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Full-Text Articles in Physical Sciences and Mathematics
Bovine Serum Albumin Detection By Using Molecularly Imprinted Surface Plasmon Resonance Sensors, Ali̇ Araz
Bovine Serum Albumin Detection By Using Molecularly Imprinted Surface Plasmon Resonance Sensors, Ali̇ Araz
Turkish Journal of Chemistry
Molecular imprinted polymers (MIP) have key-lock pattern binding properties specific to the size and shape of target molecules. In this study, we have prepared detection platforms based on a molecularly imprinted surface plasmon resonance (SPR) sensor that can detect bovine serum albumin (BSA) sensitively, selectively, quickly, and in real time. The polymeric film prepared on the SPR sensor surface by molecular imprinting method was obtained by selecting the N-methacryloyl-(L)-glutamic acid molecule as a suitable functional monomer using ultraviolet polymerization. Three different imprinting methods, such as epitope, bulk, and surface imprinting methods, were used to examine the imprinting efficiency. Real-time measurements …
Adsorption Of Bovine Serum Albumin (Bsa) By Bare Magnetite Nanoparticles With Surface Oxidative Impurities That Prevent Aggregation, Somayeh Rahdar, Abbas Rahdar, Shahin Ahmadi, John F. Trant
Adsorption Of Bovine Serum Albumin (Bsa) By Bare Magnetite Nanoparticles With Surface Oxidative Impurities That Prevent Aggregation, Somayeh Rahdar, Abbas Rahdar, Shahin Ahmadi, John F. Trant
Chemistry and Biochemistry Publications
Bare, uncoated magnetite nanoparticles, synthesized using an electrochemical surfactant-free synthesis, have highly oxidized surfaces that prevent aggregation. These particles have demonstrated highly intriguing biological activity showing extremely potent antibiotic activity against both gram-positive and gram-negative bacteria with little toxicity to rats. This difference in activity could be ascribed to the nature of the protein corona. In this study the kinetics and thermodynamics of the binding of bovine serum albumin, used as a model serum protein, to these magnetite nanoparticles was analyzed. There is no significant change in particle diameter by dynamic light scattering following adsorption indicating corona formation does not …
Investigation Of The Photophysical And Photochemical Behavior Of Substituted Zinc Phthalocyanines And Their Water-Soluble Quaternized Derivatives, Gülsev Di̇lber, Mahmut Durmuş, Hali̇t Kanteki̇n
Investigation Of The Photophysical And Photochemical Behavior Of Substituted Zinc Phthalocyanines And Their Water-Soluble Quaternized Derivatives, Gülsev Di̇lber, Mahmut Durmuş, Hali̇t Kanteki̇n
Turkish Journal of Chemistry
New nonperipherally and peripherally tetrasubstituted zinc(II) phthalocyanine complexes containing 1-benzylpiperidin-4-ol groups and their quaternized derivatives are reported in this work. These complexes were characterized successfully and their photophysical and photochemical properties were studied. These properties were determined in dimethylsulfoxide for all studied complexes and in water and water $+$ Triton X-100 solutions for ionic Zn(II) phthalocyanines. All studied zinc(II) phthalocyanine complexes generated high singlet oxygen, especially in dimethylsulfoxide, and these properties make them potential photosensitizers for use in the photodynamic therapy of cancer. The effects of the position and quaternization of the substituents on the photophysical and photochemical properties were …
Ammonium Sulfate Precipitation Combined With Liquid Chromatography Is Sufficient For Purification Of Bovine Serum Albumin That Is Suitable For Most Routine Laboratory Applications, Odutayo O. Odunuge, Alina Shazhko
Ammonium Sulfate Precipitation Combined With Liquid Chromatography Is Sufficient For Purification Of Bovine Serum Albumin That Is Suitable For Most Routine Laboratory Applications, Odutayo O. Odunuge, Alina Shazhko
Faculty Publications
The use of bovine serum albumin (BSA) in routine biochemical assays such as restriction enzyme digestion and immunodetection is plagued largely by two common contaminants, DNase and immunoglobulins G (IgGs). Acetylation of BSA to inactivate DNase limits its use as a protein standard due to interference with color development in assays such as Lowry’s. In spite of the availability of inexpensive BSA, its purification involves several cumbersome and time-consuming steps. In this work, we employed a modified strategy of ammonium sulfate precipitation coupled with liquid chromatography to purify BSA that is free of DNase and IgGs. Purified BSA tested negative …
Blocking Method In Enzyme-Amplified Amperometric Dna Biosensor, Miao He, Wei Chen, Xiong-Wei Xu, Ai-Lin Liu, Xin-Hua Lin
Blocking Method In Enzyme-Amplified Amperometric Dna Biosensor, Miao He, Wei Chen, Xiong-Wei Xu, Ai-Lin Liu, Xin-Hua Lin
Journal of Electrochemistry
Non-specific absorption of enzyme and nucleotide is a main source leading to the background staining in enzyme-amplified amperometric detection of DNA. To eliminate the background staining in this system, the blocking procedure by mercapto-hexanol ( MCH) and bovine serum albumin ( BSA) was studied systematically. The results show that the anti-fouling effect of BSA is better than that of MCH. A simple and efficient blocking strategy employing BSA as the sole blocking reagent was established and applied in the detection of PMLRARα fusion gene in acute promyelocytic leukemia. It can effectively eliminate the background staining,shorten the pre-processing time,and achieve strong …