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Ozonized Biochar Filtrate Effects On The Growth Of Pseudomonas Putida And Cyanobacteria Synechococcus Elongatus Pcc 7942, Oumar Sacko, Nancy L. Engle, Timothy J. Tschaplinski, Sandeep Kumar, James Weifu Lee

Chemistry & Biochemistry Faculty Publications

Background

Biochar ozonization was previously shown to dramatically increase its cation exchange capacity, thus improving its nutrient retention capacity. The potential soil application of ozonized biochar warrants the need for a toxicity study that investigates its effects on microorganisms.

Results

In the study presented here, we found that the filtrates collected from ozonized pine 400 biochar and ozonized rogue biochar did not have any inhibitory effects on the soil environmental bacteria Pseudomonas putida, even at high dissolved organic carbon (DOC) concentrations of 300 ppm. However, the growth of Synechococcus elongatus PCC 7942 was inhibited by the ozonized biochar filtrates at …