Open Access. Powered by Scholars. Published by Universities.®
Physical Sciences and Mathematics Commons™
Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 4 of 4
Full-Text Articles in Physical Sciences and Mathematics
Design, Synthesis And Characterization Of Chain-End Functionalized Glyco-Polymer For Efficient Proteomic Analysis, Satya Nandana Narla
Design, Synthesis And Characterization Of Chain-End Functionalized Glyco-Polymer For Efficient Proteomic Analysis, Satya Nandana Narla
ETD Archive
No abstract provided.
Investigating The Interaction Of Zinc-Finger Peptides And Nicked Dna Using Phage Display, Rachel Maria Guerra
Investigating The Interaction Of Zinc-Finger Peptides And Nicked Dna Using Phage Display, Rachel Maria Guerra
Honors Theses
Recognition of structural features of DNA such as gaps, nicks and abasic sites is critical for many DNA-binding proteins, such as those involved in DNA damage repair. This study explores a novel strategy to model these protein-DNA interactions using phage display. Phage display has been commonly used to identity zinc fingers that bind to DNA sequences, but selection of peptides that binding to specific DNA structures has not been reported in the literature. A phage library of 109 variants was created based on one of three zinc finger domains of the DNA repair protein poly (ADP-ribose) polymerase I. The library, …
Biomaterial-Induced Osteogenesis Of Mesenchymal Stem Cells By Surface Roughness And Functionalization, Pongkwan Sitasuwan
Biomaterial-Induced Osteogenesis Of Mesenchymal Stem Cells By Surface Roughness And Functionalization, Pongkwan Sitasuwan
Theses and Dissertations
For tissue engineering and regenerative medicine, it is important to understand factors directing the stem cell fate, including self-renewal, proliferation, differentiation, and apoptosis. Bone marrow derived mesenchymal stem cells (BMSCs) have the potential to differentiate into osteoblasts, chondrocytes, adipocytes, and smooth muscle cells. Specifically, in the field of bone tissue engineering, BMSCs are commonly used for in vitro osteogenesis studies. However the mechanisms and signaling pathways that these cells recognize material surface and utilize to differentiate are still unclear. This dissertation focuses on investigating the effect of surface nanotopography and functionalization on the promotion of osteogenic differentiation of BMSCs. Surface …
Plant Virus Based Materials For Cell Alignment And Differentiation, Elizabeth Balizan
Plant Virus Based Materials For Cell Alignment And Differentiation, Elizabeth Balizan
Theses and Dissertations
This research focused on developing and using plant virus based scaffolds to understand substrate control over cell alignment and differentiation. The first part of this work centered on development of virus based patterns that were then used to align and elongate aortic smooth muscle cells (SMCs). Virus patterns were generated in capillary tubes via a simple drying method. Three experimental parameters were used to control pattern formation: (1) protein concentration, (2) salt concentration, and (3) hydrophobicity of the pre-deposition surface. By controlling these parameters several aspects of the final virus patterns were controlled. First, virus orientation was controlled. Patterns were …