Physical Sciences and Mathematics Commons^™

Diverse Secondary Metabolites Are Expressed In Particle-Associated And Free-Living Microorganisms Of The Permanently Anoxic Cariaco Basin, David Gellar-Mcgrath, Paraskevi Mara, Gordon T. Taylor, Elizabeth A. Suter, Virginia Edgcomb, Maria Pachiadaki

Faculty Works: Biology, Chemistry, and Environmental Studies

Secondary metabolites play essential roles in ecological interactions and nutrient acquisition, and are of interest for their potential uses in medicine and biotechnology. Genome mining for biosynthetic gene clusters (BGCs) can be used for the discovery of new compounds. Here, we use metagenomics and metatranscriptomics to analyze BGCs in free-living and particle-associated microbial communities through the stratified water column of the Cariaco Basin, Venezuela. We recovered 565 bacterial and archaeal metagenome-assembled genomes (MAGs) and identified 1154 diverse BGCs. We show that differences in water redox potential and microbial lifestyle (particle-associated vs. free-living) are associated with variations in the predicted composition …

The Kappa Opioid Receptor: Candidate Pharmacotherapeutic Target For Multiple Sclerosis, Brian Reed, Surya Dutta

Faculty Works: Biology, Chemistry, and Environmental Studies

Multiple sclerosis (MS) afflicts millions of people worldwide. While multiple therapeutics have recently been developed and approved as treatment agents, they are not 100% effective. Recent developments investigating the endogenous opioid system involvement in MS has revealed that agonists of the kappa opioid receptor (KOR) have beneficial effects in both animal models of MS (and demyelinating disorders more generally) as well as in vitro models of remyelination. Several groups have contributed to this development. We summarize here the findings of these published studies, with comparisons of the effects and discussion of similarities and differences. The effects of KOR agonists involve …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained to assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 17 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Column letter “ 5. Record using “ Tallies “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Daily Meteorological Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Meteorological data is collected daily at CERCOM and is submitted to the National Weather Service (NWS), in Upton NY at the Brookhaven National Laboratories. Air Temperature, wind speed and direction, pH of precipitation, rain gauge, barometer pressure, cloud cover and estimated altitude by type, are data sets compiled and submitted to the NWS daily. CERCOM’s Co-Op site is the only continuous operating facility on Great South Bay.

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM conducts monitoring of 111 beach locations spanning from Brooklyn to Montauk during the optimal breeding season of Limulus polyphemus. These surveys span approximately 15 weeks, commencing in May and concluding in August. Each beach location is meticulously marked with GPS coordinates to ensure consistent annual revisitation. Participation in these surveys necessitates attendance at an HSC Survey training orientation, led by Mr. Key Maurelli, CERCOM's research assistant.

Characterization Of Pyrrolidinyl-Hexahydro-Pyranopiperazines As A Novel Kappa Opioid Receptor Agonist Scaffold, Brian Reed, Michael Miller, Mayako Michino, Eduardo R. Butelman, Ariel Ben-Ezra, Philip Pikus, Michelle Morochnik, Yuli Kim, Amy Ripka, Joseph Vacca, Mary Jeanne Kreek

Faculty Works: Biology, Chemistry, and Environmental Studies

The kappa agonist structure–activity relationship around the novel, pyrrolidinyl substituted pyranopiperazine scaffold was developed. More specifically, the dichloroPhenylAcetamide-Pyrrolidinyl-PyranoPiperazine (PAPPP) core A was the focus of our work. The modulation of kappa receptor potency/G-protein activation and arrestin recruitment with respect to changes of the piperazine R group in A was demonstrated. Reduced β2-arrestin recruitment and differential G-protein bias were observed for select analogues. To better understand the subtlety in receptor signaling, analogues were profiled as the resolved enantiomers. To determine in vivo target engagement, a subset of compounds was tested in mice for stimulation of serum prolactin, a neuroendocrine biomarker of …

Changes In Breast Cancer Care In New York During The Covid-19 Pandemic, Alexandra Fiderlein, Cheyenne Rosado, Noelle L. Cutter Ph.D.

Faculty Works: Biology, Chemistry, and Environmental Studies

Breast cancer is the second most common malignancy among women in the United States. As such, the COVID-19 pandemic has caused medical facilities to change their methods of operation since March of 2020, including changes in diagnosis and treatment plans. New York (NY) has an unusually high incidence of breast cancer. This study analyzed the effects of the COVID-19 pandemic on breast cancer care (BCC) in NY. Women in NY that were diagnosed with or in remission for breast cancer were asked to take an online, anonymous survey regarding their BCC experience. For patients in treatment, 26% of women wished …

Eukaryotic Parasites Are Integral To A Productive Microbial Food Web In Oxygen-Depleted Waters, Elizabeth A. Suter, Maria Pachladaki, Gordon T. Taylor, Virginia P. Edgcomb

Faculty Works: Biology, Chemistry, and Environmental Studies

Oxygen-depleted water columns (ODWCs) host a diverse community of eukaryotic protists that change dramatically in composition over the oxic-anoxic gradient. In the permanently anoxic Cariaco Basin, peaks in eukaryotic diversity occurred in layers where dark microbial activity (chemoautotrophy and heterotrophy) were highest, suggesting a link between prokaryotic activity and trophic associations with protists. Using 18S rRNA gene sequencing, parasites and especially the obligate parasitic clade, Syndiniales, appear to be particularly abundant, suggesting parasitism is an important, but overlooked interaction in ODWC food webs. Syndiniales were also associated with certain prokaryotic groups that are often found in ODWCs, including Marinimicrobia and …

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during the optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May - August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation led by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the …

Daily Meteorological Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Meteorological data is collected daily at CERCOM and is submitted to the National Weather Service (NWS), in Upton NY at the Brookhaven National Laboratories. Air Temperature, wind speed and direction, pH of precipitation, rain gauge, barometer pressure, cloud cover and estimated altitude by type, are data sets compiled and submitted to the NWS daily. CERCOM’s Co-Op site is the only continuous operating facility on Great South Bay.

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Column letter “ 5. Record using “ Tallies “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained to assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 17 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries

The Bioinformatics Virtual Coordination Network: An Open-Source And Interactive Learning Environment, Benjamin J. Tully, Joy Buongiorno, Ashley B. Cohen, Jacob A. Cram, Arkadiy I. Garber, Sarah K. Hu, Arianna I. Krinos, Philip T. Leftwich, Alexis J. Marshall, Ella T. Sieradzki, Daan R. Speth, Elizabeth A. Suter, Christopher B. Trivedi, Luis E. Valentin-Alvarado, Jake L. Weissman, Bvcn Instructor Consortium

Faculty Works: Biology, Chemistry, and Environmental Studies

Lockdowns and “stay-at-home” orders, starting in March 2020, shuttered bench and field dependent research across the world as a consequence of the global COVID-19 pandemic. The pandemic continues to have an impact on research progress and career development, especially for graduate students and early career researchers, as strict social distance limitations stifle ongoing research and impede in-person educational programs. The goal of the Bioinformatics Virtual Coordination Network (BVCN) was to reduce some of these impacts by helping research biologists learn new skills and initiate computational projects as alternative ways to carry out their research. The BVCN was founded in April …

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May- August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation lead by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the number of …

The Study For Grassland Nutrition Of Typical Grasslands Difference Degradation Grade, Yushan Jia

IGC Proceedings (1997-2023)

No abstract provided.

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from May- August. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation lead by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the number of …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor for dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained and assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 16 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries.

Salty Sensors, Fresh Ideas: The Use Of Molecular And Imaging Sensors In Understanding Plankton Dynamics Across Marine And Freshwater Ecosystems, Trisha Lyn Spanbauer, Christian Briseno-Avena, Kathleen Johnson Pitz, Elizabeth A. Suter

Faculty Works: Biology, Chemistry, and Environmental Studies

Understanding plankton dynamics in marine ecosystems has been advanced using in situ molecular and imaging instrumentation. A range of research objectives have been addressed through high‐resolution autonomous sampling, from food web characterization to harmful algal bloom dynamics. When used together, molecular and imaging sensors can cover the full‐size range, genetic identity, and life stages of plankton. Here, we briefly review a selection of in situ instrumentation developed for the collection of molecular and imaging information on plankton communities in marine ecosystems. In addition, we interviewed a selection of instrumentation developers to determine if the transfer of sensor technology from marine …

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ Take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Daily Meteorological Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Meteorological Methodologies and Equipment: - Davis 6152 Wireless Vantage Pro2 Weather Station - USGS Tide Gauge - NOAA Rain (precipitation) Gauge - Hand held salt water thermometer - Yard stick - NOAA anemometer CERCOM’s Research Assistant Mr. Kyle Maurelli is responsible for maintenance of co-op NWS weather station at CERCOM and submission of daily weather data to NOAA-NWS database 365 days per year.

Long Island Horseshoe Crab Network Annual Inventory Report, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM monitors 115 beach locations from Brooklyn – Montauk during optimal Limulus Polyphemus breeding season. These beach surveys take place for approximately 15 weeks, ranging from MayAugust. These beach locations are marked specifically using GPS coordinates, to assure the same location is visited each year. Students and volunteers are required to attend a HSC Survey training orientation lead by CERCOM’s research assistant, Mr. Kyle Maurelli. Surveying is done around tide and moon cycles (New/Full Moon). Each site takes about 1-2 hours and data is recorded along an approximate 1 mile distance. Walking along the water’s edge the number of male …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 11 locations in the Great South Bay from Memorial Day – Labor Day to monitor for dissolved oxygen (DO), pH, salinity, clarity and temperature. Students are trained and assist in CERCOM’s water quality data collection by research assistant Mr. Kyle Maurelli. This monitoring program has been conducted for the past 16 years. These parameters are critical in determining long term water quality conditions in Long Island estuaries.

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

CERCOM visits 11 locations in Great South Bay to monitor dissolved oxygen (DO), pH, salinity, clarity, and temperature. This monitoring program has been conducted for the past 20 years. These parameters are critical in determining long-term water quality conditions in Long Island estuaries.

Great South Bay, Long Island, New York Summer Phytoplankton Identification & Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Kyle F. Maurelli

CERCOM Reports

Phytoplankton Collection Methodologies: 80 micron Plankton Tow Net with sample bottle attachment

Phytoplankton Protocol: 1.Gather Samples 2.Make one slide per sample 3.View slides using microscope connected with computer 4. Record findings using “ Row # “ and “ Colum letter “ 5. Record using “ Tally’s “ per species found within sample 6. Capture anything interesting “ take Picture “ 7.Duplicate pictures taken 8.Make sure measurement of species found is taken 9. Email Jennifer Maucher at Jennifer.maucher@noaa.gov , include pictures, questions and names of the species you “guess” you found.

Single-Cell Growth Rates In Photoautotrophic Populations Measured By Stable Isotope Probing And Resonance Raman Microspectrometry, Gordon T. Taylor, Elizabeth A. Suter, Zhuo Q. Li, Stephanie Chow, Dallyce Stinton, Tatiana Zaliznyak, Steven R. Beaupre

Faculty Works: Biology, Chemistry, and Environmental Studies

A newmethod tomeasure growth rates of individual photoautotrophic cells by combining stable isotope probing (SIP) and single-cell resonance Raman microspectrometry is introduced. This report explores optimal experimental design and the theoretical underpinnings for quantitative responses of Raman spectra to cellular isotopic composition. Resonance Raman spectra of isogenic cultures of the cyanobacterium, Synechococcus sp., grown in 13C-bicarbonate revealed linear covariance between wavenumber (cm−1) shifts in dominant carotenoid Raman peaks and a broad range of cellular 13C fractional isotopic abundance. Single-cell growth rates were calculated from spectra-derived isotopic content and empirical relationships. Growth rates among any 25 cells in a sample varied …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Sixto E. Portilla

CERCOM Reports

In 2016 the Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visited 9 locations in Great South Bay to monitor for dissolved oxygen (DO), pH, salinity, clarity, depth and temperature. This monitoring program has been conducted for the past 14 years. These parameters are critical in determining long-term water quality conditions in Long Island estuaries. Methodologies for monitoring parameters are provided by the Standard Methods for the Examination of Water and Wastewater 20th Edition (1998). Criteria for determining water quality conditions in marine eco-systems are based on the type of contact (uses) people have with the water system. The …

Great South Bay, Long Island, New York Summer Water Quality Monitoring Program, Cercom, Molloy University, John Tanacredi Ph.D., Sixto E. Portilla

CERCOM Reports

The Center for Environmental Research and Coastal Oceans Monitoring (CERCOM) visits 15 locations in Great South Bay to monitor for dissolved oxygen (DO), pH, salinity, clarity, total and fecal coliforms and temperature. This monitoring program has been conducted for the past 13 years. These parameters are critical in determining long-term water quality conditions in Long Island estuaries. Methodologies for monitoring parameters are provided by the Standard Methods for the Examination of Water and Wastewater 20th Edition (1998). Criteria for determining water quality conditions in marine eco-systems are based on the type of contact (uses) people have with the water system. …