Physical Sciences and Mathematics Commons^™

Assessment Of The Occurrence And Potential Risks Of Antibiotics And Their Metabolites In South Florida Waters Using Liquid Chromatography Tandem Mass Spectrometry, Venkata Reddy Panditi

FIU Electronic Theses and Dissertations

An automated on-line SPE-LC-MS/MS method was developed for the quantitation of multiple classes of antibiotics in environmental waters. High sensitivity in the low ng/L range was accomplished by using large volume injections with 10-mL of sample. Positive confirmation of analytes was achieved using two selected reaction monitoring (SRM) transitions per antibiotic and quantitation was performed using an internal standard approach. Samples were extracted using online solid phase extraction, then using column switching technique; extracted samples were immediately passed through liquid chromatography and analyzed by tandem mass spectrometry. The total run time per each sample was 20 min. The statistically calculated …