Physical Sciences and Mathematics Commons^™

6d Single-Fluorogen Orientation-Localization Microscopy For Elucidating The Architecture Of Beta-Sheet Assemblies And Biomolecular Condensates, Tingting Wu, Weiyan Zhou, Jai S. Rudra, Rohit V. Pappu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We develop six-dimensional single-molecule orientation-localization microscopy (SMOLM) to measure the 3D positions and 3D orientations simultaneously of single fluorophores. We show how careful optimization of phase and polarization modulation components can encode phase, polarization, and angular spectrum information from each fluorescence photon into a microscope’s dipole-spread function. We used the transient binding and blinking of Nile red (NR) to characterize the helical structure of fibrils formed by designed amphipathic peptides, KFE8^L and KFE8^D, and the pathological amyloid-beta peptide Aβ42. We also deployed merocyanine 540 to uncover the interfacial architectures of biomolecular condensates.

Six-Dimensional Single-Molecule Imaging With Isotropic Resolution Using A Multi-View Reflector Microscope, Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Imaging of both the positions and orientations of single fluorophores, termed single-molecule orientation-localization microscopy, is a powerful tool for the study of biochemical processes. However, the limited photon budget associated with single-molecule fluorescence makes high-dimensional imaging with isotropic, nanoscale spatial resolution a formidable challenge. Here we realize a radially and azimuthally polarized multi-view reflector (raMVR) microscope for the imaging of the three-dimensional (3D) positions and 3D orientations of single molecules, with precisions of 10.9 nm and 2.0° over a 1.5-μm depth range. The raMVR microscope achieves 6D super-resolution imaging of Nile red molecules transiently bound to lipid-coated spheres, accurately resolving …

Resolving The Three-Dimensional Rotational And Translational Dynamics Of Single Molecules Using Radially And Azimuthally Polarized Fluorescence, Oumeng Zhang, Weiyan Zhou, Jin Lu, Tingting Wu, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We report a radially and azimuthally polarized (raPol) microscope for high detection and estimation performance in single-molecule orientation-localization microscopy (SMOLM). With 5000 photons detected from Nile red (NR) transiently bound within supported lipid bilayers (SLBs), raPol SMOLM achieves 2.9 nm localization precision, 1.5° orientation precision, and 0.17 sr precision in estimating rotational wobble. Within DPPC SLBs, SMOLM imaging reveals the existence of randomly oriented binding pockets that prevent NR from freely exploring all orientations. Treating the SLBs with cholesterol-loaded methyl-β-cyclodextrin (MβCD-chol) causes NR’s orientational diffusion to be dramatically reduced, but curiously NR’s median lateral displacements drastically increase from 20.8 to …

Single-Molecule Localization Microscopy Of 3d Orientation And Anisotropic Wobble Using A Polarized Vortex Point Spread Function, Tianben Ding, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Within condensed matter, single fluorophores are sensitive probes of their chemical environments, but it is difficult to use their limited photon budget to image precisely their positions, 3D orientations, and rotational diffusion simultaneously. We demonstrate the polarized vortex point spread function (PSF) for measuring these parameters, including characterizing the anisotropy of a molecule’s wobble, simultaneously from a single image. Even when imaging dim emitters (∼500 photons detected), the polarized vortex PSF can obtain 12 nm localization precision, 4°–8° orientation precision, and 26° wobble precision. We use the vortex PSF to measure the emission anisotropy of fluorescent beads, the wobble dynamics …

Computational Modelling Enables Robust Multidimensional Nanoscopy, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

The following sections are included:

• Present State of Computational Modelling in Fluorescence Nanoscopy

• Recent Contributions to Computational Modelling in Fluorescence Nanoscopy

• Outlook on Computational Modelling in Fluorescence Nanoscopy

• Acknowledgments

• References

Single‐Molecule 3d Orientation Imaging Reveals Nanoscale Compositional Heterogeneity In Lipid Membranes, Jin Lu, Hesam Mazidi, Tianben Ding, Oumeng Zhang, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

In soft matter, thermal energy causes molecules to continuously translate and rotate, even in crowded environments, thereby impacting the spatial organization and function of most molecular assemblies, such as lipid membranes. Directly measuring the orientation and spatial organization of large collections (>3000 molecules μm⁻²) of single molecules with nanoscale resolution remains elusive. In this paper, we utilize SMOLM, single‐molecule orientation localization microscopy, to directly measure the orientation spectra (3D orientation plus “wobble”) of lipophilic probes transiently bound to lipid membranes, revealing that Nile red's (NR) orientation spectra are extremely sensitive to membrane chemical composition. SMOLM images resolve …

Elicitation And Aggregation Of Data In Knowledge Intensive Crowdsourcing, Dohoon Kim

All Computer Science and Engineering Research

With the significant advance of internet and connectivity, crowdsourcing gained more popularity and various crowdsourcing platforms emerged. This project focuses on knowledge-intensive crowdsourcing, in which agents are presented with the tasks that require certain knowledge in domain. Knowledge-intensive crowdsourcing requires agents to have experiences on the specific domain. With the constraint of resources and its trait as sourcing from crowd, platform is likely to draw agents with different levels of expertise and knowledge and asking same task can result in bad performance. Some agents can give better information when they are asked with more general question or more knowledge-specific task …

A Virtual 4d Ct Scanner, Xiwen Li

All Computer Science and Engineering Research

4D CT scan is widely used in medical imaging. Images are acquired through phases. In this case, we can track the motion of organs such as heart. However, it also introduces motion artifacts. A lot of research focuses on remove these artifacts. It is difficult to acquire artifact data by a real CT scanner. In this project, we implement a virtual CT machine to simulate the real 4D CT scan. we also conduct experi- ments to check its clinical reality with respect to respiratory and heart motion parameters.

Centrality Of Blockchain, Zixuan Li

All Computer Science and Engineering Research

Decentralization is widely recognized as the property and one of most important advantage of blockchain over legacy systems. However, decentralization is often discussed on the consensus layer and recent research shows the trend of centralization on several subsystem of blockchain. In this project, we measured centralization of Bitcoin and Ethereum on source code, development eco-system, and network node levels. We found that the programming language of project is highly centralized, code clone is very common inside Bitcoin and Ethereum community, and developer contribution distribution is highly centralized. We further discuss how could these centralizations lead to security issues in blockchain. …

Solving Disappearance At Gastech With Visual Analytic Techniques, Saulet Yskak

All Computer Science and Engineering Research

We are living in a society, where images and charts speak louder than words. Therefore, information visualization plays a major role in solving complex problems since it provides a visual summary of data that makes it easier to identify trends and patterns.

In this master project, I propose a web – based visual analytics tool that enables to analyze complex email and time based / event series data. The visual analytics framework uses test data from IEEE VAST Challenge 2014: Mini challenge 1 that concentrated on the disappearance of employees of a fictional GAStech company, but the tool allows users …

Measuring Localization Confidence For Quantifying Accuracy And Heterogeneity In Single-Molecule Super-Resolution Microscopy, Hesam Mazidi, Tianben Ding, Arye Nehorai, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We present a computational method, termed Wasserstein-induced flux (WIF), to robustly quantify the accuracy of individual localizations within a single-molecule localization microscopy (SMLM) dataset without ground- truth knowledge of the sample. WIF relies on the observation that accurate localizations are stable with respect to an arbitrary computational perturbation. Inspired by optimal transport theory, we measure the stability of individual localizations and develop an efficient optimization algorithm to compute WIF. We demonstrate the advantage of WIF in accurately quantifying imaging artifacts in high-density reconstruction of a tubulin network. WIF represents an advance in quantifying systematic errors with unknown and complex distributions, …

A Computationally-Efficient Bound For The Variance Of Measuring The Orientation Of Single Molecules, Tingting Wu, Tianben Ding, Hesam Mazidi, Oumeng Zhang, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Modulating the polarization of excitation light, resolving the polarization of emitted fluorescence, and point spread function (PSF) engineering have been widely leveraged for measuring the orientation of single molecules. Typically, the performance of these techniques is optimized and quantified using the Cramér-Rao bound (CRB), which describes the best possible measurement variance of an unbiased estimator. However, CRB is a local measure and requires exhaustive sampling across the measurement space to fully characterize measurement precision. We develop a global variance upper bound (VUB) for fast quantification and comparison of orientation measurement techniques. Our VUB tightly bounds the diagonal elements of the …

Nanoscale Colocalization Of Fluorogenic Probes Reveals The Role Of Oxygen Vacancies In The Photocatalytic Activity Of Tungsten Oxide Nanowires, Meikun Shen, Tianben Ding, Steven T. Hartman, Fudong Wang, Christina Krucylak, Zheyu Wang, Che Tan, Bo Yin, Rohan Mishra, Matthew D. Lew, Bryce Sadtler

Electrical & Systems Engineering Publications and Presentations

Defect engineering is a strategy that has been widely used to design active semiconductor photocatalysts. However, understanding the role of defects, such as oxygen vacancies, in controlling photocatalytic activity remains a challenge. Here, we report the use of chemically triggered fluorogenic probes to study the spatial distribution of active regions in individual tungsten oxide nanowires using super-resolution fluorescence microscopy. The nanowires show significant heterogeneity along their lengths for the photocatalytic generation of hydroxyl radicals. Through quantitative, coordinate-based colocalization of multiple probe molecules activated by the same nanowires, we demonstrate that the nanoscale regions most active for the photocatalytic generation of …

The Effects Of Mixed-Initiative Visualization Systems On Exploratory Data Analysis, Alvitta Ottley, Adam Kern

All Computer Science and Engineering Research

The primary purpose of information visualization is to act as a window between a user and the data. Historically, this has been accomplished via a single-agent framework: the only decision-maker in the relationship between visualization system and analyst is the analyst herself. Yet this framework arose not from first principles, but a necessity. Before this decade, computers were limited in their decision-making capabilities, especially in the face of large, complex datasets and visualization systems. This paper aims to present the design and evaluation of a mixed-initiative system that aids the user in handling large, complex datasets and dense visualization systems. …

Point Cloud Processing With Neural Networks, Stephanie Miller, Jiahao Li

All Computer Science and Engineering Research

In this project, we explore new techniques and architectures for applying deep neural networks when the input is point cloud data. We first consider applying convolutions on regular pixel and voxel grids, using polynomials of point coordinates and Fourier transforms to get a rich feature representation for all points mapped to the same pixel or voxel. We also apply these ideas to generalize the recently proposed "interpolated convolution", by learning continuous-space kernels as a combination of polynomial and Fourier basis kernels. Experiments on the ModelNet40 dataset demonstrate that our methods have superior performance over the baselines in 3D object recognition.

Static Taint Analysis Of Binary Executables Using Architecture-Neutral Intermediate Representation, Elaine Cole

All Computer Science and Engineering Research

Ghidra, National Security Agency’s powerful reverse engineering framework, was recently released open-source in April 2019 and is capable of lifting instructions from a wide variety of processor architectures into its own register transfer language called p-code. In this project, we present a new tool which leverages Ghidra’s specific architecture-neutral intermediate representation to construct a control flow graph modeling all program executions of a given binary and apply static taint analysis. This technique is capable of identifying the information flow of malicious input from untrusted sources that may interact with key sinks or parts of the system without needing access to …

Pipelined Parallelism In A Work-Stealing Scheduler, Thomas Kelly

All Computer Science and Engineering Research

A pipeline is a particular type of parallel program structure, often used to represent loops with cross-iteration dependencies. Pipelines cannot be expressed with the typical parallel language constructs offered by most environments. Therefore, in order to run pipelines, it is necessary to write a parallel language and scheduler with specialized support for them. Some such schedulers are written exclusively for pipelines and unable to run any other type of program, which allows for certain optimizations that take advantage of the pipeline structure. Other schedulers implement support for pipelines on top of a general-purpose scheduling algorithm. One example of such an …

Erratum: "Imaging The Three‐Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri‐Spot Point Spread Function", Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

In the original paper, a calibration error exists in the image-formation model used to analyze experimental images taken by our microscope, causing a bias in the orientation measurements in Figs. 2 and 3. The updated measurements are shown in Fig. E1. We have also updated the supplementary material for the original article to discuss the revised PSF model and estimation algorithms (supplementary material 2) and show the revised model and measurements (Figs. S1, S3, S7, S8, and S10–S13).

Smart Home Audio Assistant, Xipeng Wang

All Computer Science and Engineering Research

This report introduces an audio processing algorithm. It provides a way to access smart devices using audio. Although there are many audio assistants already on the market, most of them will not be able to control the smart devices. Therefore, this new system presented in this report will provide a way to analysis the customer’s questions. Then the algorithm will be able to query smart device information, modify the schedule or provide the reason for some arrangement.

A Survey On The Role Of Individual Differences On Visual Analytics Interactions: Masters Project Report, Jesse Huang, Alvitta Ottley

All Computer Science and Engineering Research

There is ample evidence in the visualization commu- nity that individual differences matter. These prior works high- light various traits and cognitive abilities that can modulate the use of the visualization systems and demonstrate a measurable influence on speed, accuracy, process, and attention. Perhaps the most important implication of this body of work is that we can use individual differences as a mechanism for estimating people’s potential to effectively leverage visual interfaces or to identify those people who may struggle. As visual literacy and data fluency continue to become essential skills for our everyday lives, we must embrace the growing …

Challenges In Integrating Iot In Smart Home, Leiquan Pan, Chenyang Lu

All Computer Science and Engineering Research

Wireless devices have become a major part in Smart Home industry. Almost every smart home company has its own wireless solutions and cloud services. Normally, customers can only monitor and control smart devices through applications or platforms companies provided. It causes inconveniences and problems when we have lots of smart devices. In my master project, I did two projects to implement smart home IoT applications. From a single functionality IoT application to a more complicated smart home system, there are lots of challenges and problems appeared. This article will mainly focus on challenges in integrating IoT in a smart home.

Feature Extraction Form Ct Scan Of Plant Root, Chunyuan Li

All Computer Science and Engineering Research

Roots are vital for plant by absorbing water and nutrients and providing anchorage from beneath the soil. These roles are closely related to the roots’ architecture, which describes the geometry of individual roots and their branching structure. We proposed a pipeline to efficiently annotate root architecture. My contribution focus on building an interactive tool to visual and annotate root architecture. Besides, we come up with heuristics to automate the annotation process.

Computational Geometry Teaching Tool, Yujie Zhou, Tao Ju

All Computer Science and Engineering Research

When students are taking Computational Geometry course which covers many geometry algorithms, most of them are difficult to follow because these algorithms are very abstract even if authors draw pictures to illustrate. In order to help students to get a better understanding of these algorithms, we decide to design Computational Geometry Teaching Tool. This tool is a web application that covers 8 geometry algorithms : Graham Scan, Quick Hull, Line Segment Intersection, Dual, Line Arrangement, Voronoi Diagram, Incremental Delaunay Triangulation and Kd Tree. First, this tool is developed by using JavaScript so that users don't need to install any software …

Fundamental Limits Of Measuring Single-Molecule Rotational Mobility, Oumeng Zhang, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.

Security Services Using Blockchains: A State Of The Art Survey, Maeda Zolanvari, Aiman Erbad, Raj Jain, Mohammed Samaka

All Computer Science and Engineering Research

This article surveys blockchain-based approaches for several security services. These services include authentication, confidentiality, privacy and access control list (ACL), data and resource provenance, and integrity assurance. All these services are critical for the current distributed applications, especially due to the large amount of data being processed over the networks and the use of cloud computing. Authentication ensures that the user is who he/she claims to be. Confidentiality guarantees that data cannot be read by unauthorized users. Privacy provides the users the ability to control who can access their data. Provenance allows an efficient tracking of the data and resources …

Super‐Resolution Imaging Of Amyloid Structures Over Extended Times By Using Transient Binding Of Single Thioflavin T Molecules, Kevin Spehar, Tianben Ding, Yuanzi Sun, Niraja Kedia, Jin Lu, George R. Nahass, Matthew D. Lew, Jan Bieschke

Electrical & Systems Engineering Publications and Presentations

Oligomeric amyloid structures are crucial therapeutic targets in Alzheimer's and other amyloid diseases. However, these oligomers are too small to be resolved by standard light microscopy. We have developed a simple and versatile tool to image amyloid structures by using thioflavin T without the need for covalent labeling or immunostaining. The dynamic binding of single dye molecules generates photon bursts that are used for fluorophore localization on a nanometer scale. Thus, photobleaching cannot degrade image quality, allowing for extended observation times. Super‐resolution transient amyloid binding microscopy promises to directly image native amyloid by using standard probes and record amyloid dynamics …

Imaging The Three-Dimensional Orientation And Rotational Mobility Of Fluorescent Emitters Using The Tri-Spot Point Spread Function, Oumeng Zhang, Jin Lu, Tianben Ding, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

Fluorescence photons emitted by single molecules contain rich information regarding their rotational motions, but adapting single-molecule localization microscopy (SMLM) to measure their orientations and rotational mobilities with high precision remains a challenge. Inspired by dipole radiation patterns, we design and implement a Tri-spot point spread function (PSF) that simultaneously measures the three-dimensional orientation and the rotational mobility of dipole-like emitters across a large field of view. We show that the orientation measurements done using the Tri-spot PSF are sufficiently accurate to correct the anisotropy-based localization bias, from 30 nm to 7 nm, in SMLM. We further characterize the emission anisotropy …

Measuring 3d Molecular Orientation And Rotational Mobility Using A Tri-Spot Point Spread Function, Oumeng Zhang, Tianben Ding, Jin Lu, Hesam Mazidi, Matthew D. Lew

Electrical & Systems Engineering Publications and Presentations

We present a method to measure the molecular orientation and rotational mobility of single-molecule emitters by designing and implementing a Tri-spot point spread function. It can measure all degrees of freedom related to molecular orientation and rotational mobility. Its design is optimized by maximizing the theoretical limit of its measurement precision. We evaluate the precision and accuracy of the Tri-spot PSF by measuring the orientation and effective rotational mobility of single fluorescent molecules embedded in a polymer matrix.

Decoupling Information And Connectivity In Information-Centric Networking, Hila Ben Abraham, Jyoti Parwatikar, John Dehart, Adam Drescher, Patrick Crowley

All Computer Science and Engineering Research

This paper introduces and demonstrates the concept of Information-Centric Transport as a mechanism for cleanly decoupling the information plane from the connectivity plane in Information-Centric Networking (ICN) architectures, such as NDN and CICN. These are coupled in today's incarnations of NDN and CICN through the use of forwarding strategy, which is the architectural component for deciding how to forward packets in the presence of either multiple next-hop options or dynamic feedback. As presently designed, forwarding strategy is not sustainable: application developers can only confidently specify strategy if they understand connectivity details, while network node operators can only confidently assign strategies …

Multipath And Rate Stability, Junjie Liu, Roch A. Guérin

All Computer Science and Engineering Research

Originally Published In Proc. IEEE Globecom Conference - CQRM: Communication QoS, Reliability & Modeling Symposium