Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Drug delivery (2)
- 18O labeling (1)
- AMPA Receptors (1)
- Agroecology (1)
- Alpha-ketoglutarate dependent oxygenase (1)
-
- Antimicrobial (1)
- Antiobesity (1)
- Biochar (1)
- Biology (1)
- Biopharmaceuticals (1)
- CD163 (1)
- Calcium (1)
- Chemistry (1)
- Deep interactions (1)
- Electrospray ionization (1)
- Enzyme-substrate binding (1)
- Estimation (1)
- Factor Inhibiting HIF (1)
- Fluorescent Labeling (1)
- Haptoglobin (1)
- Hemoglobin (1)
- Hollow particles (1)
- Hydrogen sulfide (1)
- Hydrophobicity (1)
- Hydroxylase (1)
- Hypoxia Inducible Factor (1)
- ICP MS (1)
- Immunology (1)
- Ion exchange chromatography (1)
- Lipase inhibition (1)
Articles 1 - 9 of 9
Full-Text Articles in Physical Sciences and Mathematics
Utilizing In Silico And/Or Native Esi Approaches To Provide New Insights On Haptoglobin/Globin And Haptoglobin/Receptor Interactions, Ololade Fatunmbi
Utilizing In Silico And/Or Native Esi Approaches To Provide New Insights On Haptoglobin/Globin And Haptoglobin/Receptor Interactions, Ololade Fatunmbi
Doctoral Dissertations
Haptoglobin (Hp), an acute phase protein, binds free hemoglobin (Hb) dimers in one of the strongest non-covalent interactions known in biology. This interaction protects Hb from causing potentially severe oxidative damage and limiting nitric oxide bioavailability. Once Hb/Hp complexes are formed, they proceed to bind CD163, a cell surface receptor on macrophages leading to complex internalization and catabolism. Myoglobin, (Mb) a monomeric protein, that is normally found in the muscle but can be released into the blood in high concentrations during myocardial injury, is homologous to Hb and shares many conserved Hb/Hp interface residues. Both monomeric Hb and Mb species …
Estimation Problems In Complex Field Studies With Deep Interactions: Time-To-Event And Local Regression Models For Environmental Effects On Vital Rates, Krzysztof M. Sakrejda
Estimation Problems In Complex Field Studies With Deep Interactions: Time-To-Event And Local Regression Models For Environmental Effects On Vital Rates, Krzysztof M. Sakrejda
Doctoral Dissertations
Field studies that measure vital rates in context over extended time periods are a cornerstone of our understanding of population processes. These studies inform us about the relationship between biological process and environmental noise in an irreplaceable way. These data sets bring ``big data'' and ``big model'' challenges, which limit the application of standard software (e.g., \textbf{BUGS}). The environmental sensitivity of vital rates is also expected to exhibit interactions and non-linearity, which typically result in difficult model selection questions in large data sets. Finally, long-term ecological data sets often contain complex temporal structure. In commonly applied discrete-time models complex temporal …
Assessing Kiln-Produced Hardwood Biochar For Improving Soil Health In A Temperate Climate Agricultural Soil, Emily J. Cole
Assessing Kiln-Produced Hardwood Biochar For Improving Soil Health In A Temperate Climate Agricultural Soil, Emily J. Cole
Doctoral Dissertations
Soil quality has become a major factor used in assessing sustainable land management and the overall environmental quality, food security, and economic viability of agricultural lands. Recently, biochar has been touted as having many potential uses as a soil amendment for improving soil quality, specifically improving cation exchange capacity, pH and nutrient availability. However, soil biology also plays a significant role in biogeochemical processes that influence soil health and should be included in a more comprehensive study of soil health. This dissertation describes 4 projects within the same 3-year field study with the cumulative purpose of better understanding the effect …
Responsive Supramolecular Assemblies Based On Amphiphilic Polymers And Hybrid Materials, Longyu Li
Responsive Supramolecular Assemblies Based On Amphiphilic Polymers And Hybrid Materials, Longyu Li
Doctoral Dissertations
The design and synthesis of responsive supramolecular assemblies are of great interest due to their applications in a variety of areas such as drug delivery and sensing. We have developed a facile method to prepare self-crosslinking disulfide-based nanogels derived from an amphiphilic random copolymer containing a hydrophilic oligo-(ethylene glycol)-based side-chain functionality and a hydrophobic pyridyl disulfide functional group. This thesis first provides a concept of studying the influence of Hofmeister ions on the size and guest encapsulation stability of a polymeric nanogel. The size and core density of nanogel can be fine-tuned through the addition of both chaotropes and kosmotropes …
Chemical Biology-Based Probes For The Labeling Of Targets On Live Cells, Amanda M. Hussey
Chemical Biology-Based Probes For The Labeling Of Targets On Live Cells, Amanda M. Hussey
Doctoral Dissertations
Proper detection is the key to studying any processes on the cellular scale. Nowhere is this more evident than in the tight space which confines the synaptic cleft. Being able to ascertain the location of receptors on live neurons is fundamental to our understanding of not only how these receptors interact and move inside the cell but also how neurons function. Most detection methods rely on significantly altering the receptor; both tagging with a fluorescent protein or targeting the receptor by a fluorescent reporter in the form of a small molecule causes significant difficulties. These localization techniques often result in …
Inhibition And Cofactor Targeting Of Hypoxia-Sensing Proteins, Cornelius Y. Taabazuing
Inhibition And Cofactor Targeting Of Hypoxia-Sensing Proteins, Cornelius Y. Taabazuing
Doctoral Dissertations
Hypoxia Inducible Factor (HIF) is a transcription activator considered to be the main regulator of O2 homeostasis in humans. The transcriptional ability of HIF is regulated by the Fe2+/αKG-dependent enzyme, Factor Inhibiting HIF (FIH). FIH uses molecular oxygen to catalyze hydroxylation of an asparagine residue (Asn803) in the C-terminal transactivation domain (CTAD) of the HIFα subunit, abrogating HIF target gene expression. The mechanism of FIH and other αKG-dependent oxygenases involves the ordered sequential binding of αKG, substrate, and O2, which becomes activated to form a reactive ferryl intermediate that hydroxylates the substrate. The …
Transferrin-Based Therapeutics And Analytical Methods To Characterize Them, Son N. Nguyen
Transferrin-Based Therapeutics And Analytical Methods To Characterize Them, Son N. Nguyen
Doctoral Dissertations
Modern development of medicine requires detailed characterization by state-of-the art analytical techniques that can be used to analyze covalent structure, conformations and protein-receptor interaction to quantitatively measure biodistribution of protein therapeutics. Mass spectrometry has already become an indispensable tool facilitating all stages of protein drug development. Particularly, this work has demonstrated the tremendous potential of electrospray ionization (ESI) mass spectrometry (MS) in this arena by providing invaluable information beyond mass measurement that can be used to optimize protein drug conjugate structures during early stages of development, and to further catalyze drug design efforts. Additionally, a new sensitive and selective method …
Structure-Property Relationships At The Nano-Bio Interface: Engineering The Nanoparticle Surface For Immunomodulation, Daniel Fernando Moyano Marino
Structure-Property Relationships At The Nano-Bio Interface: Engineering The Nanoparticle Surface For Immunomodulation, Daniel Fernando Moyano Marino
Doctoral Dissertations
Each year, a variety of novel nanomaterials are being developed with the objective of treating different diseases. However, since nanomaterials are foreign to the human body, one of the principal factors that limit their use is the encounter with the first line of defense from the body: the immune system. If this interaction is not taken into account, an undesired recognition takes place and the efficiency of nanoparticle based therapies is dramatically reduced. As such, understanding the rules that govern this recognition is of prime importance in the field of nanomedicine. Following this line of thoughts (the driving force), the …
Physicochemical And Toxicological Assessment Of Antimicrobial Ε-Polylysine-Pectin Complexes, Cynthia L. Lopez Pena
Physicochemical And Toxicological Assessment Of Antimicrobial Ε-Polylysine-Pectin Complexes, Cynthia L. Lopez Pena
Doctoral Dissertations
ε-Polylysine is an appealing FDA-approved, all natural antimicrobial biopolymer effective against a wide range of microorganisms. Its implementation is greatly limited by its strong cationic charge, which has been linked to instability in food systems, perceived astringency and bitterness, and the ability to inhibit lipid digestion. Previous studies have shown that controlled complexation of ε-polylysine with anionic pectin is able to prevent instability and astringency in simplified model food systems, while maintaining the antimicrobial character of polylysine. Isothermal titration calorimetry, micro-electrophoresis, microscopy, and turbidity analyses of the stability of electrostatic pectin-polylysine complexes in the presence of strongly anionic κ-carrageenan, and …