Physical Sciences and Mathematics Commons^™

A Real Time Optical Biosensor Assay For Amoxicillin And Other Β-Lactams In Water Samples, Israel M. Scott, Lewis J. Kraft, Jonathan D. Parker, Kathryn Daniel, Sarah Kustick, Diana Kennen, Jonathan L. Mcmurry

Faculty and Research Publications

Antibiotic contamination of drinking water and sewage is a matter of environmental and public health concern. Traditionally, ELISA or HPLC methods have been used to detect and measure antibiotic contamination. By applying an optical biosensing method, biolayer inteferometry (BLI), we have developed a kinetic competition binding assay capable of quantitating less than lppm (~33 μM) amoxicillin. Similar to surface plasmon resonance, BLI senses changes that occur upon binding of one molecule to another near a surface to measure association and dissociation. Immobilized amoxicillin was used to screen for binding against an analyte solution of anti-amoxicillin equilibrated with amoxicillin-containing water samples, …