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Full-Text Articles in Physical Sciences and Mathematics
Engineering Class A Sortases: Activity And Selectivity Of Hybrid And Ancestral Variants, Sarah Struyvenberg
Engineering Class A Sortases: Activity And Selectivity Of Hybrid And Ancestral Variants, Sarah Struyvenberg
WWU Graduate School Collection
Bacterial sortases are cysteine transpeptidases that anchor virulence factors to the surface of bacterial cells. Sortases are a powerful tool utilized for protein engineering that allow researchers to modify proteins at the protein level, not the DNA level. However, important limitations to utilization of sortases for engineering purposes exist; namely, SrtA from S. aureus is a relatively modest enzyme compared to other SrtA enzymes and is very specific for the LPXTG motif. Previous work from our collaborators and others revealed that sortases from different species can recognize alternative sequences and that activities can vary widely. We were curious about how …
Isopeptide Ligations Catalyzed By Streptococcus Suis Sortase A, Sarah Bowersox
Isopeptide Ligations Catalyzed By Streptococcus Suis Sortase A, Sarah Bowersox
WWU Graduate School Collection
Chemically modified proteins are critical components of modern therapeutics and basic research. To generate non-natural protein derivatives, bacterial sortase enzymes have been effective due to their ability to catalyze selective ligations between protein targets and functional groups that are uncommon in nature. Thus far, the enzymatic approach using sortase has been limited to modifications at the termini of peptide chains. Here we describe efforts to develop a sortase-mediated strategy for the formation of isopeptide bonds at the side chains of internal lysine residues. To this end, we have identified a sortase A homolog from Streptococcus suis (SrtAsuis) that …