Physical Sciences and Mathematics Commons^™

Sturgeon Chub Distributional Patterns And Habitat Use And Benthic Fish Assemblage Structure In Missouri River Tributaries Of South Dakota, Mitchell R. Magruder

School of Natural Resources: Dissertations, Theses, and Student Research

Native species of the Missouri River drainage inhabiting benthic habitats dominate state and federal lists of species at risk. Sicklefin Chub Macrhybopsis meeki and Sturgeon Chub Macrhybopsis gelida are two native Missouri River benthic minnows that are currently under review for federal listing under the Endangered Species Act (ESA) due to extensive population declines and local extirpations within their native range. Substantial alterations to the Missouri River threaten Macrhybopsis spp. and other benthic fishes; however, large, less impacted tributaries in South Dakota may act as refugia for native species. The extent of Sicklefin Chub and Sturgeon Chub populations is largely …

Human And Hydrologic Influences On Nebraska's Endangered Rainwater Basin Wetlands, Sarah Thompson

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Over half of wetlands in North America have been lost or degraded. Rainwater Basin (RWB) wetlands, located in south-central Nebraska, are a primary example of such loss; an estimated 90% have been destroyed by land conversion for agriculture. Remaining RWB wetlands are often embedded in row-crop fields, where they are threatened by altered surface water runoff flow, drainage features, and excess sediment inputs. Efforts at the state and federal level have been made to preserve this wetland complex due to the critical stopover habitat these wetlands provide for migratory birds. Land managers work to maintain sufficient water levels during migratory …

The North Platte River Valley: The Intersectionality Between Water Quality And People, Anni Poetzl

School of Natural Resources: Dissertations, Theses, and Student Research

The North Platte River (NPR) Valley of western Nebraska is a semi-arid watershed with row crop production, livestock production, and urban land use activity and has a population of diverse stakeholders. These land use activities contribute to the enrichment of surface waters, such as streams, which can affect human and ecosystem health, as well as economic development and recreational activities. The project objectives are to: (1) quantify the movement of dissolved inorganic nutrients from the land within the NPR Valley to the NPR via tributaries and canals, (2) identify spatiotemporal variability of nutrient limitation of periphyton growth within the NPR, …

Earlier Snowmelt May Lead To Late Season Declines In Plant Productivity And Carbon Sequestration In Arctic Tundra Ecosystems, Donatella Zona, Peter M. Lafleur, Koen Hufkens, Barbara Bailey, Beniamino Gioli, George Burba, Jordan P. Goodrich, Anna K. Liljedahl, Eugénie S. Euskirchen, Jennifer D. Watts, Mary Farina, John S. Kimball, Martin Heimann, Mathias Göckede, Martijn Pallandt, Torben R. Christensen, Mikhail Mastepanov, Efrén López-Blanco, Marcin Jackowicz-Korczynski, Albertus J. Dolman, Luca Belelli Marchesini, Roisin Commane, Steven C. Wofsy, Charles E. Miller, David A. Lipson, Josh Hashemi, Kyle A. Arndt, Lars Kutzbach, David Holl, Julia Boike, Christian Wille, Torsten Sachs, Aram Kalhori, Xia Song

Daugherty Water for Food Global Institute: Faculty Publications

Arctic warming is affecting snow cover and soil hydrology, with consequences for carbon sequestration in tundra ecosystems. The scarcity of observations in the Arctic has limited our understanding of the impact of covarying environmental drivers on the carbon balance of tundra ecosystems. In this study, we address some of these uncertainties through a novel record of 119 site-years of summer data from eddy covariance towers representing dominant tundra vegetation types located on continuous permafrost in the Arctic. Here we found that earlier snowmelt was associated with more tundra net CO2 sequestration and higher gross primary productivity (GPP) only in June …

One Health In Action: Flea Control And Interpretative Education At Badlands National Park, David Eads, Lindsey Buehler, Anne Esbenshade, Jason Fly, Evan Miller, Holly Redmond, Emily Ritter, National Park Service, Sasha Wittmann, Paul Roghair, Eddie Childers

United States Geological Survey: Staff Publications

No abstract provided.

A 1.8 Million Year History Of Amazon Vegetation, Andrea K. Kern, Thomas Kenji Akabane, Jaqueline Q. Ferreira, Cristiano M. Chiessi, Debra A. Willard, Fabricio Ferreira, Allan Oliveira Sanders, Cleverson G. Silva, Catherine Rigsby, Francisco W. Cruz, Gary S. Dwyer, Sherilyn C. Fritz, Paul A. Baker

Department of Earth and Atmospheric Sciences: Faculty Publications

During the Pleistocene, long-term trends in global climate were controlled by orbital cycles leading to high amplitude glacial-interglacial variability. The history of Amazonian vegetation during this period is largely unknown since no continuous record from the lowland basin extends significantly beyond the last glacial stage. Here we present a paleoenvironmental record spanning the last 1800 kyr based on palynological data, biome reconstructions, and biodiversity metrics from a marine sediment core that preserves a continuous archive of sediments from the Amazon River.

Tropical rainforests dominated the Amazonian lowlands during the last 1800 ka interchanging with surrounding warm-temperate rainforests and tropical seasonal …

Drought‑Stricken U.S. States Have More Comprehensive Water‑Related Hazard Planning, Theresa Jedd, Kelly Smith

Drought Mitigation Center: Faculty Publications

Despite the devastating impacts of droughts, the United States lacks a national drought plan. This leaves states to address droughts in water, hazard, and stand-alone plans which are designed to reduce drought vulnerability and to prepare government, industry, and society to cope with the impacts. Yet, there is limited empirical research that evaluates the comprehensiveness of these plans, specifically in terms of whether they include preparedness and mitigation measures or triggers for action and response. To fill this gap, this study’s first aim was to establish an evaluation framework based on principles from the drought mitigation literature. The study then …

The Sensitivity Of Meteorological Dynamics To The Variability In Catchment Characteristics, Shimelash Molla Kassaye, Tsegaye Tadesse, Getachew Tegegne, Kindie Engdaw Tadesse

Drought Mitigation Center: Faculty Publications

Evaluating meteorological dynamics is a challenging task due to the variability in hydroclimatic settings. This study is designed to assess the sensitivity of precipitation and temperature dynamics to catchment variability. The effects of catchment size, land use/cover change, and elevation differences on precipitation and temperature variability were considered to achieve the study objective. The variability in meteorological parameters to the catchment characteristics was determined using the coefficient of variation on the climate data tool (CDT). A land use/cover change and terrain analysis was performed on Google Earth Engine (GEE) and ArcGIS. In addition, a correlation analysis was performed to identify …

Do Pharmaceuticals In The Environment Pose A Risk To Wildlife?, Thomas G. Bean, Elizabeth A. Chadwick, Marta Herrero‐Villar, Rafael Mateo, Vinny Naidoo, Barnett A. Rattner

United States Geological Survey: Staff Publications

The vast majority of knowledge related to the question “To what extent do pharmaceuticals in the environment pose a risk to wildlife?” stems from the Asian vulture crisis (>99% decline of some species of Old World vultures on the Indian subcontinent related to the veterinary use of the nonsteroidal anti‐inflammatory drug [NSAID] diclofenac). The hazard of diclofenac and other NSAIDs (carprofen, flunixin, ketoprofen, nimesulide, phenylbutazone) to vultures and other avian species has since been demonstrated; indeed, only meloxicam and tolfenamic acid have been found to be vulture‐safe. Since diclofenac was approved for veterinary use in Spain and Italy in …

A 14,100 Cal B. P. Rocky Mountain Locust Cache From Winnemucca Lake, Pershing County, Nevada, Evan J. Pellegrini, Eugene M. Hattori, Larry Benson, John Southon, Hojun Song, Derek A. Woller

United States Geological Survey: Staff Publications

The remains of approximately 1000 (MNI) Rocky Mountain locusts (Melanoplus spretus) from an archaeological cache pit in Crypt Cave, Winnemucca (dry) Lake, Nevada, date to between 14,305–14,067 calendar years before present (95.4 % confidence; 12,238 ± 18 ¹⁴C yrs. B.P.). The age of this western Great Basin occupation along the shoreline of Lake Lahontan is consistent with occupation of several other Western North American terminal Pleistocene sites dating prior to 14,000 cal. B.P., including distinctive petroglyphs on the western shore of Winnemucca Lake dating as early as 14,800–13,200 cal. B.P.

Population Health Metrics During The Early Stages Of The Covid-19 Pandemic: Correlative Pilot Study, Marie A. Severson, David A. Cassada, Victor C. Huber, Daniel D. Snow, Lisa M. Mcfadden

Nebraska Water Center: Faculty Publications

Background: COVID-19 has caused nearly 1 million deaths in the United States, not to mention job losses, business and school closures, stay-at-home orders, and mask mandates. Many people have suffered increased anxiety and depression since the pandemic began. Not only have mental health symptoms become more prevalent, but alcohol consumption has also increased during this time. Helplines offer important insight into both physical and mental wellness of a population by offering immediate, anonymous, cheap, and accessible resources for health and substance use disorders (SUD) that was unobstructed by many of the mandates of the pandemic. Further, the pandemic also …

Comparative Behavioral Ecotoxicology Of Inland Silverside Larvae Exposed To Pyrethroids Across A Salinity Gradient, Sara J. Hutton, Samreen Siddiqui, Emily I. Pedersen, Christopher Y. Markgraf, Amelie Segarra, Michelle L. Hladik, Richard E. Connon, Susanne M. Brander

United States Geological Survey: Staff Publications

Pyrethroids, a class of commonly used insecticides, are frequently detected in aquatic environments, including estuaries. The influence that salinity has on organism physiology and the partitioning of hydrophobic chemicals, such as pyrethroids, has driven interest in how toxicity changes in saltwater compared to freshwater. Early life exposures in fish to pyrethroids cause toxicity at environmentally relevant concentrations, which can alter behavior. Behavior is a highly sensitive endpoint that influences overall organism fitness and can be used to detect toxicity of environmentally relevant concentrations of aquatic pollutants. Inland Silversides (Menidia beryllina), a commonly used euryhaline model fish species, were exposed …

Using Active Source Seismology To Image The Palos Verdes Fault Damage Zone As A Function Of Distance, Depth, And Geology, Travis Alongi, Emily E. Brodsky, Jared Kluesner, Daniel Brothers

United States Geological Survey: Staff Publications

Fault damage zones provide a window into the non-elastic processes of an earthquake. Geological and seismic tomography methods have been unable to measure damage zones at depth with sufficient spatial sampling to evaluate the relative influence of depth, distance, and lithological variations. Here, we identify and analyze the damage zone of the Palos Verdes Fault offshore southern California using two 3D seismic reflection datasets. We apply a novel algorithm to identify discontinuities attributed to faults and fractures in large seismic volumes and examine the spatial distribution of fault damage in sedimentary rock surrounding the Palos Verdes Fault. Our results show …

Coastal Wetland Area Change For Two Freshwater Diversions In The Mississippi River Delta, John R. White, Brady Couvillion, John W. Day

United States Geological Survey: Staff Publications

Coastal systems around the globe are being re-integrated with adjacent river systems to restore the natural hydrologic connection to riparian wetlands. The Mississippi River sediment diversions or river reconnections are one such tool to combat high rates of wetland loss in coastal Louisiana, USA by providing freshwater, sediment, and nutrients. There has been some disagreement in the published literature whether re-establishing river reconnection is slowing or contributing to coastal wetland loss. This issue is due to the difficulties in the application of remote sensing in low-relief environments where water level changes could indicate either land loss or simply temporary submergence. …

Observations On Late Cretaceous Micrampulla (Corethrales, Bacillariophyceae) From The Campbell Plateau (Zealandia), Southwest Pacific Ocean, Kenta Abe, David M. Harwood, Richard W. Jordan

Department of Earth and Atmospheric Sciences: Faculty Publications

Late Cretaceous (late Campanian) diatom assemblages from the Campbell Plateau (Zealandia), southwest Pacific Ocean, obtained from Deep Sea Drilling Project (DSDP) Leg 29 Site 275, contain well-preserved specimens of two enigmatic diatom species currently assigned to the genus Ktenodiscus; Micrampulla parvula originally described from the Maastrichtian-age Moreno Shale, California, and Pterotheca cretacea from DSDP Site 275. In general, the two species share a number of common features with modern Corethron (domed valves, probable heterovalvate frustules, T-shaped serrated articulated spines, marginal sockets), but differ in the location of the sockets (i.e. vertically at the base of the valve dome and …

Evidence For The ~ 1.4 Ga Picuris Orogeny In The Central Colorado Front Range, Asha A. Mahatma, Yvette D. Kuiper, Christopher S. Holm-Denoma

United States Geological Survey: Staff Publications

We present the first evidence for sedimentation and new evidence for penetrative deformation and metamorphism in the central Colorado Front Range associated with the ~ 1.48–1.35 Ga Picuris orogeny. This orogeny has recently been recognized in New Mexico, Arizona and southern Colorado and may be part of a larger active accretionary margin that includes the ~ 1.51–1.46 Ga Pinware and Baraboo events, in eastern Canada and central US respectively, that preceded the amalgamation of the Rodinian supercontinent. We demonstrate that in addition to ~ 1.4 Ga reactivation of northeast-trending Paleoproterozoic shear zones, regional folding occurred in an area south of …

Level And Pattern Of Overstory Retention Shape The Abundance And Long-Term Dynamics Of Natural And Created Snags, Charles B. Halpern, Allison K. Rossman, Joan C. Hagar

United States Geological Survey: Staff Publications

Standing dead trees, or snags, serve myriad functions in natural forests, but are often scarce in forests managed for timber production. Variable retention (VR), the retention of live and dead trees through harvest, has been adopted globally as a less intensive form of regeneration harvest. In this study, we explore how two key elements of VR systems — level (amount) and spatial pattern of live-tree retention — affect the carryover and post-harvest dynamics of natural and artificially created snags. We present nearly two decades of data from the DEMO Study, a regional-scale experiment in VR harvests of Douglas-fir-dominated forests in …

Hydrogen Isotope Behavior During Rhyolite Glass Hydration Under Hydrothermal Conditions, Michael R. Hudak, Ilya N. Bindeman, James M. Watkins, Jacob B. Lowenstern

United States Geological Survey: Staff Publications

The diffusion of molecular water (H₂O_m) from the environment into volcanic glass can hydrate the glass up to several wt% at low temperature over long timescales. During this process, the water imprints its hydrogen isotope composition (δD_H2O) to the glass (δD_gl) offset by a glass-H₂O fractionation factor (ΔD_gl-H2O = δD_gl – δD_H2O) which is approximately -33‰ at Earth surface temperatures. Glasses hydrate much more rapidly at higher, sub-magmatic temperatures as they interact with H₂O during eruption, transport, and emplacement. To aid in the interpretation …

Getting Ahead Of Flash Drought: From Early Warning To Early Action, Jason A. Otkin, Molly Woloszyn, Hailan Wang, Mark D. Svoboda, Marina Skumanich, Roger Pulwarty, Joel Lisonbee, Andrew Hoell, Mike Hobbins, Tonya Haigh, Amanda E. Cravens

Drought Mitigation Center: Faculty Publications

Flash droughts, characterized by their unusually rapid intensification, have garnered increasing attention within the weather, climate, agriculture, and ecological communities in recent years due to their large environmental and socioeconomic impacts. Because flash droughts intensify quickly, they require different early warning capabilities and management approaches than are typically used for slower-developing “conventional” droughts. In this essay, we describe an integrated research-and-applications agenda that emphasizes the need to reconceptualize our understanding of flash drought within existing drought early warning systems by focusing on opportunities to improve monitoring and prediction. We illustrate the need for engagement among physical scientists, social scientists, operational …

Soils Of The Central Nebraska Loess Hills And Central Loess Plains, Judith Turk, Rebecca Young, Nicolas A. Jelinsky, Amber D. Anderson, Ashlee Dere, Colby J. Moorberg, Rachel K. Owen

Conservation and Survey Division

Understanding soil systems that characterize a region is critical to natural resource management. However, the knowledge gained through intensive study of local soil systems, which takes place annually as part of collegiate soil judging contests, is often poorly preserved for future use. In this study, field descriptions and laboratory data for 16 soil profiles described for the 2019 Region 5 Soil Judging Contest were used to characterize the soil system of the Central Nebraska Loess Hills and Central Loess Plains. Three landscape components of this soil system were analyzed: the loess uplands and rainwater basins, the transitional zone, and bottomlands. …

Drivers Of Pb, Sb And As Release From Spent Gunshot In Wetlands: Enhancement By Organic Matter And Native Microorganisms, Anna Potysz, Łukasz J. Binkowski, Jakub Kierczak, Barnett A. Rattner

United States Geological Survey: Staff Publications

In many countries the use of lead-based ammunition is prevalent, and results in exposure and poisoning of waterfowl and other species of birds. In waterfowl hunting areas large quantities of spent shot may be deposited in wetland and terrestrial habitats. These pellets can undergo transformations, which are influenced by various abiotic and biotic factors. In addition to lead (Pb), other elements like antimony (Sb) and arsenic (As) can be leached from Pb shot into the environment. In vitro simulations that included organic matter and microorganisms were utilized to examine elemental leaching from gunshot. We found that leaching efficiency was the …

Limits To Coseismic Landslides Triggered By Cascadia Subduction Zone Earthquakes, Alex R.R. Grant, William T. Struble, Sean R. Lahusen

United States Geological Survey: Staff Publications

Landslides are a significant hazard and dominant feature throughout the landscape of the Pacific Northwest. However, the hazard and risk posed by coseismic landslides triggered by great Cascadia Subduction Zone (CSZ) earthquakes is highly uncertain due to a lack of local and global data. Despite a wealth of other geologic evidence for past earthquakes on the Cascadia Subduction Zone, no landslides have been definitively linked to such earthquakes, even in areas otherwise highly susceptible to failure. While shallow landslides may not leave a lasting topographical signature in the landscape, there are thousands of deep-seated landslides in Cascadia, and these deposits …

Neural Net Detection Of Seismic Features Related To Gas Hydrates And Free Gas Accumulations On The Northern U.S. Atlantic Margin, Urmi Majumdar, Nathaniel C. Miller, Carolyn D. Ruppel

United States Geological Survey: Staff Publications

Bottom-simulating reflections (BSRs) that sometimes mark the base of the gas hydrate stability zone in marine sediments are often identified based on the reverse polarity reflections that cut across stratigraphic layering in seismic amplitude data. On the northern U.S. Atlantic margin (USAM) between Cape Hatteras and Hudson Canyon, legacy seismic data have revealed pronounced BSRs south of the deepwater extension of Hudson Canyon and more subtle ones from offshore Delaware south to Cape Hatteras, where the reflections sometimes follow stratigraphic layering. Using high-resolution seismic data acquired during the 2018 Mid-Atlantic Resource Imaging Experiment and a supervised neural net, we identify …

Establishment Of Invasive Black Carp (Mylopharyngodon Piceus) In The Mississippi River Basin: Identifying Sources And Year Classes Contributing To Recruitment, Gregory W. Whitledge, Patrick T. Kroboth, Duane C. Chapman, Quinton E. Phelps, Wes Sleeper, Jennifer Bailey, Jill A. Jenkins

United States Geological Survey: Staff Publications

Black Carp (Mylopharyngodon piceus) was imported to the USA to control aquaculture pond snails. This species has escaped captivity and occurs in parts of the Mississippi River, several tributaries, and floodplain lakes, which is concerning due to potential competition with native fishes and predation on native mussels, many of which are imperiled. However, Black Carp captures have primarily been incidental by commercial fishers, and evidence of reproduction in the wild is limited. The objectives of this study were to assess relative abundance of aquaculture- origin and wild Black Carp using ploidy and otolith stable isotope analysis, identify spatial …

Very High Middle Miocene Surface Productivity On The U.S. Mid-Atlantic Shelf Amid Glacioeustatic Sea Level Variability, Marci M. Robinson, Harry J. Dowsett, Timothy D. Herbert

United States Geological Survey: Staff Publications

The Miocene Climatic Optimum (MCO) provides important insights into how the climate system operates under elevated temperatures and atmospheric CO₂ levels. Few western North Atlantic paleotemperature or paleoecological records exist from the MCO, despite their importance for understanding both regional and global climate dynamics. Here we present quantitative MCO paleoecological data from the western North Atlantic, specifically from the Baltimore Gas & Electric (BG&E) marine sediment core from southern Maryland, USA. We examine alkenones and planktic foraminifera and document the first sea surface temperature (SST) and productivity estimates for the MCO and the Middle Miocene Climate Transition (MMCT) from …

Discovering Hidden Geothermal Signatures Using Non-Negative Matrix Factorization With Customized K-Means Clustering, V. V. Vesselinov, B. Ahmmed, M. K. Mudunuru, J. D. Pepin, E. R. Burns, D. L. Siler, S. Karra, R. S. Middleton

United States Geological Survey: Staff Publications

Discovery of hidden geothermal resources is challenging. It requires the mining of large datasets with diverse data attributes representing subsurface hydrogeological and geothermal conditions. The commonly used play fairway analysis approach typically incorporates subject-matter expertise to analyze regional data to estimate geothermal characteristics and favorability. We demonstrate an alternative approach based on machine learning (ML) to process a geothermal dataset from southwest New Mexico (SWNM). The study region includes low- and medium-temperature hydrothermal systems. Several of these systems are not well characterized because of insufficient existing data and limited past explorative work. This study discovers hidden patterns and relations in …

Multi-Omics Responses In Tree Swallow (Tachycineta Bicolor) Nestlings From The Maumee Area Of Concern, Maumee River, Ohio, Chi Yen Tseng, Christine M. Custer, Thomas W. Custer, Paul M. Dummer, Natalie Karouna-Renier, Cole W. Matson

United States Geological Survey: Staff Publications

A multi-omics approach was utilized to identify altered biological responses and functions, and to prioritize contaminants to assess the risks of chemical mixtures in the Maumee Area of Concern (AOC), Maumee River, OH, USA. The Maumee AOC is designated by the United States Environmental Protection Agency as having significant beneficial use impairments, including degradation of fish and wildlife populations, bird or animal deformities or reproduction problems, and loss of fish and wildlife habitat. Tree swallow (Tachycineta bicolor) nestlings were collected at five sites along the Maumee River, which included wastewater treatment plants (WWTPs) and industrial land-use sites. Polychlorinated …

Coastalimagelib: An Open- Source Python Package For Creating Common Coastal Image Products, Maile P. Mccann, Dylan L. Anderson, Christopher R. Sherwood, Brittany Bruder, A. Spicer Bak, Katherine L. Brodie

United States Geological Survey: Staff Publications

CoastalImageLib is a Python library that produces common coastal image products intended for quantitative analysis of coastal environments. This library contains functions to georectify and merge multiple oblique camera views, produce statistical image products for a given set of images, and create subsampled pixel instruments for use in bathymetric inversion, surface current estimation, run-up calculations, and other quantitative analyses. This package intends to be an open-source broadly generalizable front end to future coastal imaging applications, ultimately expanding user accessibility to optical remote sensing of coastal environments. This package was developed and tested on data collected from the Argus Tower, a …

A Systematic Review And Meta-Analysis Of The Direct Effects Of Nutrients On Corals, Eileen M. Nalley, Lillian J. Tuttle, Emily E. Conklin, Alexandria L. Barkman, Devynn M. Wulstein, Madeline C. Schmidbauer, Megan J. Donahue

United States Geological Survey: Staff Publications

Chronic exposure of coral reefs to elevated nutrient conditions can modify the performance of the coral holobiont and shift the competitive interactions of reef organisms. Many studies have nowquantified the links between nutrients and coral performance, but fewhave translated these studies to directly address coastal water quality standards. To address this management need, we conducted a systematic review of peer-reviewed studies, public reports, and gray literature that examined the impacts of dissolved inorganic nitrogen (DIN: nitrate, nitrite, and ammonium) and dissolved inorganic phosphorus (DIP: phosphate) on scleractinian corals. The systematic review resulted in 47 studies with comparable data on coral …

Transcriptome Signatures Of Wastewater Effluent Exposure In Larval Zebrafish Vary With Seasonal Mixture Composition In An Effluent-Dominated Stream, Emma B. Meade, Luke R. Iwanowicz, Nicklaus Neureuther, Gregory H. Lefevre, Dana W. Kolpin, Hui Zhi, Shannon M. Meppelink, Rachael F. Lane, Angela Schmoldt, Aurash Mohaimani, Olaf Mueller, Rebecca D. Klaper

United States Geological Survey: Staff Publications

Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic and terrestrial organisms but also continually expose them to complex mixtures of pharmaceuticals that can potentially impair growth, behavior, and reproduction. Currently, few biomarkers are available that relate to pharmaceutical-specific mechanisms of action. In the experiment reported in this paper, zebrafish (Danio rerio) embryos at two developmental stages were exposed to water samples from three sampling sites (0.1 km upstream of the outfall, at the effluent outfall, and 0.1 km below the outfall) during base-flow conditions from two months (January and May) of a temperate-region effluent-dominated stream …