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Full-Text Articles in Physical Sciences and Mathematics
Crispr/Cas9 In Leishmania Genome Editing : Towards A Proof Of Concept, Zein Kasbo
Crispr/Cas9 In Leishmania Genome Editing : Towards A Proof Of Concept, Zein Kasbo
Theses, Dissertations and Culminating Projects
Leishmaniasis is a parasitic Neglected Tropical Disease (NTD) that affects mostly poor countries of the third word due to their limited access to clean water and health care. The disease is transmitted through the bite of a sand fly of the genus Phlebotomus in the Old World, and of the genus Lutzomyia in the New World. Historically, NTDs have not been given adequate attention or research funding and finding curative treatments has been slow. However, the fact that the insect vector and the parasite can live and thrive in any ecological system in the world, and under the current circumstances …
A Computational Study Of C-Cn Bond Activation Through Nickel Catalysis Using The Random Phase Approximation, Craig Waitt
A Computational Study Of C-Cn Bond Activation Through Nickel Catalysis Using The Random Phase Approximation, Craig Waitt
Theses, Dissertations and Culminating Projects
This study evaluates the performance of a computational method known as the Random Phase Approximation (RPA) for a Nickel catalyst that activates the C-CN bond of benzonitrile. The reaction mechanism contains two distinct processes. The first process describes the cleavage of the C-CN bond of benzonitrile. The latter describes the fluxionality of benzonitrile about the Nickel atom. Many popular computational methods would struggle to properly describe either or both processes, because they may not perform consistently for transition-metal complexes, are computationally expensive, or have a strong dependence on orbital energy gaps. The RPA provides results with an excellent balance of …
The Mechanism Of Inhibition Of Botulinum Neurotoxin Type A By Two Quinolinol Compounds, Yacoba Vroom Teschemaker Minnow
The Mechanism Of Inhibition Of Botulinum Neurotoxin Type A By Two Quinolinol Compounds, Yacoba Vroom Teschemaker Minnow
Theses, Dissertations and Culminating Projects
Quinolinol-based compounds are a promising starting point for discovery o f effective inhibitors o f the clostridial neurotoxin, botulinum neurotoxin type A light chain (BoNT/A LC). Insights into the mechanism o f inhibition by quinolinol compounds facilitate interpretation o f docking data and inhibitor optimization. In this study, a fluorogenic substrate o f BoNT/A, SNAPtide, was used to study the mechanism by which two new quinolinol compounds, MSU58 and MSU84, with IC50 values of 3.3 uM and 5.8 uM, respectively, inhibit BoNT/A LC. Kinetic studies and model discrimination analysis showed both compounds to be competitive inhibitors o f BoNT/A …
Investigation Of Hsp90 Structure-Activity Relationships In Epigallocatechin Gallate, Arpita Patel
Investigation Of Hsp90 Structure-Activity Relationships In Epigallocatechin Gallate, Arpita Patel
Theses, Dissertations and Culminating Projects
(-)-Epigallocatechin gallate (EGCG) is a polyphenolic natural product that inhibits the key chaperone protein Hsp90 by binding to the carboxy terminal portion of the protein. The natural product has modest activity as an Hsp90 inhibitor and there is little information in the literature on structure activity relationships for EGCG analogs. Previous studies from this laboratory provided initial data on the effect of a limited range of changes associated with functional groups, substituents and stereochemistry. The focus of this research is to explore these initial results in more detail with a focus on the synthesis of EGCG analogs with more drug …