Physical Sciences and Mathematics Commons^™

Transcriptional Responses To Estrogen And Progesterone In Mammary Gland Identify Networks Regulating P53 Activity, Shaolei Lu, Klaus A. Becker, Mary J. Hagen, Haoheng Yan, Amy L. Roberts, Lesley A. Mathews, Sallie S. Schneider, Hava Siegelmann, Kyle J. Macbeth, Stephen M. Tirrell, Jeffrey L. Blanchard, D. Joseph Jerry

Hava Siegelmann

Estrogen and progestins are essential for mammary growth and differentiation but also enhance the activity of the p53 tumor suppressor protein in the mammary epithelium. However, the pathways by which these hormones regulate p53 activity are unknown. Microarrays were used to profile the transcriptional changes within the mammary gland after administration of either vehicle, 17β-estradiol (E), or progesterone (P) individually and combined (EP). Treatment with EP yielded 1182 unique genes that were differentially expressed compared to the vehicle-treated group. Although 30% of genes were responsive to either E or P individually, combined treatment with both EP had a synergistic effect …