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Full-Text Articles in Physical Sciences and Mathematics
Luteolin Inhibits Proliferation, Triggers Apoptosis And Modulates Akt/Mtor And Map Kinase Pathways In Hela Cells, Ritu Raina, Sreepoorna Pramodh, Naushad Rais, Shafiul Haque, Jasmin Shafarin, Khuloud Bajbouj, Mawieh Hamad, Arif Hussain
Luteolin Inhibits Proliferation, Triggers Apoptosis And Modulates Akt/Mtor And Map Kinase Pathways In Hela Cells, Ritu Raina, Sreepoorna Pramodh, Naushad Rais, Shafiul Haque, Jasmin Shafarin, Khuloud Bajbouj, Mawieh Hamad, Arif Hussain
All Works
Flavonoids, a subclass of polyphenols, have been shown to be effective against several types of cancer, by decreasing proliferation and inducing apoptosis. Therefore, the aim of the present study was to assess the anti-carcinogenic potential of luteolin on HeLa human cervical cancer cells, through the use of a cell viability assay, DNA fragmentation assay, mitochondrial membrane potential assay, cell cycle analysis using Annexin/PI staining and flow cytometry, gene expression analysis and a protein profiling array. Luteolin treatment exhibited cytotoxicity towards HeLa cells in a dose- and time-dependent manner, and its anti-proliferative properties were confirmed by accumulation of luteolin-treated cells in …
Tnf-R1 And Fadd Mediate Uvb-Induced Activation Of K+ Channels In Corneal Epithelial Cells, Peter M. Boersma, Loren D. Haarsma, Mark P. Schotanus, John L. Ubels
Tnf-R1 And Fadd Mediate Uvb-Induced Activation Of K+ Channels In Corneal Epithelial Cells, Peter M. Boersma, Loren D. Haarsma, Mark P. Schotanus, John L. Ubels
University Faculty Publications and Creative Works
The goal of this study was to elucidate the role of Fas, TNF-R1, FADD and cytochrome c in UVB-induced K+ channel activation, an early step in UVB-induced apoptosis, in human corneal limbal epithelial (HCLE) cells. HCLE cells were treated with Fas, TNF-R1 or FADD siRNA and exposed to 80 or 150 mJ/cm2 UVB. K+ channel activation and loss of intracellular K+ were measured using whole-cell patch-clamp recording and ion chromatography, respectively. Cytochrome c was measured with an ELISA kit. Cells in which Fas was knocked down exhibited identical UVB-induced K+ channel activation and loss of intracellular K+ to control cells. …
The Redox Couple Of The Cytochrome C Cyanide Complex: The Contribution Of Heme Iron Ligation To The Structural Stability, Chemical Reactivity, And Physiological Behavior Of Horse Cytochrome C, Abel Schejter, Michael D. Ryan, Erica R. Blizzard, Chongyao Zhang, Emanuel Margoliash, Benjamin A. Feinberg
The Redox Couple Of The Cytochrome C Cyanide Complex: The Contribution Of Heme Iron Ligation To The Structural Stability, Chemical Reactivity, And Physiological Behavior Of Horse Cytochrome C, Abel Schejter, Michael D. Ryan, Erica R. Blizzard, Chongyao Zhang, Emanuel Margoliash, Benjamin A. Feinberg
Chemistry Faculty Research and Publications
Contrary to most heme proteins, ferrous cytochrome c does not bind ligands such as cyanide and CO. In order to quantify this observation, the redox potential of the ferric/ferrous cytochrome c–cyanide redox couple was determined for the first time by cyclic voltammetry. Its E0′ was −240 mV versus SHE, equivalent to −23.2 kJ/mol. The entropy of reaction for the reduction of the cyanide complex was also determined. From a thermodynamic cycle that included this new value for the cyt c cyanide complex E0′, the binding constant of cyanide to the reduced protein was estimated to …