Physical Sciences and Mathematics Commons^™

Diffsplice: The Genome-Wide Detection Of Differential Splicing Events With Rna-Seq, Yin Hu, Yan Huang, Ying Du, Christian F. Orellana, Darshan Singh, Amy R. Johnson, Anaïs Monroy, Pei-Fen Kuan, Scott M. Hammond, Liza M. Hammond, Scott H. Randell, Derek Y. Chiang, D. Neil Hayes, Corbin Jones, Yufeng Liu, Jan F. Prins, Jinze Liu

Computer Science Faculty Publications

The RNA transcriptome varies in response to cellular differentiation as well as environmental factors, and can be characterized by the diversity and abundance of transcript isoforms. Differential transcription analysis, the detection of differences between the transcriptomes of different cells, may improve understanding of cell differentiation and development and enable the identification of biomarkers that classify disease types. The availability of high-throughput short-read RNA sequencing technologies provides in-depth sampling of the transcriptome, making it possible to accurately detect the differences between transcriptomes. In this article, we present a new method for the detection and visualization of differential transcription. Our approach does …