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Full-Text Articles in Physical Sciences and Mathematics
Flanking Sequences Modulate Diepoxide Cross-Linking Efficiencies At The 5'-Gnc Sste, Gregory A. Sawyer
Flanking Sequences Modulate Diepoxide Cross-Linking Efficiencies At The 5'-Gnc Sste, Gregory A. Sawyer
Honors Theses
Diepoxybutane, diepoxyoctane, and mechlorethamine are cytotoxic DNA crosslinking agents that vary in their carcinogenic versus chemotherapeutic potentials. Interstrand cross-linking occurs between the N7 positions of deoxyguanosine residues on opposite strands of the DNA duplex. Each synthetic DNA oligomer used in this study contained four 5'-N\GN2CN3 sites (within a 32 base sequence) and we have systematically varied the bases in the N1 and N2 positions to determine the resulting cross-linking efficiencies of each cytotoxic agent. Each duplex was 5' -end labeled and incubated with cross-linking agent. Interstrand cross-links were purified through denaturing polyacrylamide gel electrophoresis and then subjected to piperidine cleavage. …
Double Stranded Dna-Binding Studies Of Potential Rhodium(Ll) Antitumor Complexes, Amity Elizabeth Burr
Double Stranded Dna-Binding Studies Of Potential Rhodium(Ll) Antitumor Complexes, Amity Elizabeth Burr
Honors Theses
In 1952, Dwyer and coworkers began testing a series of metal complexes for potential inhibition of cancer cell proliferation in animals.[l] The complexes tested were unsuitable for such studies due to their high toxicity. Therefore, no further work was done on the project. However, in 1965, Rosenberg and coworkers revisited the possibility of potential metal-based drugs. Serendipitously, they discovered that cis-diamminedichloroplatinum(lI) (cisplatin) inhibits cell division in E. coli.[2] Further studies of this and other platinum compounds revealed inhibition of tumor cell lines sarcoma 180 and leukemia LI2l0 in mice.[l] Cisplatin was approved by the Food and Drug Administration in 1970 …