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Applications Of Gas Chromatography/Mass Spectrometry And Capillary Electrophoresis For The Analysis Of Lignocellulosic Biomass Pretreatment, Dawn M. Kato
Theses and Dissertations--Chemistry
The focus of this dissertation centers on the development and applications of gas chromatography/mass spectrometry and capillary electrophoresis methodologies to quantify monomeric compositions of the β-O-4 linkages in lignin. Pretreatment is a required step in the utilization of lignocellulosic biomass for biofuels. Lignin is the target of pretreatment because it hinders the accessibility of enzymes and chemicals to cellulose. The effects of pretreatment are commonly assessed utilizing enzymatic saccharification and lignin assays. However, these techniques do not elucidate the effects of pretreatment on the monomeric make up of lignin.
The overarching hypothesis of this dissertation is that changes in individual …