mrna Detection With Advanced Nonlinear Microscopy, Jing Liu
Quantitative Mrna Detection With Advanced Nonlinear Microscopy, Jing Liu
Open Access Dissertations
Cell-specific information on quantity and localization of key mRNA transcripts in single-cell level are critical to the assessment of cancer risk, therapy efficacy, and effective prevention strategies. While current techniques are not capable to visualize single mRNA transcript beyond the diffraction limit. In this thesis, two nonlinear technologies, second harmonic super-resolution microscopy (SHaSM) and transient absorption microscopy (TAM), are developed to detect and quantify single Human edimer receptor 2 (Her2) mRNA transcripts. The SHaSM is used to detect single mRNA transcript beyond the diffraction limit, while the TAM is employed to detect mRNA without the interference of fluorescence background. The …
Biology And Ecology Of Bigheaded Carp In An Invaded Ecosystem, Alison Adele Coulter
Biology And Ecology Of Bigheaded Carp In An Invaded Ecosystem, Alison Adele Coulter
Open Access Dissertations
Globally, the homogenization of species has become a threat to biodiversity. As species are transported around the world, a portion of these species, released intentionally or accidentally, may become invasive and can produce negative impacts. Great effort has been invested into early identification and prevention of invasions as these are considered less expensive than managing an invasion. Unfortunately, species may exhibit varying characteristics across ecosystems, and so their behavior and potential survival in a new environment may be difficult to predict. Therefore, I examined trends in the biology and behavior of invasive fishes, including the plasticity surrounding these and how …
Development Of Experimental And Instrumental Systems To Study Biological Systems, Amanda J Hemphill
Development Of Experimental And Instrumental Systems To Study Biological Systems, Amanda J Hemphill
Open Access Dissertations
Chapters 1-4 of this thesis describes the development of an experimental system to measure diffusion-limited reaction kinetics in a biological environment. About 100 years ago, the relationship between reaction rate and diffusion in homogenous solution, ie water or buffer, was described as a linear relationship by Smoluchowski. Applying this theory naively would suggest that since the diffusion coefficients drop by factors of 4-100 then the rates of reaction would drop by the same amount. However, recent theory and simulations suggest that this does not hold. Even though biological diffusion coefficients drop to 0.1-20% of that in buffer, these recent studies …