Physical Sciences and Mathematics Commons^™

The Coastal Monitor: Vol. 10 No. 2, John Tanacredi Ph.D.

The Coastal Monitor

Stephen J. Gould’s prophetic piece, “The Golden Rule: A Proper Scale for Our Environmental Crisis”, noted that, “Patience enjoys a long pedigree of favor”, which he elaborated, “usually involves a deep understanding of the fundamental principle… rarely grasped in daily life – the effects of scale.” Scientists observe changes incessantly, in dimensions and time, from microscopic conditions of cellular biology to the inconceivable distances of galaxies and their influences on Earth.

The Coastal Monitor: Vol. 10 No. 1, John Tanacredi Ph.D.

The Coastal Monitor

New Year’s Day has always been, for me, a time to reflect and project into the near future. Nature’s influence on us all certainly tops my listing with several environmental concerns. For example, the frequency and intensity of hurricanes to Long Island. A decade has passed since Superstorm Sandy re-opened the “Old Inlet” on Long Island which now has mostly naturally closed. So, the new year immediately prompts me to assess the previous year’s Hurricane Ian, a Category 4 system, with winds exceeding 150 mph, which had considerable impact in Florida, and then proceeded north resulting in over 2 inches …

Diverse Secondary Metabolites Are Expressed In Particle-Associated And Free-Living Microorganisms Of The Permanently Anoxic Cariaco Basin, David Gellar-Mcgrath, Paraskevi Mara, Gordon T. Taylor, Elizabeth A. Suter, Virginia Edgcomb, Maria Pachiadaki

Faculty Works: Biology, Chemistry, and Environmental Studies

Secondary metabolites play essential roles in ecological interactions and nutrient acquisition, and are of interest for their potential uses in medicine and biotechnology. Genome mining for biosynthetic gene clusters (BGCs) can be used for the discovery of new compounds. Here, we use metagenomics and metatranscriptomics to analyze BGCs in free-living and particle-associated microbial communities through the stratified water column of the Cariaco Basin, Venezuela. We recovered 565 bacterial and archaeal metagenome-assembled genomes (MAGs) and identified 1154 diverse BGCs. We show that differences in water redox potential and microbial lifestyle (particle-associated vs. free-living) are associated with variations in the predicted composition …

The Kappa Opioid Receptor: Candidate Pharmacotherapeutic Target For Multiple Sclerosis, Brian Reed, Surya Dutta

Faculty Works: Biology, Chemistry, and Environmental Studies

Multiple sclerosis (MS) afflicts millions of people worldwide. While multiple therapeutics have recently been developed and approved as treatment agents, they are not 100% effective. Recent developments investigating the endogenous opioid system involvement in MS has revealed that agonists of the kappa opioid receptor (KOR) have beneficial effects in both animal models of MS (and demyelinating disorders more generally) as well as in vitro models of remyelination. Several groups have contributed to this development. We summarize here the findings of these published studies, with comparisons of the effects and discussion of similarities and differences. The effects of KOR agonists involve …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained to assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 17 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Column letter “ 5. Record using “ Tallies “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Daily Meteorological Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Meteorological data is collected daily at CERCOM and is submitted to the National Weather Service (NWS), in Upton NY at the Brookhaven National Laboratories. Air Temperature, wind speed and direction, pH of precipitation, rain gauge, barometer pressure, cloud cover and estimated altitude by type, are data sets compiled and submitted to the NWS daily. CERCOM’s Co-Op site is the only continuous operating facility on Great South Bay.

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM conducts monitoring of 111 beach locations spanning from Brooklyn to Montauk during the optimal breeding season of Limulus polyphemus. These surveys span approximately 15 weeks, commencing in May and concluding in August. Each beach location is meticulously marked with GPS coordinates to ensure consistent annual revisitation. Participation in these surveys necessitates attendance at an HSC Survey training orientation, led by Mr. Key Maurelli, CERCOM's research assistant.

The Coastal Monitor: Vol. 9 No. 5, John Tanacredi Ph.D.

The Coastal Monitor

There are a multitude of lessons learned from the last 3 years of the Covid-19 pandemic. But one truly stands out ...the total lack of understanding of the basic chemistry associated with the nitrogen cycle, which in Suffolk County, NY, continues to be declared as the “evil-nitrogen”. The major source of nitrogen into the water body comes from the atmosphere which is the predominate constituent of the air we breathe79% nitrogen. Nitrogen compounds contribution to eutrophication in freshwater (ponds & lakes) systems is legendary and, the concentration of nitrogen in marine waters has always been known as a limiting agent …

Characterization Of Pyrrolidinyl-Hexahydro-Pyranopiperazines As A Novel Kappa Opioid Receptor Agonist Scaffold, Brian Reed, Michael Miller, Mayako Michino, Eduardo R. Butelman, Ariel Ben-Ezra, Philip Pikus, Michelle Morochnik, Yuli Kim, Amy Ripka, Joseph Vacca, Mary Jeanne Kreek

Faculty Works: Biology, Chemistry, and Environmental Studies

The kappa agonist structure–activity relationship around the novel, pyrrolidinyl substituted pyranopiperazine scaffold was developed. More specifically, the dichloroPhenylAcetamide-Pyrrolidinyl-PyranoPiperazine (PAPPP) core A was the focus of our work. The modulation of kappa receptor potency/G-protein activation and arrestin recruitment with respect to changes of the piperazine R group in A was demonstrated. Reduced β2-arrestin recruitment and differential G-protein bias were observed for select analogues. To better understand the subtlety in receptor signaling, analogues were profiled as the resolved enantiomers. To determine in vivo target engagement, a subset of compounds was tested in mice for stimulation of serum prolactin, a neuroendocrine biomarker of …

Changes In Breast Cancer Care In New York During The Covid-19 Pandemic, Alexandra Fiderlein, Cheyenne Rosado, Noelle L. Cutter Ph.D.

Faculty Works: Biology, Chemistry, and Environmental Studies

Breast cancer is the second most common malignancy among women in the United States. As such, the COVID-19 pandemic has caused medical facilities to change their methods of operation since March of 2020, including changes in diagnosis and treatment plans. New York (NY) has an unusually high incidence of breast cancer. This study analyzed the effects of the COVID-19 pandemic on breast cancer care (BCC) in NY. Women in NY that were diagnosed with or in remission for breast cancer were asked to take an online, anonymous survey regarding their BCC experience. For patients in treatment, 26% of women wished …

The Coastal Monitor: Vol. 8 No. 4, John Tanacredi Ph.D.

The Coastal Monitor

The original five acre of land portion of the historic Blue Points Oyster Company is located at the southern end of Atlantic Ave in West Sayville, Islip Town, Suffolk County, New York on the shore of Great South Bay. The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) is the science field station serving Molloy College’s BCES programs in Earth & Environmental Sciences and Biology. Our mission is to provide unique hands-on educational opportunities in the physical and life sciences, in collaboration with a host of Federal, State, and local and NGO groups, to bolster students’ career path. Great …

Eukaryotic Parasites Are Integral To A Productive Microbial Food Web In Oxygen-Depleted Waters, Elizabeth A. Suter, Maria Pachladaki, Gordon T. Taylor, Virginia P. Edgcomb

Faculty Works: Biology, Chemistry, and Environmental Studies

Oxygen-depleted water columns (ODWCs) host a diverse community of eukaryotic protists that change dramatically in composition over the oxic-anoxic gradient. In the permanently anoxic Cariaco Basin, peaks in eukaryotic diversity occurred in layers where dark microbial activity (chemoautotrophy and heterotrophy) were highest, suggesting a link between prokaryotic activity and trophic associations with protists. Using 18S rRNA gene sequencing, parasites and especially the obligate parasitic clade, Syndiniales, appear to be particularly abundant, suggesting parasitism is an important, but overlooked interaction in ODWC food webs. Syndiniales were also associated with certain prokaryotic groups that are often found in ODWCs, including Marinimicrobia and …

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during the optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May - August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation led by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the …

Daily Meteorological Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Meteorological data is collected daily at CERCOM and is submitted to the National Weather Service (NWS), in Upton NY at the Brookhaven National Laboratories. Air Temperature, wind speed and direction, pH of precipitation, rain gauge, barometer pressure, cloud cover and estimated altitude by type, are data sets compiled and submitted to the NWS daily. CERCOM’s Co-Op site is the only continuous operating facility on Great South Bay.

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Column letter “ 5. Record using “ Tallies “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained to assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 17 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries

The Bioinformatics Virtual Coordination Network: An Open-Source And Interactive Learning Environment, Benjamin J. Tully, Joy Buongiorno, Ashley B. Cohen, Jacob A. Cram, Arkadiy I. Garber, Sarah K. Hu, Arianna I. Krinos, Philip T. Leftwich, Alexis J. Marshall, Ella T. Sieradzki, Daan R. Speth, Elizabeth A. Suter, Christopher B. Trivedi, Luis E. Valentin-Alvarado, Jake L. Weissman, Bvcn Instructor Consortium

Faculty Works: Biology, Chemistry, and Environmental Studies

Lockdowns and “stay-at-home” orders, starting in March 2020, shuttered bench and field dependent research across the world as a consequence of the global COVID-19 pandemic. The pandemic continues to have an impact on research progress and career development, especially for graduate students and early career researchers, as strict social distance limitations stifle ongoing research and impede in-person educational programs. The goal of the Bioinformatics Virtual Coordination Network (BVCN) was to reduce some of these impacts by helping research biologists learn new skills and initiate computational projects as alternative ways to carry out their research. The BVCN was founded in April …

The Coastal Monitor: Vol. 7 No. 3, John Tanacredi Ph.D.

The Coastal Monitor

Where has all the pollution gone? No hazardous toxic algal blooms? Incredible summer estuarine water quality! No fish kills. Banner years in recreational fisheries for Stripers and Blues and incredible numbers of Humpback whales! No going to restaurants, so greater at home use of water and septic systems! Since March 2020, drier years than most, thus reducing runoff with fertilizers and organic pesticides, resulting in reduced water contamination. So maybe, just maybe, this COVID- 19 period which has altered our collective lives forever, has also resulted in a grand experiment on what has been hiding in plain sight? That is, …

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May- August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation lead by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the number of …

The Coastal Monitor Winter/Spring 2020 (Volume 6, No 2), John Tanacredi Ph.D.

The Coastal Monitor

It is a distinct pleasure to welcome our new President, Dr. James Lentini, who epitomizes the STEAM connection between Art and Science at Molloy College. Dr. Lentini has been a leader in higher education for more than 30 years and has had stellar academic training in the arts which include his passion for music. From composer, to classical guitarist, to music technology on an international level, receiving countless awards, honors, and praise from the most prestigious entities in the Arts. His transition to Molloy College was at the peak of the COVID-19 pandemic and his leadership, under these most difficult …

The Coastal Monitor Winter/Spring 2020, John Tanacredi Ph.D.

The Coastal Monitor

Throughout Long Island’s educational structure, STEM educational institutions have responded to the latest information from the Bureau of Labor Statistics who into our new year, have emphasized the fastest growing occupational categories over the next 10 years. These careers will be in the energy, health and education sectors, with the medical and technical sectors containing the highest paying occupations. Yet, hidden in the projected demand which will continue to spur on millions of new jobs, is corporate leaderships continuous claim that they cannot find qualified workers. This “skills gap” is due to a lack of strong cognitive and socio-economic skills. …

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May- August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation lead by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the number of …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor for dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained and assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 16 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries.

Salty Sensors, Fresh Ideas: The Use Of Molecular And Imaging Sensors In Understanding Plankton Dynamics Across Marine And Freshwater Ecosystems, Trisha Lyn Spanbauer, Christian Briseno-Avena, Kathleen Johnson Pitz, Elizabeth A. Suter

Faculty Works: Biology, Chemistry, and Environmental Studies

Understanding plankton dynamics in marine ecosystems has been advanced using in situ molecular and imaging instrumentation. A range of research objectives have been addressed through high‐resolution autonomous sampling, from food web characterization to harmful algal bloom dynamics. When used together, molecular and imaging sensors can cover the full‐size range, genetic identity, and life stages of plankton. Here, we briefly review a selection of in situ instrumentation developed for the collection of molecular and imaging information on plankton communities in marine ecosystems. In addition, we interviewed a selection of instrumentation developers to determine if the transfer of sensor technology from marine …

The Coastal Monitor Fall 2019, John Tanacredi Ph.D.

The Coastal Monitor

In 2013 I had the distinct pleasure to be offered the opportunity to become a part of the Molloy College family. President Dr. Drew Bogner believed that CERCOM would be an important part of building an exciting program for students in Earth and Environmental Science at Molloy. Dr. Bogner was able to envision how the CERCOM Field Station, would enrich Molloy College science students, given its decades of earth, environmental, and marine sciences research. He had the foresight to understand that the experiences at CERCOM would help foster a vast array of international academic collaborations which now include 14 countries. …

The Presence Of Above Standard Levels Of Commonly Tested Contaminants In Communiites On Long Island, New York: The Impact Of Income On Untreated Water Quality, Amy Catalano, Michael A. Marino Ed.D.

Faculty Works: Biology, Chemistry, and Environmental Studies

Water quality is a pressing issue in many communities. Long Island (LI), New York, rests on a system of aquifers created by prehistoric glacial activity. These aquifer systems are the only source of drinking water for LI. Water quality issues are pervasive in the region due to nitrate pollution, caused by antiquated septic systems in much of the Island, as well as the prevalence of environmental clean-up sites. Using the Watertraq database, we searched for levels of select compounds that were present in wells above acceptable levels on LI. We then collected demographic data from the U.S. Census, including income, …

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.