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Enhanced Biotransformation Of Carbon Tetrachloride By Acetobacterium Woodii Upon Addition Of Hydroxocobalamin And Fructose, Syed A. Hashsham, David L. Freedman

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The objective of this study was to evaluate the effect of hydroxocobalamin (OH-Cbl) on transformation of high concentrations of carbon tetrachloride (CT) by Acetobacterium woodii (ATCC 29683). Complete transfor-mation of 470 􀀀M (72 mg/liter [aqueous]) CT was achieved by A. woodii within 2.5 days, when 10 􀀀M OH-Cbl was added along with 25.2 mM fructose. This was approximately 30 times faster than A. woodii cultures (live or autoclaved) and medium that did not receive OH-Cbl and 5 times faster than those controls that did receive OH-Cbl, but either live A. woodii or fructose was missing. CT transformation in treatments with …