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Full-Text Articles in Physical Sciences and Mathematics
Exploring The Mechanism Of The Electrostatic Denaturation Of Double-Stranded Dna, Gayatri Raghu
Exploring The Mechanism Of The Electrostatic Denaturation Of Double-Stranded Dna, Gayatri Raghu
Master's Theses
Electrostatic melting is an electrochemical tool that can be used to analyze the stability of DNA, allowing for the detection of various mutations in double-stranded DNA (dsDNA). Here, we explore the influence of electrostatic double layer formation on the unzipping of dsDNA to better comprehend the mechanism of this process. Previous studies by our lab show that the melting curve produced can distinguish between fully complementary 34-bp strands and a version of the same sequence in which one base pair has been replaced with a mismatch pair or detect and characterize the crosslinking of the dsDNA by anticancer drug cisplatin. …
Effects Of Crowding Agents On I-Motif Dna, Hayden Brines
Effects Of Crowding Agents On I-Motif Dna, Hayden Brines
Honors Theses
Deoxyribonucleic acid (DNA) is a well-known double stranded, helical, biological molecule. In addition to its more commonly known structure, DNA can also form more complicated structures like G-quadruplexes and i-motifs (iM). The iMs are formed by cytosine rich DNA and are a four stranded structure that is typically looped around itself. The iM formation is typically pH-dependent and is favored in more acidic conditions; the pKa value is approximately 6.5. This pKa value allows for potential in vivo formation, since the cells have a pH of approximately 7.3. Due to this, iMs are thought to be powerful, innovative molecules for …
Utilizing Raman Spectroscopy To Determine The Time Since Deposition Of Heated Bloodstains, Alexis Pearl Barber
Utilizing Raman Spectroscopy To Determine The Time Since Deposition Of Heated Bloodstains, Alexis Pearl Barber
Legacy Theses & Dissertations (2009 - 2024)
The development of novel forensic methods is a rapidly growing area within analytical chemistry. Analytical chemistry is well suited for forensic analyses as a plethora of information can be ascertained from utilizing a wide variety of techniques. At the scene of violent crimes, bloodstain evidence is one of the most frequently found and valuable types of evidence. This is because blood is a complex biological fluid which can provide crucial information during a forensic investigation including DNA evidence. The most informative form of analysis is DNA profiling, which potentially allows for the identification of an individual. However, there is more …